Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

We report the use of caprylic acid based impurity precipitation as (1) an alternative method to polishing chromatography techniques commonly used for monoclonal antibody purification and (2) an impurity reduction step prior to harvesting the bioreactor. This impurity reduction method was tested with...

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Veröffentlicht in:	Biotechnology and bioengineering 2012-10, Vol.109 (10), p.2589-2598
Hauptverfasser:	Brodsky, Yan, Zhang, Cheng, Yigzaw, Yinges, Vedantham, Ganesh
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - isolation & purification Biotechnology - methods Caprylates - chemistry caprylic acid Cell culture Chemical Precipitation Chromatography Chromatography, Affinity - methods impurity precipitation mAb process development Molecular weight Monoclonal antibodies Proteins purification Retroviridae - isolation & purification
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