Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development
ABSTRACT EphAs and ephrin‐As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin‐As controls neural epithelial cell number in the developing brai...
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| Veröffentlicht in: | Developmental neurobiology (Hoboken, N.J.) N.J.), 2013-09, Vol.73 (9), p.702-712 |
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| container_title | Developmental neurobiology (Hoboken, N.J.) |
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| creator | Kim, Yujin Park, Eunjeong Noh, Hyuna Park, Soochul |
| description | ABSTRACT
EphAs and ephrin‐As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin‐As controls neural epithelial cell number in the developing brain. Ectopic expression of EphA8‐Fc in transgenic embryos induced apoptosis of neural epithelial cells, which was accompanied by a dramatic decrease in brain size. The number of ephrin‐A5‐expressing cells was significantly reduced in the brain region where EphA8‐Fc was ectopically expressed. Furthermore, in vitro culture of the dissociated neuroepithelial cells revealed that EphA8‐Fc enhanced apoptotic cell death of the ephrinA5‐expressing cells in a caspase‐dependent manner. Thus, our results suggest that reverse signaling through ephrin‐As is biochemically linked with caspase‐dependent proapoptotic signaling during early brain development. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 702–712, 2013 |
| doi_str_mv | 10.1002/dneu.22092 |
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EphAs and ephrin‐As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin‐As controls neural epithelial cell number in the developing brain. Ectopic expression of EphA8‐Fc in transgenic embryos induced apoptosis of neural epithelial cells, which was accompanied by a dramatic decrease in brain size. The number of ephrin‐A5‐expressing cells was significantly reduced in the brain region where EphA8‐Fc was ectopically expressed. Furthermore, in vitro culture of the dissociated neuroepithelial cells revealed that EphA8‐Fc enhanced apoptotic cell death of the ephrinA5‐expressing cells in a caspase‐dependent manner. Thus, our results suggest that reverse signaling through ephrin‐As is biochemically linked with caspase‐dependent proapoptotic signaling during early brain development. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 702–712, 2013</description><identifier>ISSN: 1932-8451</identifier><identifier>EISSN: 1932-846X</identifier><identifier>DOI: 10.1002/dneu.22092</identifier><identifier>PMID: 23696555</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Apoptosis ; Apoptosis - physiology ; Brain - cytology ; Brain - embryology ; Brain - metabolism ; Caspase 3 - metabolism ; Cells, Cultured ; Chromosomes, Artificial, Bacterial ; early brain development ; EphA ; Ephrin-A5 - genetics ; Ephrin-A5 - metabolism ; ephrin‐A ; Mice ; Mice, Transgenic ; Neuroepithelial Cells - metabolism ; Receptor, EphA8 - metabolism</subject><ispartof>Developmental neurobiology (Hoboken, N.J.), 2013-09, Vol.73 (9), p.702-712</ispartof><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3902-4e1d9b60eb0a0af002222e48508d2265363b3590199dd2b8e5bcef532e7a41f3</citedby><cites>FETCH-LOGICAL-c3902-4e1d9b60eb0a0af002222e48508d2265363b3590199dd2b8e5bcef532e7a41f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdneu.22092$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdneu.22092$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23696555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yujin</creatorcontrib><creatorcontrib>Park, Eunjeong</creatorcontrib><creatorcontrib>Noh, Hyuna</creatorcontrib><creatorcontrib>Park, Soochul</creatorcontrib><title>Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development</title><title>Developmental neurobiology (Hoboken, N.J.)</title><addtitle>Dev Neurobiol</addtitle><description>ABSTRACT
EphAs and ephrin‐As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin‐As controls neural epithelial cell number in the developing brain. Ectopic expression of EphA8‐Fc in transgenic embryos induced apoptosis of neural epithelial cells, which was accompanied by a dramatic decrease in brain size. The number of ephrin‐A5‐expressing cells was significantly reduced in the brain region where EphA8‐Fc was ectopically expressed. Furthermore, in vitro culture of the dissociated neuroepithelial cells revealed that EphA8‐Fc enhanced apoptotic cell death of the ephrinA5‐expressing cells in a caspase‐dependent manner. Thus, our results suggest that reverse signaling through ephrin‐As is biochemically linked with caspase‐dependent proapoptotic signaling during early brain development. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 702–712, 2013</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Brain - cytology</subject><subject>Brain - embryology</subject><subject>Brain - metabolism</subject><subject>Caspase 3 - metabolism</subject><subject>Cells, Cultured</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>early brain development</subject><subject>EphA</subject><subject>Ephrin-A5 - genetics</subject><subject>Ephrin-A5 - metabolism</subject><subject>ephrin‐A</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Neuroepithelial Cells - metabolism</subject><subject>Receptor, EphA8 - metabolism</subject><issn>1932-8451</issn><issn>1932-846X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctO3DAUhq2qqFzKpg-ALHWDkIYe23EmXqLpcJEQbKjELnLiEzBK7GAnlOmqj9Bn7JPgMJQFC1RvfCR__nR-_YR8YXDIAPg343A85BwU_0C2mBJ8VmT59cfXWbJNsh3jHYAUPIdPZJOLXOVSyi3ya_nYB4zRekd9Q5f97VHx9_ef45paR4egXbxBZ2va-TEixa4KKx_TmxlrjFT3vh98tHH6m7YIuqXY2-EWW5vGGts2UjMG625oFXRSGnzA1vcduuEz2Wh0G3H35d4hV8fLq8Xp7Pzy5GxxdD6rhQI-y5AZVeWAFWjQTQqcDmaFhMJwnkuRi0pIBUwpY3hVoKxqbFJSnOuMNWKH7K-1ffD3I8ah7GycNtMOU6iSZSIDljNZ_AfKM8GFmM8T-vUNeufH4FKOiQKATM1Fog7WVB18jAGbsg-202FVMiin7sqpu_K5uwTvvSjHqkPziv4rKwFsDfy0La7eUZXfL5Y_1tIn7c6lRg</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Kim, Yujin</creator><creator>Park, Eunjeong</creator><creator>Noh, Hyuna</creator><creator>Park, Soochul</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>201309</creationdate><title>Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development</title><author>Kim, Yujin ; Park, Eunjeong ; Noh, Hyuna ; Park, Soochul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3902-4e1d9b60eb0a0af002222e48508d2265363b3590199dd2b8e5bcef532e7a41f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Brain - cytology</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Caspase 3 - metabolism</topic><topic>Cells, Cultured</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>early brain development</topic><topic>EphA</topic><topic>Ephrin-A5 - genetics</topic><topic>Ephrin-A5 - metabolism</topic><topic>ephrin‐A</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Neuroepithelial Cells - metabolism</topic><topic>Receptor, EphA8 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yujin</creatorcontrib><creatorcontrib>Park, Eunjeong</creatorcontrib><creatorcontrib>Noh, Hyuna</creatorcontrib><creatorcontrib>Park, Soochul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Genetics Abstracts</collection><jtitle>Developmental neurobiology (Hoboken, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yujin</au><au>Park, Eunjeong</au><au>Noh, Hyuna</au><au>Park, Soochul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development</atitle><jtitle>Developmental neurobiology (Hoboken, N.J.)</jtitle><addtitle>Dev Neurobiol</addtitle><date>2013-09</date><risdate>2013</risdate><volume>73</volume><issue>9</issue><spage>702</spage><epage>712</epage><pages>702-712</pages><issn>1932-8451</issn><eissn>1932-846X</eissn><abstract>ABSTRACT
EphAs and ephrin‐As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin‐As controls neural epithelial cell number in the developing brain. Ectopic expression of EphA8‐Fc in transgenic embryos induced apoptosis of neural epithelial cells, which was accompanied by a dramatic decrease in brain size. The number of ephrin‐A5‐expressing cells was significantly reduced in the brain region where EphA8‐Fc was ectopically expressed. Furthermore, in vitro culture of the dissociated neuroepithelial cells revealed that EphA8‐Fc enhanced apoptotic cell death of the ephrinA5‐expressing cells in a caspase‐dependent manner. Thus, our results suggest that reverse signaling through ephrin‐As is biochemically linked with caspase‐dependent proapoptotic signaling during early brain development. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 702–712, 2013</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>23696555</pmid><doi>10.1002/dneu.22092</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Apoptosis Apoptosis - physiology Brain - cytology Brain - embryology Brain - metabolism Caspase 3 - metabolism Cells, Cultured Chromosomes, Artificial, Bacterial early brain development EphA Ephrin-A5 - genetics Ephrin-A5 - metabolism ephrin‐A Mice Mice, Transgenic Neuroepithelial Cells - metabolism Receptor, EphA8 - metabolism |
| title | Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development |
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