DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India
Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial...
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| Veröffentlicht in: | Bulletin of entomological research 2013-10, Vol.103 (5), p.601-610 |
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| creator | Rebijith, K.B. Asokan, R. Kumar, N.K. Krishna Krishna, V. Chaitanya, B.N. Ramamurthy, V.V. |
| description | Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol. |
| doi_str_mv | 10.1017/S0007485313000278 |
| format | Article |
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Krishna ; Krishna, V. ; Chaitanya, B.N. ; Ramamurthy, V.V.</creator><creatorcontrib>Rebijith, K.B. ; Asokan, R. ; Kumar, N.K. Krishna ; Krishna, V. ; Chaitanya, B.N. ; Ramamurthy, V.V.</creatorcontrib><description>Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.</description><identifier>ISSN: 0007-4853</identifier><identifier>ISSN: 1475-2670</identifier><identifier>EISSN: 1475-2670</identifier><identifier>DOI: 10.1017/S0007485313000278</identifier><identifier>PMID: 23680306</identifier><identifier>CODEN: BEREA2</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animal, plant and microbial ecology ; Animals ; Aphididae ; Aphids - classification ; Aphids - genetics ; Applied ecology ; Biological and medical sciences ; Biological control ; Conservation, protection and management of environment and wildlife ; Cryptic species ; Deoxyribonucleic acid ; DNA ; DNA Barcoding, Taxonomic ; Entomology ; Fundamental and applied biological sciences. 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Krishna</creatorcontrib><creatorcontrib>Krishna, V.</creatorcontrib><creatorcontrib>Chaitanya, B.N.</creatorcontrib><creatorcontrib>Ramamurthy, V.V.</creatorcontrib><title>DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India</title><title>Bulletin of entomological research</title><addtitle>Bull. Entomol. Res</addtitle><description>Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Aphididae</subject><subject>Aphids - classification</subject><subject>Aphids - genetics</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Biological control</subject><subject>Conservation, protection and management of environment and wildlife</subject><subject>Cryptic species</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Barcoding, Taxonomic</subject><subject>Entomology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>India</subject><subject>Insects</subject><subject>Invasive insects</subject><subject>Invasive species</subject><subject>Parks, reserves, wildlife conservation. Endangered species: population survey and restocking</subject><subject>Pest control</subject><subject>Pests</subject><subject>Research Paper</subject><issn>0007-4853</issn><issn>1475-2670</issn><issn>1475-2670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0UtrFTEUAOAgir1t_QFuNFCEupg2r8mju0urtlDqotbtkEnOXFPm1WRm0X9vhnutoghdhCSc7xxOchB6S8kJJVSd3hJClNAlpzyfmNIv0IoKVRZMKvISrZZwscT30H5K9_kqjDCv0R7jUhNO5Ap9v7hZ49pGN_jQb7DtPYZ2dsHbKQw9Hhrs4uM4BYft-CN4nEZwARI-voQujBNEe4bXSyRnwEccenzV-2AP0avGtgne7PYDdPf507fzy-L665er8_V14YQkU1Fqwz1rmJI15cqVSnsnFRgAaTyrnVLcqFIoDUwLw0rdgGLZGic15brhB-h4W3eMw8MMaaq6kBy0re1hmFNFBReEaU7oMygzjJZa6EyP_qL3wxz7_JBF6TJ_nVgK0q1ycUgpQlONMXQ2PlaUVMt8qn_mk3Pe7SrPdQf-KePXQDL4sAM2Ods20fYupN9OKWGyze791jV2qOwmZnN3ywiVJC-tBcuC79qzXR2D38Afr_hvgz8BfVKrwQ</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Rebijith, K.B.</creator><creator>Asokan, R.</creator><creator>Kumar, N.K. 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Krishna</au><au>Krishna, V.</au><au>Chaitanya, B.N.</au><au>Ramamurthy, V.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India</atitle><jtitle>Bulletin of entomological research</jtitle><addtitle>Bull. Entomol. Res</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>103</volume><issue>5</issue><spage>601</spage><epage>610</epage><pages>601-610</pages><issn>0007-4853</issn><issn>1475-2670</issn><eissn>1475-2670</eissn><coden>BEREA2</coden><abstract>Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>23680306</pmid><doi>10.1017/S0007485313000278</doi><tpages>10</tpages></addata></record> |
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| ispartof | Bulletin of entomological research, 2013-10, Vol.103 (5), p.601-610 |
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| subjects | Animal, plant and microbial ecology Animals Aphididae Aphids - classification Aphids - genetics Applied ecology Biological and medical sciences Biological control Conservation, protection and management of environment and wildlife Cryptic species Deoxyribonucleic acid DNA DNA Barcoding, Taxonomic Entomology Fundamental and applied biological sciences. Psychology General aspects India Insects Invasive insects Invasive species Parks, reserves, wildlife conservation. Endangered species: population survey and restocking Pest control Pests Research Paper |
| title | DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India |
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