Validation of high-throughput real time polymerase chain reaction assays for simultaneous detection of invasive citrus pathogens

•Total nucleic acid from tissue print, manual and high throughput extraction was compared for citrus pathogen detection.•Singleplex RT-qPCR assays to detect Hop stunt viroid and Citrus exocortis viroid were developed.•A multiplex RT-qPCR assay was developed to detect CLas, generic CTV and virulent C...

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Veröffentlicht in:	Journal of virological methods 2013-11, Vol.193 (2), p.478-486
Hauptverfasser:	Saponari, Maria, Loconsole, Giuliana, Liao, Hui-Hong, Jiang, Bo, Savino, Vito, Yokomi, Raymond K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - genetics Bacteria - isolation & purification Citrus - microbiology Citrus - virology Citrus pathogens Detection High-Throughput Screening Assays Multiplex Polymerase Chain Reaction - methods Plant Viruses - genetics Plant Viruses - isolation & purification Real time-PCR multiplex assay Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Specimen Handling - methods Viroids - genetics Viroids - isolation & purification
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