The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential
Abstract Human dental pulp stem cells (DPSCs) contain subsets of progenitor/stem cells with high angiogenic, neurogenic and regenerative potential useful for cell therapy. It is essential to develop a safe and efficacious method to isolate the clinical-grade DPSCs subsets from a small amount of pulp...
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| Veröffentlicht in: | Biomaterials 2013-12, Vol.34 (36), p.9036-9047 |
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| creator | Murakami, Masashi Horibe, Hiroshi Iohara, Koichiro Hayashi, Yuki Osako, Yohei Takei, Yoshifumi Nakata, Kazuhiko Motoyama, Noboru Kurita, Kenichi Nakashima, Misako |
| description | Abstract Human dental pulp stem cells (DPSCs) contain subsets of progenitor/stem cells with high angiogenic, neurogenic and regenerative potential useful for cell therapy. It is essential to develop a safe and efficacious method to isolate the clinical-grade DPSCs subsets from a small amount of pulp tissue without using conventional flow cytometry. Thus, a method for isolation of DPSCs subsets based on their migratory response to optimized concentration of 100 ng/ml of granulocyte-colony stimulating factor (G-CSF) was determined in this study. The DPSCs mobilized by G-CSF (MDPSCs) were enriched for CD105, C-X-C chemokine receptor type 4 (CXCR-4) and G-CSF receptor (G-CSFR) positive cells, demonstrating stem cell properties including high proliferation rate and stability. The absence of abnormalities/aberrations in karyotype and lack of tumor formation after transplantation in an immunodeficient mouse were demonstrated. The conditioned medium of MDPSCs exhibited anti-apoptotic activity, enhanced migration and immunomodulatory properties. Furthermore, transplantation of MDPSCs accelerated vasculogenesis in an ischemic hindlimb model and augmented regenerated pulp tissue in an ectopic tooth root model compared to that of colony-derived DPSCs, indicating higher regenerative potential of MDPSCs. In conclusion, this isolation method for DPSCs subsets is safe and efficacious, having utility for potential clinical applications to autologous cell transplantation. |
| doi_str_mv | 10.1016/j.biomaterials.2013.08.011 |
| format | Article |
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It is essential to develop a safe and efficacious method to isolate the clinical-grade DPSCs subsets from a small amount of pulp tissue without using conventional flow cytometry. Thus, a method for isolation of DPSCs subsets based on their migratory response to optimized concentration of 100 ng/ml of granulocyte-colony stimulating factor (G-CSF) was determined in this study. The DPSCs mobilized by G-CSF (MDPSCs) were enriched for CD105, C-X-C chemokine receptor type 4 (CXCR-4) and G-CSF receptor (G-CSFR) positive cells, demonstrating stem cell properties including high proliferation rate and stability. The absence of abnormalities/aberrations in karyotype and lack of tumor formation after transplantation in an immunodeficient mouse were demonstrated. The conditioned medium of MDPSCs exhibited anti-apoptotic activity, enhanced migration and immunomodulatory properties. Furthermore, transplantation of MDPSCs accelerated vasculogenesis in an ischemic hindlimb model and augmented regenerated pulp tissue in an ectopic tooth root model compared to that of colony-derived DPSCs, indicating higher regenerative potential of MDPSCs. In conclusion, this isolation method for DPSCs subsets is safe and efficacious, having utility for potential clinical applications to autologous cell transplantation.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2013.08.011</identifier><identifier>PMID: 23988014</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Advanced Basic Science ; Angiogenesis/vasculogenesis ; Animals ; Biomarkers - metabolism ; Carcinogenesis - pathology ; Cell Differentiation - drug effects ; Cell Lineage - drug effects ; Cell Separation - methods ; Dental Pulp - cytology ; Dental pulp stem/progenitor cells ; Dentistry ; Flow Cytometry ; Granulocyte Colony-Stimulating Factor - pharmacology ; Granulocyte-colony stimulating factor (G-CSF) ; Hematopoietic Stem Cell Mobilization ; Hindlimb - blood supply ; Hindlimb - pathology ; Humans ; Ischemia - pathology ; Mice ; Mice, SCID ; Migration ; Neovascularization, Physiologic - drug effects ; NIH 3T3 Cells ; Pulp regeneration ; Regeneration - drug effects ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stem Cell Transplantation ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism ; Tooth Root - drug effects ; Tooth Root - physiology ; Trophic effect ; Young Adult</subject><ispartof>Biomaterials, 2013-12, Vol.34 (36), p.9036-9047</ispartof><rights>The Authors</rights><rights>2013 The Authors</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-e64ddf13f3e214429245bbf7dca3f5aef905883372089f56e0711c2174ec8b773</citedby><cites>FETCH-LOGICAL-c487t-e64ddf13f3e214429245bbf7dca3f5aef905883372089f56e0711c2174ec8b773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2013.08.011$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23988014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murakami, Masashi</creatorcontrib><creatorcontrib>Horibe, Hiroshi</creatorcontrib><creatorcontrib>Iohara, Koichiro</creatorcontrib><creatorcontrib>Hayashi, Yuki</creatorcontrib><creatorcontrib>Osako, Yohei</creatorcontrib><creatorcontrib>Takei, Yoshifumi</creatorcontrib><creatorcontrib>Nakata, Kazuhiko</creatorcontrib><creatorcontrib>Motoyama, Noboru</creatorcontrib><creatorcontrib>Kurita, Kenichi</creatorcontrib><creatorcontrib>Nakashima, Misako</creatorcontrib><title>The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Human dental pulp stem cells (DPSCs) contain subsets of progenitor/stem cells with high angiogenic, neurogenic and regenerative potential useful for cell therapy. It is essential to develop a safe and efficacious method to isolate the clinical-grade DPSCs subsets from a small amount of pulp tissue without using conventional flow cytometry. Thus, a method for isolation of DPSCs subsets based on their migratory response to optimized concentration of 100 ng/ml of granulocyte-colony stimulating factor (G-CSF) was determined in this study. The DPSCs mobilized by G-CSF (MDPSCs) were enriched for CD105, C-X-C chemokine receptor type 4 (CXCR-4) and G-CSF receptor (G-CSFR) positive cells, demonstrating stem cell properties including high proliferation rate and stability. The absence of abnormalities/aberrations in karyotype and lack of tumor formation after transplantation in an immunodeficient mouse were demonstrated. The conditioned medium of MDPSCs exhibited anti-apoptotic activity, enhanced migration and immunomodulatory properties. Furthermore, transplantation of MDPSCs accelerated vasculogenesis in an ischemic hindlimb model and augmented regenerated pulp tissue in an ectopic tooth root model compared to that of colony-derived DPSCs, indicating higher regenerative potential of MDPSCs. In conclusion, this isolation method for DPSCs subsets is safe and efficacious, having utility for potential clinical applications to autologous cell transplantation.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Advanced Basic Science</subject><subject>Angiogenesis/vasculogenesis</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Carcinogenesis - pathology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Lineage - drug effects</subject><subject>Cell Separation - methods</subject><subject>Dental Pulp - cytology</subject><subject>Dental pulp stem/progenitor cells</subject><subject>Dentistry</subject><subject>Flow Cytometry</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Granulocyte-colony stimulating factor (G-CSF)</subject><subject>Hematopoietic Stem Cell Mobilization</subject><subject>Hindlimb - blood supply</subject><subject>Hindlimb - pathology</subject><subject>Humans</subject><subject>Ischemia - pathology</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Migration</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>NIH 3T3 Cells</subject><subject>Pulp regeneration</subject><subject>Regeneration - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stem Cell Transplantation</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>Tooth Root - drug effects</subject><subject>Tooth Root - physiology</subject><subject>Trophic effect</subject><subject>Young Adult</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUsuO1DAQtBCIHRZ-AVmcuCT4lYnDAQktT2klDixny3HaMx4ce7CdRcNH8M04mgUhTpysVldVd7kaoWeUtJTQ7YtDO7o46wLJaZ9bRihviWwJpffQhspeNt1AuvtoQ6hgzbCl7AI9yvlAak0Ee4guGB-krNUG_bzZA14y4GjxLumw-GhOBRoTfQwnnIubF6-LCztstSkxYRemxcCE5zg6737UXgzYro0c_bmqWhOEoj0-Lv5YRWDGBrzP-Lsre7x3uz1OsIMAqRJuAR9jqfjq5jF6YKsneHL3XqIv797eXH1orj-9_3j1-roxQvalga2YJku55cCoEGxgohtH209Gc9tpsNW_lJz3jMjBdlsgPaWG0V6AkWPf80v0_Kx7TPHbArmo2eV1RR0gLllRwTnrRcdohb48Q02KOSew6pjcrNNJUaLWPNRB_Z2HWvNQRKqaRyU_vZuzjDNMf6i_A6iAN2cAVLe3DpLKxkGoH-wSmKKm6P5vzqt_ZIx3wRntv8IJ8iEuKawcqjJTRH1eL2M9DMoJGQTj_Bc0xrtu</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Murakami, Masashi</creator><creator>Horibe, Hiroshi</creator><creator>Iohara, Koichiro</creator><creator>Hayashi, Yuki</creator><creator>Osako, Yohei</creator><creator>Takei, Yoshifumi</creator><creator>Nakata, Kazuhiko</creator><creator>Motoyama, Noboru</creator><creator>Kurita, Kenichi</creator><creator>Nakashima, Misako</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131201</creationdate><title>The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential</title><author>Murakami, Masashi ; Horibe, Hiroshi ; Iohara, Koichiro ; Hayashi, Yuki ; Osako, Yohei ; Takei, Yoshifumi ; Nakata, Kazuhiko ; Motoyama, Noboru ; Kurita, Kenichi ; Nakashima, Misako</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-e64ddf13f3e214429245bbf7dca3f5aef905883372089f56e0711c2174ec8b773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Advanced Basic Science</topic><topic>Angiogenesis/vasculogenesis</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Carcinogenesis - pathology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Lineage - drug effects</topic><topic>Cell Separation - methods</topic><topic>Dental Pulp - cytology</topic><topic>Dental pulp stem/progenitor cells</topic><topic>Dentistry</topic><topic>Flow Cytometry</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Granulocyte-colony stimulating factor (G-CSF)</topic><topic>Hematopoietic Stem Cell Mobilization</topic><topic>Hindlimb - blood supply</topic><topic>Hindlimb - pathology</topic><topic>Humans</topic><topic>Ischemia - pathology</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Migration</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>NIH 3T3 Cells</topic><topic>Pulp regeneration</topic><topic>Regeneration - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stem Cell Transplantation</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>Tooth Root - drug effects</topic><topic>Tooth Root - physiology</topic><topic>Trophic effect</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murakami, Masashi</creatorcontrib><creatorcontrib>Horibe, Hiroshi</creatorcontrib><creatorcontrib>Iohara, Koichiro</creatorcontrib><creatorcontrib>Hayashi, Yuki</creatorcontrib><creatorcontrib>Osako, Yohei</creatorcontrib><creatorcontrib>Takei, Yoshifumi</creatorcontrib><creatorcontrib>Nakata, Kazuhiko</creatorcontrib><creatorcontrib>Motoyama, Noboru</creatorcontrib><creatorcontrib>Kurita, Kenichi</creatorcontrib><creatorcontrib>Nakashima, Misako</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murakami, Masashi</au><au>Horibe, Hiroshi</au><au>Iohara, Koichiro</au><au>Hayashi, Yuki</au><au>Osako, Yohei</au><au>Takei, Yoshifumi</au><au>Nakata, Kazuhiko</au><au>Motoyama, Noboru</au><au>Kurita, Kenichi</au><au>Nakashima, Misako</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>34</volume><issue>36</issue><spage>9036</spage><epage>9047</epage><pages>9036-9047</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Human dental pulp stem cells (DPSCs) contain subsets of progenitor/stem cells with high angiogenic, neurogenic and regenerative potential useful for cell therapy. It is essential to develop a safe and efficacious method to isolate the clinical-grade DPSCs subsets from a small amount of pulp tissue without using conventional flow cytometry. Thus, a method for isolation of DPSCs subsets based on their migratory response to optimized concentration of 100 ng/ml of granulocyte-colony stimulating factor (G-CSF) was determined in this study. The DPSCs mobilized by G-CSF (MDPSCs) were enriched for CD105, C-X-C chemokine receptor type 4 (CXCR-4) and G-CSF receptor (G-CSFR) positive cells, demonstrating stem cell properties including high proliferation rate and stability. The absence of abnormalities/aberrations in karyotype and lack of tumor formation after transplantation in an immunodeficient mouse were demonstrated. The conditioned medium of MDPSCs exhibited anti-apoptotic activity, enhanced migration and immunomodulatory properties. Furthermore, transplantation of MDPSCs accelerated vasculogenesis in an ischemic hindlimb model and augmented regenerated pulp tissue in an ectopic tooth root model compared to that of colony-derived DPSCs, indicating higher regenerative potential of MDPSCs. In conclusion, this isolation method for DPSCs subsets is safe and efficacious, having utility for potential clinical applications to autologous cell transplantation.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>23988014</pmid><doi>10.1016/j.biomaterials.2013.08.011</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adolescent Adult Advanced Basic Science Angiogenesis/vasculogenesis Animals Biomarkers - metabolism Carcinogenesis - pathology Cell Differentiation - drug effects Cell Lineage - drug effects Cell Separation - methods Dental Pulp - cytology Dental pulp stem/progenitor cells Dentistry Flow Cytometry Granulocyte Colony-Stimulating Factor - pharmacology Granulocyte-colony stimulating factor (G-CSF) Hematopoietic Stem Cell Mobilization Hindlimb - blood supply Hindlimb - pathology Humans Ischemia - pathology Mice Mice, SCID Migration Neovascularization, Physiologic - drug effects NIH 3T3 Cells Pulp regeneration Regeneration - drug effects RNA, Messenger - genetics RNA, Messenger - metabolism Stem Cell Transplantation Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism Tooth Root - drug effects Tooth Root - physiology Trophic effect Young Adult |
| title | The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential |
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