Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens
A panel of 3 monoclonal antibodies (MAbs) directed against human lung tumour cell‐surface antigens has been produced following immunizations with the established small‐cell lung cancer (SCLC) cell line, NCl‐H69, and with another SCLC cell line, COR‐L32, recently derived from clinical material. One M...
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| Veröffentlicht in: | International journal of cancer 1985-06, Vol.35 (6), p.769-775 |
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| container_title | International journal of cancer |
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| creator | Reeve, Julie G. Wulfrank, Denis A. Stewart, Jonathan Twentyman, Peter R. Baillie‐Johnson, Hugo Bleehen, Norman M. |
| description | A panel of 3 monoclonal antibodies (MAbs) directed against human lung tumour cell‐surface antigens has been produced following immunizations with the established small‐cell lung cancer (SCLC) cell line, NCl‐H69, and with another SCLC cell line, COR‐L32, recently derived from clinical material. One MAb, B10/12, reacted strongly with SCLC, immunoprecipitated a protein having an MW of 100kd and failed to react significantly with non‐small‐cell lung cancer (NSCLC) in radioimmunoassay and in an immunohistochemical assay. MAbs E10/5 and 2G3 reacted extensively with SCLC but also showed significant reactivity with NSCLC. MAb E10/5 immunoprecipitated a protein with an MW of 80kd but no appreciable protein was specifically precipitated by MAb 2G3. Unlike MAb 2G3, both MAbs B10/12 and E10/5 reacted strongly with selected neuroblastomas whereas only MAbs 2G3 and E10/5 reacted significantly with melanoma. All 3 MAbs reacted with breast carcinomas. Other non‐pulmonary tumours thus far examined failed to react with the MAbs in radioimmunoassay or immunohistochemical assay. Immunocytochemistry and the use of viable cells in radioimmunoassay confirmed that the antigenic determinants recognized by these MAbs were surface located. |
| doi_str_mv | 10.1002/ijc.2910350612 |
| format | Article |
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One MAb, B10/12, reacted strongly with SCLC, immunoprecipitated a protein having an MW of 100kd and failed to react significantly with non‐small‐cell lung cancer (NSCLC) in radioimmunoassay and in an immunohistochemical assay. MAbs E10/5 and 2G3 reacted extensively with SCLC but also showed significant reactivity with NSCLC. MAb E10/5 immunoprecipitated a protein with an MW of 80kd but no appreciable protein was specifically precipitated by MAb 2G3. Unlike MAb 2G3, both MAbs B10/12 and E10/5 reacted strongly with selected neuroblastomas whereas only MAbs 2G3 and E10/5 reacted significantly with melanoma. All 3 MAbs reacted with breast carcinomas. Other non‐pulmonary tumours thus far examined failed to react with the MAbs in radioimmunoassay or immunohistochemical assay. Immunocytochemistry and the use of viable cells in radioimmunoassay confirmed that the antigenic determinants recognized by these MAbs were surface located.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910350612</identifier><identifier>PMID: 2989192</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antigens, Neoplasm - analysis ; Antigens, Surface - analysis ; Biological and medical sciences ; Carcinoma, Small Cell - immunology ; Carcinoma, Small Cell - pathology ; Cell Line ; Cross Reactions ; Female ; Histocytochemistry ; Humans ; Immunodiffusion ; Immunoenzyme Techniques ; Lung Neoplasms - immunology ; Lung Neoplasms - pathology ; Male ; Medical sciences ; Pneumology ; Precipitin Tests ; Radioimmunoassay ; Tumors of the respiratory system and mediastinum</subject><ispartof>International journal of cancer, 1985-06, Vol.35 (6), p.769-775</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3692-604684a28657837b38523979295dd01bd694743cefa76f0bfb8e89bc7659f0433</citedby><cites>FETCH-LOGICAL-c3692-604684a28657837b38523979295dd01bd694743cefa76f0bfb8e89bc7659f0433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910350612$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910350612$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9223281$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2989192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reeve, Julie G.</creatorcontrib><creatorcontrib>Wulfrank, Denis A.</creatorcontrib><creatorcontrib>Stewart, Jonathan</creatorcontrib><creatorcontrib>Twentyman, Peter R.</creatorcontrib><creatorcontrib>Baillie‐Johnson, Hugo</creatorcontrib><creatorcontrib>Bleehen, Norman M.</creatorcontrib><title>Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>A panel of 3 monoclonal antibodies (MAbs) directed against human lung tumour cell‐surface antigens has been produced following immunizations with the established small‐cell lung cancer (SCLC) cell line, NCl‐H69, and with another SCLC cell line, COR‐L32, recently derived from clinical material. One MAb, B10/12, reacted strongly with SCLC, immunoprecipitated a protein having an MW of 100kd and failed to react significantly with non‐small‐cell lung cancer (NSCLC) in radioimmunoassay and in an immunohistochemical assay. MAbs E10/5 and 2G3 reacted extensively with SCLC but also showed significant reactivity with NSCLC. MAb E10/5 immunoprecipitated a protein with an MW of 80kd but no appreciable protein was specifically precipitated by MAb 2G3. Unlike MAb 2G3, both MAbs B10/12 and E10/5 reacted strongly with selected neuroblastomas whereas only MAbs 2G3 and E10/5 reacted significantly with melanoma. All 3 MAbs reacted with breast carcinomas. Other non‐pulmonary tumours thus far examined failed to react with the MAbs in radioimmunoassay or immunohistochemical assay. Immunocytochemistry and the use of viable cells in radioimmunoassay confirmed that the antigenic determinants recognized by these MAbs were surface located.</description><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Small Cell - immunology</subject><subject>Carcinoma, Small Cell - pathology</subject><subject>Cell Line</subject><subject>Cross Reactions</subject><subject>Female</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>Immunoenzyme Techniques</subject><subject>Lung Neoplasms - immunology</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pneumology</subject><subject>Precipitin Tests</subject><subject>Radioimmunoassay</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1OwzAUhS0EKqWwsiFlQGwp13ZixyOq-CkqYoE5chy7pEqcYtdC3XgEnpEnIVGjwsZ0r3S_c-7RQegcwxQDkOtqpaZEYKApMEwO0BiD4DEQnB6icQdAzDFlx-jE-xUAxikkIzQiIhNYkDGaP7W2VXVrZf39-SXtpiractutpTaV1WX0FhppozrYZbQJTRtcpHTdsz44I5WOes1SW3-KjoysvT4b5gS93t2-zB7ixfP9fHaziBVlgsQMEpYlkmQs5RnlBc1SQgUXRKRlCbgomUh4QpU2kjMDhSkynYlCcZYKAwmlE3S181279j1ov8mbyveZpNVt8DlOKAHKSQdOd6ByrfdOm3ztqka6bY4h77vLu-7y3-46wcXgHIpGl3t8KKu7Xw536ZWsjZNWVX6PCUIoyXCHiR32UdV6-8_TfP44-xPhB2LIiWo</recordid><startdate>19850615</startdate><enddate>19850615</enddate><creator>Reeve, Julie G.</creator><creator>Wulfrank, Denis A.</creator><creator>Stewart, Jonathan</creator><creator>Twentyman, Peter R.</creator><creator>Baillie‐Johnson, Hugo</creator><creator>Bleehen, Norman M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19850615</creationdate><title>Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens</title><author>Reeve, Julie G. ; Wulfrank, Denis A. ; Stewart, Jonathan ; Twentyman, Peter R. ; Baillie‐Johnson, Hugo ; Bleehen, Norman M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3692-604684a28657837b38523979295dd01bd694743cefa76f0bfb8e89bc7659f0433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Small Cell - immunology</topic><topic>Carcinoma, Small Cell - pathology</topic><topic>Cell Line</topic><topic>Cross Reactions</topic><topic>Female</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>Immunoenzyme Techniques</topic><topic>Lung Neoplasms - immunology</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pneumology</topic><topic>Precipitin Tests</topic><topic>Radioimmunoassay</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reeve, Julie G.</creatorcontrib><creatorcontrib>Wulfrank, Denis A.</creatorcontrib><creatorcontrib>Stewart, Jonathan</creatorcontrib><creatorcontrib>Twentyman, Peter R.</creatorcontrib><creatorcontrib>Baillie‐Johnson, Hugo</creatorcontrib><creatorcontrib>Bleehen, Norman M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reeve, Julie G.</au><au>Wulfrank, Denis A.</au><au>Stewart, Jonathan</au><au>Twentyman, Peter R.</au><au>Baillie‐Johnson, Hugo</au><au>Bleehen, Norman M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1985-06-15</date><risdate>1985</risdate><volume>35</volume><issue>6</issue><spage>769</spage><epage>775</epage><pages>769-775</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>A panel of 3 monoclonal antibodies (MAbs) directed against human lung tumour cell‐surface antigens has been produced following immunizations with the established small‐cell lung cancer (SCLC) cell line, NCl‐H69, and with another SCLC cell line, COR‐L32, recently derived from clinical material. One MAb, B10/12, reacted strongly with SCLC, immunoprecipitated a protein having an MW of 100kd and failed to react significantly with non‐small‐cell lung cancer (NSCLC) in radioimmunoassay and in an immunohistochemical assay. MAbs E10/5 and 2G3 reacted extensively with SCLC but also showed significant reactivity with NSCLC. MAb E10/5 immunoprecipitated a protein with an MW of 80kd but no appreciable protein was specifically precipitated by MAb 2G3. Unlike MAb 2G3, both MAbs B10/12 and E10/5 reacted strongly with selected neuroblastomas whereas only MAbs 2G3 and E10/5 reacted significantly with melanoma. All 3 MAbs reacted with breast carcinomas. Other non‐pulmonary tumours thus far examined failed to react with the MAbs in radioimmunoassay or immunohistochemical assay. Immunocytochemistry and the use of viable cells in radioimmunoassay confirmed that the antigenic determinants recognized by these MAbs were surface located.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2989192</pmid><doi>10.1002/ijc.2910350612</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal of cancer, 1985-06, Vol.35 (6), p.769-775 |
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| subjects | Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigens, Neoplasm - analysis Antigens, Surface - analysis Biological and medical sciences Carcinoma, Small Cell - immunology Carcinoma, Small Cell - pathology Cell Line Cross Reactions Female Histocytochemistry Humans Immunodiffusion Immunoenzyme Techniques Lung Neoplasms - immunology Lung Neoplasms - pathology Male Medical sciences Pneumology Precipitin Tests Radioimmunoassay Tumors of the respiratory system and mediastinum |
| title | Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens |
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