Isolation of apple leaf protoplasts

Isolation of apple leaf protoplasts

In order to make genetic manipulation accessible to fruit-trees, it was necessary to solve the problem how to isolate protoplasts. Cell wall digestion performed with commercially available, digestive enzymes was not applicable to apple leaf cells. No mixture of digestive enzymes gave more than singl...

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Veröffentlicht in:Hereditas 1985-01, Vol.3, p.no. sul.-no. sul.
Hauptverfasser: Wallin, A, Welander, M
Format: Artikel
Sprache:eng
Schlagworte:
Malus
protoplasts
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container_title Hereditas
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creator Wallin, A
Welander, M
description In order to make genetic manipulation accessible to fruit-trees, it was necessary to solve the problem how to isolate protoplasts. Cell wall digestion performed with commercially available, digestive enzymes was not applicable to apple leaf cells. No mixture of digestive enzymes gave more than single protoplasts from any part of the plant, but very often the cells were damaged. Therefore, the authors tried to change the plant tissue by altering the growth conditions of the shoots.
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source Wiley Online Library Open Access; Wiley Online Library Journals Frontfile Complete
subjects Malus
protoplasts
title Isolation of apple leaf protoplasts
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