Effect of ultrasound and high hydrostatic pressure (US/HHP) on the degradation of dextran catalyzed by dextranase
•The effect of US/HHP on enzymatic activity of dextranase was investigated.•US/HHP treatment increased the dextranase activity by 163.79% compared with the control.•Vmax, KM and kcat values of dextranase increased under US/HHP treatment compared with the control.•US/HHP treatments could be an effect...
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| Veröffentlicht in: | Ultrasonics sonochemistry 2014-01, Vol.21 (1), p.76-83 |
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| creator | Bashari, Mohanad Abdelhai, Mandour H. Abbas, Shabbar Eibaid, Ahmed Xu, Xueming Jin, Zhengyu |
| description | •The effect of US/HHP on enzymatic activity of dextranase was investigated.•US/HHP treatment increased the dextranase activity by 163.79% compared with the control.•Vmax, KM and kcat values of dextranase increased under US/HHP treatment compared with the control.•US/HHP treatments could be an effective method for improving enzyme activity in many industries.
In our current research work, the effect of combination of ultrasonic irradiation and high hydrostatic pressure (US/HHP) on the enzymatic activity and enzymatic hydrolysis kinetic parameters of dextran catalytic by dextranase were investigated. Furthermore, the effects of US/HHP on the structure of dextranase were also discussed with the aid of fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The maximum hydrolysis of dextran was observed under US (40W at 25kHz for 15min) combined with HHP (400MPa for 25min), in which the hydrolysis of dextran increased by 163.79% compared with the routine thermal incubation at 50°C. Results also showed that, Vmax and KM values, as well as, kcat of dextranase under US/HHP treatment were higher than that under US, HHP and thermal incubation at 50°C, indicated that, the substrate is converted into the product at an increased rate when compared with the incubation at 50°C. Compared to the enzymatic reaction under US, HHP, and routine thermal incubation, dextranase enzymatic reaction under US/HHP treatment showed decreases in Ea, ΔG and ΔH, however small increase in ΔS value was observed. In addition, fluorescence and CD spectra reflected that US/HHP treatment had increased the number of tryptophan on dextranase surface with increased α-helix by 19.80% and reduced random coil by 6.94% upon US/HHP-treated dextranase protein compared to the control, which were helpful for the improvement of its activity. These results indicated that, the combination of US and HHP treatments could be an effective method for improving the hydrolysis of dextran in many industrial applications including sugar manufacturing processes. |
| doi_str_mv | 10.1016/j.ultsonch.2013.04.014 |
| format | Article |
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In our current research work, the effect of combination of ultrasonic irradiation and high hydrostatic pressure (US/HHP) on the enzymatic activity and enzymatic hydrolysis kinetic parameters of dextran catalytic by dextranase were investigated. Furthermore, the effects of US/HHP on the structure of dextranase were also discussed with the aid of fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The maximum hydrolysis of dextran was observed under US (40W at 25kHz for 15min) combined with HHP (400MPa for 25min), in which the hydrolysis of dextran increased by 163.79% compared with the routine thermal incubation at 50°C. Results also showed that, Vmax and KM values, as well as, kcat of dextranase under US/HHP treatment were higher than that under US, HHP and thermal incubation at 50°C, indicated that, the substrate is converted into the product at an increased rate when compared with the incubation at 50°C. Compared to the enzymatic reaction under US, HHP, and routine thermal incubation, dextranase enzymatic reaction under US/HHP treatment showed decreases in Ea, ΔG and ΔH, however small increase in ΔS value was observed. In addition, fluorescence and CD spectra reflected that US/HHP treatment had increased the number of tryptophan on dextranase surface with increased α-helix by 19.80% and reduced random coil by 6.94% upon US/HHP-treated dextranase protein compared to the control, which were helpful for the improvement of its activity. These results indicated that, the combination of US and HHP treatments could be an effective method for improving the hydrolysis of dextran in many industrial applications including sugar manufacturing processes.</description><identifier>ISSN: 1350-4177</identifier><identifier>EISSN: 1873-2828</identifier><identifier>DOI: 10.1016/j.ultsonch.2013.04.014</identifier><identifier>PMID: 23751456</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Catalysis ; Chaetomium - enzymology ; Chemistry ; Dextran ; Dextranase ; Dextranase - chemistry ; Dextranase - metabolism ; Dextrans - chemistry ; Enzymatic activity ; Enzymatic kinetics ; Enzyme Stability ; Enzymes ; Exact sciences and technology ; General and physical chemistry ; High hydrostatic pressure ; Hydrolysis ; Hydrostatic Pressure ; Kinetics ; Physical chemistry of induced reactions (with radiations, particles and ultrasonics) ; Temperature ; Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry ; Ultrasonic chemistry ; Ultrasonics ; Ultrasound irradiation</subject><ispartof>Ultrasonics sonochemistry, 2014-01, Vol.21 (1), p.76-83</ispartof><rights>2013 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-f54faea9e6673abed8f0d72722eafaf8dcd67ddb5f6263758674e43a4fef4a9b3</citedby><cites>FETCH-LOGICAL-c398t-f54faea9e6673abed8f0d72722eafaf8dcd67ddb5f6263758674e43a4fef4a9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ultsonch.2013.04.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27844803$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23751456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bashari, Mohanad</creatorcontrib><creatorcontrib>Abdelhai, Mandour H.</creatorcontrib><creatorcontrib>Abbas, Shabbar</creatorcontrib><creatorcontrib>Eibaid, Ahmed</creatorcontrib><creatorcontrib>Xu, Xueming</creatorcontrib><creatorcontrib>Jin, Zhengyu</creatorcontrib><title>Effect of ultrasound and high hydrostatic pressure (US/HHP) on the degradation of dextran catalyzed by dextranase</title><title>Ultrasonics sonochemistry</title><addtitle>Ultrason Sonochem</addtitle><description>•The effect of US/HHP on enzymatic activity of dextranase was investigated.•US/HHP treatment increased the dextranase activity by 163.79% compared with the control.•Vmax, KM and kcat values of dextranase increased under US/HHP treatment compared with the control.•US/HHP treatments could be an effective method for improving enzyme activity in many industries.
In our current research work, the effect of combination of ultrasonic irradiation and high hydrostatic pressure (US/HHP) on the enzymatic activity and enzymatic hydrolysis kinetic parameters of dextran catalytic by dextranase were investigated. Furthermore, the effects of US/HHP on the structure of dextranase were also discussed with the aid of fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The maximum hydrolysis of dextran was observed under US (40W at 25kHz for 15min) combined with HHP (400MPa for 25min), in which the hydrolysis of dextran increased by 163.79% compared with the routine thermal incubation at 50°C. Results also showed that, Vmax and KM values, as well as, kcat of dextranase under US/HHP treatment were higher than that under US, HHP and thermal incubation at 50°C, indicated that, the substrate is converted into the product at an increased rate when compared with the incubation at 50°C. Compared to the enzymatic reaction under US, HHP, and routine thermal incubation, dextranase enzymatic reaction under US/HHP treatment showed decreases in Ea, ΔG and ΔH, however small increase in ΔS value was observed. In addition, fluorescence and CD spectra reflected that US/HHP treatment had increased the number of tryptophan on dextranase surface with increased α-helix by 19.80% and reduced random coil by 6.94% upon US/HHP-treated dextranase protein compared to the control, which were helpful for the improvement of its activity. These results indicated that, the combination of US and HHP treatments could be an effective method for improving the hydrolysis of dextran in many industrial applications including sugar manufacturing processes.</description><subject>Catalysis</subject><subject>Chaetomium - enzymology</subject><subject>Chemistry</subject><subject>Dextran</subject><subject>Dextranase</subject><subject>Dextranase - chemistry</subject><subject>Dextranase - metabolism</subject><subject>Dextrans - chemistry</subject><subject>Enzymatic activity</subject><subject>Enzymatic kinetics</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>High hydrostatic pressure</subject><subject>Hydrolysis</subject><subject>Hydrostatic Pressure</subject><subject>Kinetics</subject><subject>Physical chemistry of induced reactions (with radiations, particles and ultrasonics)</subject><subject>Temperature</subject><subject>Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry</subject><subject>Ultrasonic chemistry</subject><subject>Ultrasonics</subject><subject>Ultrasound irradiation</subject><issn>1350-4177</issn><issn>1873-2828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAURi0EoqXwFypvkMoiqV-xPTtQVRikSiBB19aNfd1klEmmdlIx_HpczQwsWVh-6NzPvseEXHJWc8b19aZehjlPo-9qwbismaoZVy_IObdGVsIK-7KsZcMqxY05I29y3jDG5Eqw1-RMSNNw1ehz8ngbI_qZTpGWwAR5WsZAoYyuf-hotw9pyjPMvae7hDkvCenV_Y_r9fr7BzqNdO6QBnxIEApT9iUn4K8SNFIPMwz73xhouz8dQsa35FWEIeO743xB7j_f_rxZV3ffvny9-XRXebmycxUbFQFhhVobCS0GG1kwwgiBECHa4IM2IbRN1EKXdqw2CpUEFTEqWLXyglwdcndpelwwz27bZ4_DACNOS3ZcSWWUaCwvqD6gvjSbE0a3S_0W0t5x5p51u4076XbPuh1TruguhZfHO5Z2i-Fv2clvAd4fAcgehlgU-D7_44xVyjJZuI8HDouRpx6Ty77H0WPoU_keF6b-f2_5A5Ojo9k</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Bashari, Mohanad</creator><creator>Abdelhai, Mandour H.</creator><creator>Abbas, Shabbar</creator><creator>Eibaid, Ahmed</creator><creator>Xu, Xueming</creator><creator>Jin, Zhengyu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201401</creationdate><title>Effect of ultrasound and high hydrostatic pressure (US/HHP) on the degradation of dextran catalyzed by dextranase</title><author>Bashari, Mohanad ; Abdelhai, Mandour H. ; Abbas, Shabbar ; Eibaid, Ahmed ; Xu, Xueming ; Jin, Zhengyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-f54faea9e6673abed8f0d72722eafaf8dcd67ddb5f6263758674e43a4fef4a9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Catalysis</topic><topic>Chaetomium - enzymology</topic><topic>Chemistry</topic><topic>Dextran</topic><topic>Dextranase</topic><topic>Dextranase - chemistry</topic><topic>Dextranase - metabolism</topic><topic>Dextrans - chemistry</topic><topic>Enzymatic activity</topic><topic>Enzymatic kinetics</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>High hydrostatic pressure</topic><topic>Hydrolysis</topic><topic>Hydrostatic Pressure</topic><topic>Kinetics</topic><topic>Physical chemistry of induced reactions (with radiations, particles and ultrasonics)</topic><topic>Temperature</topic><topic>Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry</topic><topic>Ultrasonic chemistry</topic><topic>Ultrasonics</topic><topic>Ultrasound irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bashari, Mohanad</creatorcontrib><creatorcontrib>Abdelhai, Mandour H.</creatorcontrib><creatorcontrib>Abbas, Shabbar</creatorcontrib><creatorcontrib>Eibaid, Ahmed</creatorcontrib><creatorcontrib>Xu, Xueming</creatorcontrib><creatorcontrib>Jin, Zhengyu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ultrasonics sonochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bashari, Mohanad</au><au>Abdelhai, Mandour H.</au><au>Abbas, Shabbar</au><au>Eibaid, Ahmed</au><au>Xu, Xueming</au><au>Jin, Zhengyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of ultrasound and high hydrostatic pressure (US/HHP) on the degradation of dextran catalyzed by dextranase</atitle><jtitle>Ultrasonics sonochemistry</jtitle><addtitle>Ultrason Sonochem</addtitle><date>2014-01</date><risdate>2014</risdate><volume>21</volume><issue>1</issue><spage>76</spage><epage>83</epage><pages>76-83</pages><issn>1350-4177</issn><eissn>1873-2828</eissn><abstract>•The effect of US/HHP on enzymatic activity of dextranase was investigated.•US/HHP treatment increased the dextranase activity by 163.79% compared with the control.•Vmax, KM and kcat values of dextranase increased under US/HHP treatment compared with the control.•US/HHP treatments could be an effective method for improving enzyme activity in many industries.
In our current research work, the effect of combination of ultrasonic irradiation and high hydrostatic pressure (US/HHP) on the enzymatic activity and enzymatic hydrolysis kinetic parameters of dextran catalytic by dextranase were investigated. Furthermore, the effects of US/HHP on the structure of dextranase were also discussed with the aid of fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The maximum hydrolysis of dextran was observed under US (40W at 25kHz for 15min) combined with HHP (400MPa for 25min), in which the hydrolysis of dextran increased by 163.79% compared with the routine thermal incubation at 50°C. Results also showed that, Vmax and KM values, as well as, kcat of dextranase under US/HHP treatment were higher than that under US, HHP and thermal incubation at 50°C, indicated that, the substrate is converted into the product at an increased rate when compared with the incubation at 50°C. Compared to the enzymatic reaction under US, HHP, and routine thermal incubation, dextranase enzymatic reaction under US/HHP treatment showed decreases in Ea, ΔG and ΔH, however small increase in ΔS value was observed. In addition, fluorescence and CD spectra reflected that US/HHP treatment had increased the number of tryptophan on dextranase surface with increased α-helix by 19.80% and reduced random coil by 6.94% upon US/HHP-treated dextranase protein compared to the control, which were helpful for the improvement of its activity. These results indicated that, the combination of US and HHP treatments could be an effective method for improving the hydrolysis of dextran in many industrial applications including sugar manufacturing processes.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>23751456</pmid><doi>10.1016/j.ultsonch.2013.04.014</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Catalysis Chaetomium - enzymology Chemistry Dextran Dextranase Dextranase - chemistry Dextranase - metabolism Dextrans - chemistry Enzymatic activity Enzymatic kinetics Enzyme Stability Enzymes Exact sciences and technology General and physical chemistry High hydrostatic pressure Hydrolysis Hydrostatic Pressure Kinetics Physical chemistry of induced reactions (with radiations, particles and ultrasonics) Temperature Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry Ultrasonic chemistry Ultrasonics Ultrasound irradiation |
| title | Effect of ultrasound and high hydrostatic pressure (US/HHP) on the degradation of dextran catalyzed by dextranase |
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