Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis
1 Inmunologia y Genetica Aplicada SA (INGENASA), Hermanos Garcia Noblejas 41, 28037 Madrid and 2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells pr...
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| Veröffentlicht in: | Journal of general virology 1986-01, Vol.67 (1), p.91-96 |
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| container_title | Journal of general virology |
| container_volume | 67 |
| creator | Vela, C Cambra, M Cortes, E Moreno, P Miguet, J. G De San Roman, C. Perez Sanz, A |
| description | 1 Inmunologia y Genetica Aplicada SA (INGENASA), Hermanos Garcia Noblejas 41, 28037 Madrid
and 2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.
Keywords: CTV, monoclonal antibodies, diagnosis
Received 23 April 1985;
accepted 30 September 1985. |
| doi_str_mv | 10.1099/0022-1317-67-1-91 |
| format | Article |
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and 2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.
Keywords: CTV, monoclonal antibodies, diagnosis
Received 23 April 1985;
accepted 30 September 1985.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-67-1-91</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Agronomy ; Biological and medical sciences ; Biotechnology ; citrus tristeza virus ; Fundamental and applied biological sciences. Psychology ; Industrial applications and implications. Economical aspects ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; Systematics. Structure, properties and multiplication. Genetics</subject><ispartof>Journal of general virology, 1986-01, Vol.67 (1), p.91-96</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-922e7fd93f3739dc279da1dc8f39e8adbf1491663540cd652f0d0e71cfeb566d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8714780$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Vela, C</creatorcontrib><creatorcontrib>Cambra, M</creatorcontrib><creatorcontrib>Cortes, E</creatorcontrib><creatorcontrib>Moreno, P</creatorcontrib><creatorcontrib>Miguet, J. G</creatorcontrib><creatorcontrib>De San Roman, C. Perez</creatorcontrib><creatorcontrib>Sanz, A</creatorcontrib><title>Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis</title><title>Journal of general virology</title><description>1 Inmunologia y Genetica Aplicada SA (INGENASA), Hermanos Garcia Noblejas 41, 28037 Madrid
and 2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.
Keywords: CTV, monoclonal antibodies, diagnosis
Received 23 April 1985;
accepted 30 September 1985.</description><subject>Agronomy</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>citrus tristeza virus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>Systematics. Structure, properties and multiplication. Genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpFkMtuGzEMRYWgBeqm-YDutCi6m0YczUjWMnAfCZAiAepkK8gSZTMYS640btB8fT15dUWQ9_KQuIx9BPEFhDGnQrRtAxJ0o3QDjYEjNoNO9U17UN-w2av-jr2v9U4I6Lpez9j9dclh70fKibsU-GLjivMjFnpwj8Mc-c-csh9ycgM_SyOtciCs_NcOPUXyPObCFzSWfeXLQnXEB8dvaWon4HKDVPhNxUffV3LrlCvVD-xtdEPFk-d6zG6-f1suzpvLqx8Xi7PLxncgx8a0LeoYjIxSSxN8q01wEPw8SoNzF1YROgNKyb4TPqi-jSII1OAjrnqlgjxmn5-4u5J_77GOdkvV4zC4hHlfLXSyF0rKgxGejL7kWgtGuyu0deWvBWGniO0UoZ0itEpbsAYOO5-e4a56N8Tikqf6ujjX0Om5-P_Dhtabeypo15i2dDi0omz_UHnh_QNY54sK</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Vela, C</creator><creator>Cambra, M</creator><creator>Cortes, E</creator><creator>Moreno, P</creator><creator>Miguet, J. G</creator><creator>De San Roman, C. Perez</creator><creator>Sanz, A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>19860101</creationdate><title>Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis</title><author>Vela, C ; Cambra, M ; Cortes, E ; Moreno, P ; Miguet, J. G ; De San Roman, C. Perez ; Sanz, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-922e7fd93f3739dc279da1dc8f39e8adbf1491663540cd652f0d0e71cfeb566d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Agronomy</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>citrus tristeza virus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>Systematics. Structure, properties and multiplication. Genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vela, C</creatorcontrib><creatorcontrib>Cambra, M</creatorcontrib><creatorcontrib>Cortes, E</creatorcontrib><creatorcontrib>Moreno, P</creatorcontrib><creatorcontrib>Miguet, J. G</creatorcontrib><creatorcontrib>De San Roman, C. Perez</creatorcontrib><creatorcontrib>Sanz, A</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vela, C</au><au>Cambra, M</au><au>Cortes, E</au><au>Moreno, P</au><au>Miguet, J. G</au><au>De San Roman, C. Perez</au><au>Sanz, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis</atitle><jtitle>Journal of general virology</jtitle><date>1986-01-01</date><risdate>1986</risdate><volume>67</volume><issue>1</issue><spage>91</spage><epage>96</epage><pages>91-96</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Inmunologia y Genetica Aplicada SA (INGENASA), Hermanos Garcia Noblejas 41, 28037 Madrid
and 2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.
Keywords: CTV, monoclonal antibodies, diagnosis
Received 23 April 1985;
accepted 30 September 1985.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><doi>10.1099/0022-1317-67-1-91</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1986-01, Vol.67 (1), p.91-96 |
| issn | 0022-1317 1465-2099 |
| language | eng |
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| source | Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agronomy Biological and medical sciences Biotechnology citrus tristeza virus Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids Systematics. Structure, properties and multiplication. Genetics |
| title | Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis |
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