Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance
Intracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high‐caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats...
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| Veröffentlicht in: | Proteomics (Weinheim) 2012-08, Vol.12 (17), p.2668-2681 |
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| creator | Berti, Denise A. Russo, Lilian C. Castro, Leandro M. Cruz, Lilian Gozzo, Fábio C. Heimann, Joel C. Lima, Fabio B. Oliveira, Ariclécio C. Andreotti, Sandra Prada, Patrícia O. Heimann, Andrea S. Ferro, Emer S. |
| description | Intracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high‐caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high‐caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin‐stimulated glucose uptake in 3T3‐L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin‐induced glucose uptake in 3T3‐L1 adipocytes. |
| doi_str_mv | 10.1002/pmic.201200051 |
| format | Article |
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The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high‐caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin‐stimulated glucose uptake in 3T3‐L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin‐induced glucose uptake in 3T3‐L1 adipocytes.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.201200051</identifier><identifier>PMID: 22740317</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>3T3 Cells ; Adipocytes - cytology ; Adipocytes - metabolism ; Adipose tissue ; Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Amino Acid Sequence ; Animals ; Biomedicine ; Chromatography, Affinity ; Chromatography, Liquid ; Energy Intake ; Glucose - metabolism ; Insulin ; Insulin - metabolism ; Insulin Resistance ; Male ; Mice ; Molecular Sequence Data ; Obesity ; Palmitic Acid - metabolism ; Peptide-protein interaction ; Peptides ; Peptides - analysis ; Peptides - metabolism ; Protein Binding ; Proteins - metabolism ; Rats ; Rats, Wistar ; Rodents ; Tandem Mass Spectrometry</subject><ispartof>Proteomics (Weinheim), 2012-08, Vol.12 (17), p.2668-2681</ispartof><rights>2012 WILEY‐VCH Verlag GmbH & Co. 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Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin‐induced glucose uptake in 3T3‐L1 adipocytes.</description><subject>3T3 Cells</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Adipose tissue</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biomedicine</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, Liquid</subject><subject>Energy Intake</subject><subject>Glucose - metabolism</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin Resistance</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Obesity</subject><subject>Palmitic Acid - metabolism</subject><subject>Peptide-protein interaction</subject><subject>Peptides</subject><subject>Peptides - analysis</subject><subject>Peptides - metabolism</subject><subject>Protein Binding</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Tandem Mass Spectrometry</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1ERUvhyhFF4tJLFo8_E25oKctKW6gECImLceIJuGSTYDuC_vc42rIHLr3YI8_vPc34EfIM6AooZS-nvW9XjAKjlEp4QM5AgSzrSsHDYy35KXkc4w2loKtaPyKnjGlBOegz8m3rcEi-861NfhyKsSv8kIJtse_n3oZiwil5hzE_F8Gmwjo_jRGL5GOc8VWxHaL__iMt_TTmI879QmL0MdmhxSfkpLN9xKd39zn5_Pby0_pdufuw2a5f78pW8FqXqqoqWzsnXVM3qqs7xRjIyuVKCMGUBLQirymV1RxAQ9O0yqFqnUUnNePn5OLgO4Xx14wxmb2PyxZ2wHGOBgQXlHGl-f0o5arSikuV0Rf_oTfjHIa8iAEuuQKlWZ2p1YFqwxhjwM5Mwe9tuM1WZonJLDGZY0xZ8PzOdm726I74v1wyIA7Ab9_j7T125vpqu9Z5lCwrD7L8-fjnKLPhp8ldLc2X9xtz_ZV-rMWbndnwv61orNw</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>Berti, Denise A.</creator><creator>Russo, Lilian C.</creator><creator>Castro, Leandro M.</creator><creator>Cruz, Lilian</creator><creator>Gozzo, Fábio C.</creator><creator>Heimann, Joel C.</creator><creator>Lima, Fabio B.</creator><creator>Oliveira, Ariclécio C.</creator><creator>Andreotti, Sandra</creator><creator>Prada, Patrícia O.</creator><creator>Heimann, Andrea S.</creator><creator>Ferro, Emer S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201208</creationdate><title>Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance</title><author>Berti, Denise A. ; 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The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high‐caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin‐stimulated glucose uptake in 3T3‐L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin‐induced glucose uptake in 3T3‐L1 adipocytes.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>22740317</pmid><doi>10.1002/pmic.201200051</doi><tpages>14</tpages></addata></record> |
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| subjects | 3T3 Cells Adipocytes - cytology Adipocytes - metabolism Adipose tissue Adipose Tissue - cytology Adipose Tissue - metabolism Amino Acid Sequence Animals Biomedicine Chromatography, Affinity Chromatography, Liquid Energy Intake Glucose - metabolism Insulin Insulin - metabolism Insulin Resistance Male Mice Molecular Sequence Data Obesity Palmitic Acid - metabolism Peptide-protein interaction Peptides Peptides - analysis Peptides - metabolism Protein Binding Proteins - metabolism Rats Rats, Wistar Rodents Tandem Mass Spectrometry |
| title | Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance |
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