Different denaturation rates between methylated and non-methylated genomic DNA can result in allele-specific PCR amplification

We analysed a DNA sample from a father and child who were both heterozygous for a 7 base pair insertion in the MEST gene differentially-methylated promoter region, previously shown by PCR analysis of bisulphite-treated DNA to be on the methylated allele in the unaffected father and the unmethylated...

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Veröffentlicht in:	Open journal of genetics 2011-09, Vol.1 (2), p.13-14
Hauptverfasser:	Bunyan, David J., Bullman, Hilary M. S., Lever, Margaret, Saminathan, Sasi D., Keng, Wee Teik, Araffin, Roziana, Robinson, David O.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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