A New Ki-67 / E-Cadherin Cocktail Reduces Inter-observer Variation of the Calculated Proliferative Index
The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (K...
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| Veröffentlicht in: | Pathology oncology research 2013-10, Vol.19 (4), p.875-879 |
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| container_title | Pathology oncology research |
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| creator | Bubis, Golan Hilly, Ohad Bubis, Roy Halpern, Marisa Schwartz, Ariel Koren, Rumelia Rath-Wolfson, Lea |
| description | The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades. |
| doi_str_mv | 10.1007/s12253-013-9655-8 |
| format | Article |
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In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. 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Oncol. Res</addtitle><addtitle>Pathol Oncol Res</addtitle><description>The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cadherins - analysis</subject><subject>Cadherins - chemistry</subject><subject>Cadherins - metabolism</subject><subject>Cancer Research</subject><subject>Carcinoma, Ductal, Breast - chemistry</subject><subject>Carcinoma, Ductal, Breast - metabolism</subject><subject>Carcinoma, Ductal, Breast - pathology</subject><subject>Cell Growth Processes - physiology</subject><subject>Coloring Agents - chemistry</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Immunohistochemistry - standards</subject><subject>Immunology</subject><subject>Ki-67 Antigen - analysis</subject><subject>Ki-67 Antigen - chemistry</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Method</subject><subject>Neoplasm Grading</subject><subject>Observer Variation</subject><subject>Oncology</subject><subject>Pathology</subject><issn>1219-4956</issn><issn>1532-2807</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kUtLxTAQhYMovn-AGwm4cRPN47ZJllKuDxQVUbchTafeam-jSevj35tyVUQQBjIw3zkT5iC0w-gBo1QeRsZ5Jghlgug8y4haQussE5xwReVy6jnTZKKzfA1txPhIkybX-Spa40IJrjhdR7MjfAlv-LwhucSHeEoKW80gNB0uvHvqbdPiG6gGBxGfdT0E4ssI4RUCvrehsX3jO-xr3M8AF7Z1Q2t7qPB18G1TQ0jzV0jCCt630Ept2wjbX-8mujue3han5OLq5Kw4uiBOSN4TzaBitpSuhpKLsTKRKSllBTlnYLWkpdRWqFIB1KKqpXNcZ6UTmvOcUbGJ9he-z8G_DBB7M2-ig7a1HfghGjYREzlJd1MJ3fuDPvohdOl3IyW0ZkqMhmxBueBjDFCb59DMbfgwjJoxBrOIwaQYzBiDGZ13v5yHcg7Vj-L77gngCyCmUfcA4dfqf10_AWQRkNM</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Bubis, Golan</creator><creator>Hilly, Ohad</creator><creator>Bubis, Roy</creator><creator>Halpern, Marisa</creator><creator>Schwartz, Ariel</creator><creator>Koren, Rumelia</creator><creator>Rath-Wolfson, Lea</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>A New Ki-67 / E-Cadherin Cocktail Reduces Inter-observer Variation of the Calculated Proliferative Index</title><author>Bubis, Golan ; 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Oncol. Res</stitle><addtitle>Pathol Oncol Res</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>19</volume><issue>4</issue><spage>875</spage><epage>879</epage><pages>875-879</pages><issn>1219-4956</issn><eissn>1532-2807</eissn><abstract>The proliferative index in breast carcinoma is usually calculated by the percentage of the Ki-67 positive cells out of the total number of malignant cells. In order to reduce the inter-observer variability of the calculated proliferative index a cocktail of antibodies against E-Cadherin and Ki-67 (Ki/Cad Cocktail) is presented. The cocktail was applied on 59 cases of infiltrating duct carcinoma of breast and compared to the consecutive slides stained for Ki-67 alone. The Ki/Cad cocktail has the advantage that by adding the anti E-Cadherin antibody, all the malignant epithelial cells are highlighted and can be differentiated from other proliferating cells. Statistical analysis proved that the cocktail increases the inter-observer agreement from 89 % to 97 % as compared to the Ki-67 alone and also reduces the overlap between the cancer grades.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23832820</pmid><doi>10.1007/s12253-013-9655-8</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Biomedical and Life Sciences Biomedicine Breast Neoplasms - chemistry Breast Neoplasms - metabolism Breast Neoplasms - pathology Cadherins - analysis Cadherins - chemistry Cadherins - metabolism Cancer Research Carcinoma, Ductal, Breast - chemistry Carcinoma, Ductal, Breast - metabolism Carcinoma, Ductal, Breast - pathology Cell Growth Processes - physiology Coloring Agents - chemistry Female Humans Immunohistochemistry - methods Immunohistochemistry - standards Immunology Ki-67 Antigen - analysis Ki-67 Antigen - chemistry Ki-67 Antigen - metabolism Method Neoplasm Grading Observer Variation Oncology Pathology |
| title | A New Ki-67 / E-Cadherin Cocktail Reduces Inter-observer Variation of the Calculated Proliferative Index |
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