Fast Measurement of Sarcomere Length and Cell Orientation in Langendorff-Perfused Hearts Using Remote Focusing Microscopy

RATIONALE:Sarcomere length (SL) is a key indicator of cardiac mechanical function, but current imaging technologies are limited in their ability to unambiguously measure and characterize SL at the cell level in intact, living tissue. OBJECTIVE:We developed a method for measuring SL and regional cell...

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Veröffentlicht in:	Circulation research 2013-09, Vol.113 (7), p.863-870
Hauptverfasser:	Botcherby, Edward J., Corbett, Alex, Burton, Rebecca A.B., Smith, Chris W., Bollensdorff, Christian, Booth, Martin J., Kohl, Peter, Wilson, Tony, Bub, Gil
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Female Microscopy, Fluorescence, Multiphoton - methods Myocardial Reperfusion - methods Rats Rats, Sprague-Dawley Rats, Wistar Sarcomeres - ultrastructure
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