Activation of Gq Proteins Coupled with 5-HT2 Receptors in Rat Cerebral Cortical Membranes Assessed by Antibody-Capture Scintillation Proximity Assay/[35S]GTPγS Binding

Background/Aims: Functional activation of Gq coupled with 5-HT 2 receptors was investigated in rat cerebral cortical membranes. Methods: Antibody-capture scintillation proximity assay (SPA)/[ 35 S]GTPγS binding with anti-Gα q antibody was performed. Results: The specific [ 35 S]GTPγS binding to Gα q...

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Veröffentlicht in:	Pharmacology 2013-01, Vol.92 (1-2), p.2-10
Hauptverfasser:	Odagaki, Yuji, Toyoshima, Ryoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - immunology Biological Assay Carbachol - pharmacology Cerebral Cortex - metabolism GTP-Binding Protein alpha Subunits, Gq-G11 - immunology GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism Guanosine 5'-O-(3-Thiotriphosphate) - metabolism Male Original Paper Rats Rats, Sprague-Dawley Receptors, Serotonin, 5-HT2 - metabolism Serotonin - pharmacology Serotonin Antagonists - pharmacology Serotonin Receptor Agonists - pharmacology Sulfur Radioisotopes
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