Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation
•Gelatin was isolated from sea food processing by-product, skate skin.•Two active peptides MVGSAPGVL (829Da) and LGPLGHQ (720Da) were isolated.•Peptides showed potent ACE inhibitory activity (IC50 3.09 and 4.22μM).•Peptides protected human endothelial cells from oxidative damage.•Peptides could be u...
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| Veröffentlicht in: | Food chemistry 2014-01, Vol.143, p.246-255 |
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| creator | Ngo, Dai-Hung Ryu, BoMi Kim, Se-Kwon |
| description | •Gelatin was isolated from sea food processing by-product, skate skin.•Two active peptides MVGSAPGVL (829Da) and LGPLGHQ (720Da) were isolated.•Peptides showed potent ACE inhibitory activity (IC50 3.09 and 4.22μM).•Peptides protected human endothelial cells from oxidative damage.•Peptides could be used as antihypertensive agents in cardiovascular diseases.
Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829Da) and LGPLGHQ (720Da), with IC50 values of 3.09 and 4.22μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits. |
| doi_str_mv | 10.1016/j.foodchem.2013.07.067 |
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Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829Da) and LGPLGHQ (720Da), with IC50 values of 3.09 and 4.22μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2013.07.067</identifier><identifier>PMID: 24054237</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>ACE inhibition ; Amino Acid Sequence ; Angiotensin-Converting Enzyme Inhibitors - chemistry ; Angiotensin-Converting Enzyme Inhibitors - pharmacology ; Animals ; Antihypertensive Agents - chemistry ; Antihypertensive Agents - pharmacology ; Antioxidative enzymes ; Bioactive peptides ; Biological and medical sciences ; Cell Line ; Cell Survival ; Fish Proteins - chemistry ; Food toxicology ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - pharmacology ; Gelatin - chemistry ; Human endothelial ; Human Umbilical Vein Endothelial Cells - chemistry ; Human Umbilical Vein Endothelial Cells - drug effects ; Human Umbilical Vein Endothelial Cells - enzymology ; Humans ; Hydrolysis ; Kinetics ; Medical sciences ; Molecular Sequence Data ; Oxidation-Reduction ; Peptide Mapping ; Peptides - chemistry ; Peptides - isolation & purification ; Peptides - pharmacology ; Peptidyl-Dipeptidase A - chemistry ; Protein Hydrolysates - chemistry ; Protein Hydrolysates - pharmacology ; ROS ; Skate skin ; Skates (Fish) ; Skin - chemistry ; Subtilisins - chemistry ; Toxicology</subject><ispartof>Food chemistry, 2014-01, Vol.143, p.246-255</ispartof><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-3ca7e80857a19dcd5850481a641b113721ffd678d5592d364463afd0f87ba82f3</citedby><cites>FETCH-LOGICAL-c398t-3ca7e80857a19dcd5850481a641b113721ffd678d5592d364463afd0f87ba82f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814613009965$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27792797$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24054237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ngo, Dai-Hung</creatorcontrib><creatorcontrib>Ryu, BoMi</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><title>Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•Gelatin was isolated from sea food processing by-product, skate skin.•Two active peptides MVGSAPGVL (829Da) and LGPLGHQ (720Da) were isolated.•Peptides showed potent ACE inhibitory activity (IC50 3.09 and 4.22μM).•Peptides protected human endothelial cells from oxidative damage.•Peptides could be used as antihypertensive agents in cardiovascular diseases.
Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829Da) and LGPLGHQ (720Da), with IC50 values of 3.09 and 4.22μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits.</description><subject>ACE inhibition</subject><subject>Amino Acid Sequence</subject><subject>Angiotensin-Converting Enzyme Inhibitors - chemistry</subject><subject>Angiotensin-Converting Enzyme Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Antihypertensive Agents - chemistry</subject><subject>Antihypertensive Agents - pharmacology</subject><subject>Antioxidative enzymes</subject><subject>Bioactive peptides</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Fish Proteins - chemistry</subject><subject>Food toxicology</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Gelatin - chemistry</subject><subject>Human endothelial</subject><subject>Human Umbilical Vein Endothelial Cells - chemistry</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Human Umbilical Vein Endothelial Cells - enzymology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Oxidation-Reduction</subject><subject>Peptide Mapping</subject><subject>Peptides - chemistry</subject><subject>Peptides - isolation & purification</subject><subject>Peptides - pharmacology</subject><subject>Peptidyl-Dipeptidase A - chemistry</subject><subject>Protein Hydrolysates - chemistry</subject><subject>Protein Hydrolysates - pharmacology</subject><subject>ROS</subject><subject>Skate skin</subject><subject>Skates (Fish)</subject><subject>Skin - chemistry</subject><subject>Subtilisins - chemistry</subject><subject>Toxicology</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uGyEUhVHVqnHTvkLEplK6mCkMw4B3jaL-RIqUTbtGGC4O9gy4gK24D9NnLSM77bKrs-C7nHvPQeiKkpYSOnzctC5Gax5hajtCWUtESwbxAi2oFKwRRHQv0YIwIhtJ--ECvcl5QwiprHyNLrqe8L5jYoF-35jiD4B3sCveQsYuxQnnrS6Arx-2eoINeLyFEKt-qA8-4DWMulS1fvLB50esw9rHAiH70NxhE8MBUgXWGMKv4wRYzx6-HCtosQ8laQPjuB91qnYAOGnrjR6bCayvxhbHJ2-rRQxv0SunxwzvznqJfnz5_P32W3P_8PXu9ua-MWwpS8OMFiCJ5ELTpTWWS056SfXQ0xWlTHTUOTsIaTlfdpYNfT8w7SxxUqy07By7RNenf3cp_txDLmryeV5SB4j7rGjPuOw556Kiwwk1KeacwKld8pNOR0WJmrtRG_XcjZq7UUSo2k0dvDp77Ff10r9jz2VU4P0Z0LnG4ZIOxud_nBDLTixn7tOJg5rIwUNS2XgIpqaXwBRlo__fLn8Ao_Wzxg</recordid><startdate>20140115</startdate><enddate>20140115</enddate><creator>Ngo, Dai-Hung</creator><creator>Ryu, BoMi</creator><creator>Kim, Se-Kwon</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140115</creationdate><title>Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation</title><author>Ngo, Dai-Hung ; Ryu, BoMi ; Kim, Se-Kwon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-3ca7e80857a19dcd5850481a641b113721ffd678d5592d364463afd0f87ba82f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>ACE inhibition</topic><topic>Amino Acid Sequence</topic><topic>Angiotensin-Converting Enzyme Inhibitors - chemistry</topic><topic>Angiotensin-Converting Enzyme Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Antihypertensive Agents - chemistry</topic><topic>Antihypertensive Agents - pharmacology</topic><topic>Antioxidative enzymes</topic><topic>Bioactive peptides</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell Survival</topic><topic>Fish Proteins - chemistry</topic><topic>Food toxicology</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Gelatin - chemistry</topic><topic>Human endothelial</topic><topic>Human Umbilical Vein Endothelial Cells - chemistry</topic><topic>Human Umbilical Vein Endothelial Cells - drug effects</topic><topic>Human Umbilical Vein Endothelial Cells - enzymology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Oxidation-Reduction</topic><topic>Peptide Mapping</topic><topic>Peptides - chemistry</topic><topic>Peptides - isolation & purification</topic><topic>Peptides - pharmacology</topic><topic>Peptidyl-Dipeptidase A - chemistry</topic><topic>Protein Hydrolysates - chemistry</topic><topic>Protein Hydrolysates - pharmacology</topic><topic>ROS</topic><topic>Skate skin</topic><topic>Skates (Fish)</topic><topic>Skin - chemistry</topic><topic>Subtilisins - chemistry</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ngo, Dai-Hung</creatorcontrib><creatorcontrib>Ryu, BoMi</creatorcontrib><creatorcontrib>Kim, Se-Kwon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ngo, Dai-Hung</au><au>Ryu, BoMi</au><au>Kim, Se-Kwon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2014-01-15</date><risdate>2014</risdate><volume>143</volume><spage>246</spage><epage>255</epage><pages>246-255</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•Gelatin was isolated from sea food processing by-product, skate skin.•Two active peptides MVGSAPGVL (829Da) and LGPLGHQ (720Da) were isolated.•Peptides showed potent ACE inhibitory activity (IC50 3.09 and 4.22μM).•Peptides protected human endothelial cells from oxidative damage.•Peptides could be used as antihypertensive agents in cardiovascular diseases.
Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829Da) and LGPLGHQ (720Da), with IC50 values of 3.09 and 4.22μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24054237</pmid><doi>10.1016/j.foodchem.2013.07.067</doi><tpages>10</tpages></addata></record> |
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| ispartof | Food chemistry, 2014-01, Vol.143, p.246-255 |
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| subjects | ACE inhibition Amino Acid Sequence Angiotensin-Converting Enzyme Inhibitors - chemistry Angiotensin-Converting Enzyme Inhibitors - pharmacology Animals Antihypertensive Agents - chemistry Antihypertensive Agents - pharmacology Antioxidative enzymes Bioactive peptides Biological and medical sciences Cell Line Cell Survival Fish Proteins - chemistry Food toxicology Free Radical Scavengers - chemistry Free Radical Scavengers - pharmacology Gelatin - chemistry Human endothelial Human Umbilical Vein Endothelial Cells - chemistry Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - enzymology Humans Hydrolysis Kinetics Medical sciences Molecular Sequence Data Oxidation-Reduction Peptide Mapping Peptides - chemistry Peptides - isolation & purification Peptides - pharmacology Peptidyl-Dipeptidase A - chemistry Protein Hydrolysates - chemistry Protein Hydrolysates - pharmacology ROS Skate skin Skates (Fish) Skin - chemistry Subtilisins - chemistry Toxicology |
| title | Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation |
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