Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice
KillerRed is known to be a unique red fluorescent protein displaying strong phototoxic properties. Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line s...
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| Veröffentlicht in: | Journal of biophotonics 2013-03, Vol.6 (3), p.283-290 |
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| creator | Shirmanova, Marina V. Serebrovskaya, Ekaterina O. Lukyanov, Konstantin A. Snopova, Ludmila B. Sirotkina, Marina A. Prodanetz, Natalia N. Bugrova, Marina L. Minakova, Ekaterina A. Turchin, Ilya V. Kamensky, Vladislav A. Lukyanov, Sergey A. Zagaynova, Elena V. |
| description | KillerRed is known to be a unique red fluorescent protein displaying strong phototoxic properties. Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line stably expressing KillerRed in mitochondria and in fusion with histone H2B was used. Irradiation of the tumors with 593 nm laser led to photobleaching of KillerRed indicating photosensitization reaction and caused significant destruction of the cells and activation of apoptosis. The portion of the dystrophically changed cells increased from 9.9% to 63.7%, and the cells with apoptosis hallmarks from 6.3% to 14%. The results of this study suggest KillerRed as a potential genetically encoded photosensitizer for photodynamic therapy of cancer. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
| doi_str_mv | 10.1002/jbio.201200056 |
| format | Article |
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Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line stably expressing KillerRed in mitochondria and in fusion with histone H2B was used. Irradiation of the tumors with 593 nm laser led to photobleaching of KillerRed indicating photosensitization reaction and caused significant destruction of the cells and activation of apoptosis. The portion of the dystrophically changed cells increased from 9.9% to 63.7%, and the cells with apoptosis hallmarks from 6.3% to 14%. The results of this study suggest KillerRed as a potential genetically encoded photosensitizer for photodynamic therapy of cancer. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</description><identifier>ISSN: 1864-063X</identifier><identifier>EISSN: 1864-0648</identifier><identifier>DOI: 10.1002/jbio.201200056</identifier><identifier>PMID: 22696211</identifier><language>eng</language><publisher>Berlin: WILEY-VCH Verlag</publisher><subject>Animals ; Cell Transformation, Neoplastic ; Chromatin - drug effects ; Chromatin - metabolism ; Chromatin - radiation effects ; Female ; GFP ; HeLa Cells ; Histones - metabolism ; Humans ; Luminescent Proteins - metabolism ; Luminescent Proteins - pharmacology ; Mice ; Mice, Nude ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondria - radiation effects ; Molecular Imaging ; pathomorphology ; Photosensitizing Agents - metabolism ; Photosensitizing Agents - pharmacology ; phototoxicity ; Protein Transport ; Red Fluorescent Protein ; RFP ; tumor xenograft</subject><ispartof>Journal of biophotonics, 2013-03, Vol.6 (3), p.283-290</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5106-c99946966bfa900020746e29acb072f2acfa7e78c33d00a7e1c518dd3682a9113</citedby><cites>FETCH-LOGICAL-c5106-c99946966bfa900020746e29acb072f2acfa7e78c33d00a7e1c518dd3682a9113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbio.201200056$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbio.201200056$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22696211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shirmanova, Marina V.</creatorcontrib><creatorcontrib>Serebrovskaya, Ekaterina O.</creatorcontrib><creatorcontrib>Lukyanov, Konstantin A.</creatorcontrib><creatorcontrib>Snopova, Ludmila B.</creatorcontrib><creatorcontrib>Sirotkina, Marina A.</creatorcontrib><creatorcontrib>Prodanetz, Natalia N.</creatorcontrib><creatorcontrib>Bugrova, Marina L.</creatorcontrib><creatorcontrib>Minakova, Ekaterina A.</creatorcontrib><creatorcontrib>Turchin, Ilya V.</creatorcontrib><creatorcontrib>Kamensky, Vladislav A.</creatorcontrib><creatorcontrib>Lukyanov, Sergey A.</creatorcontrib><creatorcontrib>Zagaynova, Elena V.</creatorcontrib><title>Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice</title><title>Journal of biophotonics</title><addtitle>J. Biophoton</addtitle><description>KillerRed is known to be a unique red fluorescent protein displaying strong phototoxic properties. Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line stably expressing KillerRed in mitochondria and in fusion with histone H2B was used. Irradiation of the tumors with 593 nm laser led to photobleaching of KillerRed indicating photosensitization reaction and caused significant destruction of the cells and activation of apoptosis. The portion of the dystrophically changed cells increased from 9.9% to 63.7%, and the cells with apoptosis hallmarks from 6.3% to 14%. The results of this study suggest KillerRed as a potential genetically encoded photosensitizer for photodynamic therapy of cancer. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</description><subject>Animals</subject><subject>Cell Transformation, Neoplastic</subject><subject>Chromatin - drug effects</subject><subject>Chromatin - metabolism</subject><subject>Chromatin - radiation effects</subject><subject>Female</subject><subject>GFP</subject><subject>HeLa Cells</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Luminescent Proteins - metabolism</subject><subject>Luminescent Proteins - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - radiation effects</subject><subject>Molecular Imaging</subject><subject>pathomorphology</subject><subject>Photosensitizing Agents - metabolism</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>phototoxicity</subject><subject>Protein Transport</subject><subject>Red Fluorescent Protein</subject><subject>RFP</subject><subject>tumor xenograft</subject><issn>1864-063X</issn><issn>1864-0648</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUha0K1BfddokssWGT4drO2PESKvoYKloQVbuzPM618JDExU5E--_r0bQjxAZ54Sv5O-ceH0KOGcwYAP-wWoY448A4AMzlDtlnjawrkHXzajuLuz1ykPMKQIKYi12yx7nUkjO2TxbXP-NYzkNwFL1HN2YaPfXdFBNmh8NI71McMQz0S-g6TN-xpXGg49THRB12XablrQ8O35DX3nYZj57vQ3Jz-vnHyXl1eXV2cfLxsnJzBrJyWuu6rJdLb3VJzUHVErm2bgmKe26dtwpV44RoAcrIiq5pWyEbbjVj4pC83_iWYL8nzKPpQ14nsQPGKRtWi3r9T9kU9N0_6CpOaSjpDBNM1ZopJgs121AuxZwTenOfQm_To2Fg1i2bdctm23IRvH22nZY9tlv8pdYC6A3wJ3T4-B87s_h0cfW3ebXRhjziw1Zr0y8jlVBzc_v1zNQLeX0q1DdzJ54AloqWow</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Shirmanova, Marina V.</creator><creator>Serebrovskaya, Ekaterina O.</creator><creator>Lukyanov, Konstantin A.</creator><creator>Snopova, Ludmila B.</creator><creator>Sirotkina, Marina A.</creator><creator>Prodanetz, Natalia N.</creator><creator>Bugrova, Marina L.</creator><creator>Minakova, Ekaterina A.</creator><creator>Turchin, Ilya V.</creator><creator>Kamensky, Vladislav A.</creator><creator>Lukyanov, Sergey A.</creator><creator>Zagaynova, Elena V.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>K9.</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>201303</creationdate><title>Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice</title><author>Shirmanova, Marina V. ; Serebrovskaya, Ekaterina O. ; Lukyanov, Konstantin A. ; Snopova, Ludmila B. ; Sirotkina, Marina A. ; Prodanetz, Natalia N. ; Bugrova, Marina L. ; Minakova, Ekaterina A. ; Turchin, Ilya V. ; Kamensky, Vladislav A. ; Lukyanov, Sergey A. ; Zagaynova, Elena V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5106-c99946966bfa900020746e29acb072f2acfa7e78c33d00a7e1c518dd3682a9113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cell Transformation, Neoplastic</topic><topic>Chromatin - drug effects</topic><topic>Chromatin - metabolism</topic><topic>Chromatin - radiation effects</topic><topic>Female</topic><topic>GFP</topic><topic>HeLa Cells</topic><topic>Histones - metabolism</topic><topic>Humans</topic><topic>Luminescent Proteins - metabolism</topic><topic>Luminescent Proteins - pharmacology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - radiation effects</topic><topic>Molecular Imaging</topic><topic>pathomorphology</topic><topic>Photosensitizing Agents - metabolism</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>phototoxicity</topic><topic>Protein Transport</topic><topic>Red Fluorescent Protein</topic><topic>RFP</topic><topic>tumor xenograft</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shirmanova, Marina V.</creatorcontrib><creatorcontrib>Serebrovskaya, Ekaterina O.</creatorcontrib><creatorcontrib>Lukyanov, Konstantin A.</creatorcontrib><creatorcontrib>Snopova, Ludmila B.</creatorcontrib><creatorcontrib>Sirotkina, Marina A.</creatorcontrib><creatorcontrib>Prodanetz, Natalia N.</creatorcontrib><creatorcontrib>Bugrova, Marina L.</creatorcontrib><creatorcontrib>Minakova, Ekaterina A.</creatorcontrib><creatorcontrib>Turchin, Ilya V.</creatorcontrib><creatorcontrib>Kamensky, Vladislav A.</creatorcontrib><creatorcontrib>Lukyanov, Sergey A.</creatorcontrib><creatorcontrib>Zagaynova, Elena V.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biophotonics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shirmanova, Marina V.</au><au>Serebrovskaya, Ekaterina O.</au><au>Lukyanov, Konstantin A.</au><au>Snopova, Ludmila B.</au><au>Sirotkina, Marina A.</au><au>Prodanetz, Natalia N.</au><au>Bugrova, Marina L.</au><au>Minakova, Ekaterina A.</au><au>Turchin, Ilya V.</au><au>Kamensky, Vladislav A.</au><au>Lukyanov, Sergey A.</au><au>Zagaynova, Elena V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice</atitle><jtitle>Journal of biophotonics</jtitle><addtitle>J. Biophoton</addtitle><date>2013-03</date><risdate>2013</risdate><volume>6</volume><issue>3</issue><spage>283</spage><epage>290</epage><pages>283-290</pages><issn>1864-063X</issn><eissn>1864-0648</eissn><abstract>KillerRed is known to be a unique red fluorescent protein displaying strong phototoxic properties. Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line stably expressing KillerRed in mitochondria and in fusion with histone H2B was used. Irradiation of the tumors with 593 nm laser led to photobleaching of KillerRed indicating photosensitization reaction and caused significant destruction of the cells and activation of apoptosis. The portion of the dystrophically changed cells increased from 9.9% to 63.7%, and the cells with apoptosis hallmarks from 6.3% to 14%. The results of this study suggest KillerRed as a potential genetically encoded photosensitizer for photodynamic therapy of cancer. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</abstract><cop>Berlin</cop><pub>WILEY-VCH Verlag</pub><pmid>22696211</pmid><doi>10.1002/jbio.201200056</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Cell Transformation, Neoplastic Chromatin - drug effects Chromatin - metabolism Chromatin - radiation effects Female GFP HeLa Cells Histones - metabolism Humans Luminescent Proteins - metabolism Luminescent Proteins - pharmacology Mice Mice, Nude Mitochondria - drug effects Mitochondria - metabolism Mitochondria - radiation effects Molecular Imaging pathomorphology Photosensitizing Agents - metabolism Photosensitizing Agents - pharmacology phototoxicity Protein Transport Red Fluorescent Protein RFP tumor xenograft |
| title | Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice |
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