Bioanalysis for Biocatalysis: Multiplexed Capillary Electrophoresis–Mass Spectrometry Assay for Aminotransferase Substrate Discovery and Specificity Profiling

In this work, we introduce an entirely automated enzyme assay based on capillary electrophoresis coupled to electrospray ionization mass spectrometry termed MINISEP-MS for multiple interfluent nanoinjections–incubation–separation–enzyme profiling using mass spectrometry. MINISEP-MS requires only nan...

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Veröffentlicht in:	Journal of the American Chemical Society 2013-09, Vol.135 (37), p.13728-13736
Hauptverfasser:	Mironov, Gleb G, St-Jacques, Antony D, Mungham, Alexander, Eason, Matthew G, Chica, Roberto A, Berezovski, Maxim V
Format:	Artikel
Sprache:	eng
Schlagworte:	Biocatalysis Biological Assay Electrophoresis, Capillary Escherichia - classification Escherichia - enzymology Mass Spectrometry Substrate Specificity Transaminases - chemistry Transaminases - metabolism
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