Measuring the antioxidant capacity of blood plasma using potentiometry
The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n=60) diluted (0.3 to 1ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated c...
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| Veröffentlicht in: | Analytical biochemistry 2013-10, Vol.441 (2), p.109-114 |
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| creator | Tessutti, L.S. Macedo, D.V. Kubota, L.T. Alves, A.A. |
| description | The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n=60) diluted (0.3 to 1ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ΔE). ΔE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5−75μmol/L; R=−0.99), urate (9.0−150μmol/L; R=−0.99), and bilirubin (0.78−13μmol/L; R=−0.99). Increase in the antioxidant capacity decreased ΔE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5−50μmol/L) presented a direct correlation (0.97) with ΔE. Furthermore, ΔE presented an inverse correlation (R=−0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5−75μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n=15; exercised, n=15). This exercise decreased the concentration of urate (p |
| doi_str_mv | 10.1016/j.ab.2013.07.012 |
| format | Article |
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In this assay, plasma (n=60) diluted (0.3 to 1ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ΔE). ΔE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5−75μmol/L; R=−0.99), urate (9.0−150μmol/L; R=−0.99), and bilirubin (0.78−13μmol/L; R=−0.99). Increase in the antioxidant capacity decreased ΔE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5−50μmol/L) presented a direct correlation (0.97) with ΔE. Furthermore, ΔE presented an inverse correlation (R=−0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5−75μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n=15; exercised, n=15). This exercise decreased the concentration of urate (p<0.05), decreased FRAP (p<0.5), increased TBARS (p<0.5), and decreased the potentiometer sensor response (p=6.5×10−3). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2013.07.012</identifier><identifier>PMID: 23872008</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Animals ; antioxidant activity ; antioxidants ; Antioxidants - analysis ; Antioxidants - metabolism ; Ascorbic Acid - blood ; Ascorbic Acid - metabolism ; bilirubin ; blood plasma ; electrodes ; Exercise ; Exhaustive exercise ; Humans ; Male ; Oxidative Stress ; phosphates ; Plasma ; Plasma - metabolism ; platinum ; Potentiometry ; Potentiometry - methods ; Rats ; Rats, Wistar ; sodium chloride ; Thiobarbituric Acid Reactive Substances - analysis ; Thiobarbituric Acid Reactive Substances - metabolism ; Total antioxidant capacity ; Uric Acid - blood ; Uric Acid - metabolism ; Young Adult</subject><ispartof>Analytical biochemistry, 2013-10, Vol.441 (2), p.109-114</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-58ad6322dd77cc0376a7f7a93383393515365c92a431950c6ccb48de9b0f7e4b3</citedby><cites>FETCH-LOGICAL-c374t-58ad6322dd77cc0376a7f7a93383393515365c92a431950c6ccb48de9b0f7e4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2013.07.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23872008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tessutti, L.S.</creatorcontrib><creatorcontrib>Macedo, D.V.</creatorcontrib><creatorcontrib>Kubota, L.T.</creatorcontrib><creatorcontrib>Alves, A.A.</creatorcontrib><title>Measuring the antioxidant capacity of blood plasma using potentiometry</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n=60) diluted (0.3 to 1ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ΔE). ΔE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5−75μmol/L; R=−0.99), urate (9.0−150μmol/L; R=−0.99), and bilirubin (0.78−13μmol/L; R=−0.99). Increase in the antioxidant capacity decreased ΔE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5−50μmol/L) presented a direct correlation (0.97) with ΔE. Furthermore, ΔE presented an inverse correlation (R=−0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5−75μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n=15; exercised, n=15). This exercise decreased the concentration of urate (p<0.05), decreased FRAP (p<0.5), increased TBARS (p<0.5), and decreased the potentiometer sensor response (p=6.5×10−3). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples.</description><subject>Adolescent</subject><subject>Animals</subject><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - metabolism</subject><subject>Ascorbic Acid - blood</subject><subject>Ascorbic Acid - metabolism</subject><subject>bilirubin</subject><subject>blood plasma</subject><subject>electrodes</subject><subject>Exercise</subject><subject>Exhaustive exercise</subject><subject>Humans</subject><subject>Male</subject><subject>Oxidative Stress</subject><subject>phosphates</subject><subject>Plasma</subject><subject>Plasma - metabolism</subject><subject>platinum</subject><subject>Potentiometry</subject><subject>Potentiometry - methods</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>sodium chloride</subject><subject>Thiobarbituric Acid Reactive Substances - analysis</subject><subject>Thiobarbituric Acid Reactive Substances - metabolism</subject><subject>Total antioxidant capacity</subject><subject>Uric Acid - blood</subject><subject>Uric Acid - metabolism</subject><subject>Young Adult</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFP3TAUha2KqrzS7p0gI0vS6zixEzaESqlE1QGYrRv7hvopeQ52UvX9exw9YGM6y3fOkT7GvnEoOHD5fVtgV5TARQGqAF5-YBsOrcxBQHvENgAg8lK26ph9jnELwHlVy0_suBSNKgGaDbv-TRiX4HaP2fyXMtzNzv93NmVmcELj5n3m-6wbvLfZNGAcMVviik9-ppUeaQ77L-xjj0Okry95wh6uf9xf3eS3f37-urq8zY1Q1ZzXDVopytJapYwBoSSqXmErRCNEK2peC1mbtsRK8LYGI43pqsZS20GvqOrECTs_7E7BPy0UZz26aGgYcEd-iZqnokxCZJ1QOKAm-BgD9XoKbsSw1xz0ak9vNXZ6tadB6WQvVU5f1pduJPtWeNWVgLMD0KPX-Bhc1A93691qmkvBE3FxIChZ-Oco6Ggc7QxZF8jM2nr3_v8zFM-HSQ</recordid><startdate>20131015</startdate><enddate>20131015</enddate><creator>Tessutti, L.S.</creator><creator>Macedo, D.V.</creator><creator>Kubota, L.T.</creator><creator>Alves, A.A.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131015</creationdate><title>Measuring the antioxidant capacity of blood plasma using potentiometry</title><author>Tessutti, L.S. ; Macedo, D.V. ; Kubota, L.T. ; Alves, A.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-58ad6322dd77cc0376a7f7a93383393515365c92a431950c6ccb48de9b0f7e4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Animals</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - metabolism</topic><topic>Ascorbic Acid - blood</topic><topic>Ascorbic Acid - metabolism</topic><topic>bilirubin</topic><topic>blood plasma</topic><topic>electrodes</topic><topic>Exercise</topic><topic>Exhaustive exercise</topic><topic>Humans</topic><topic>Male</topic><topic>Oxidative Stress</topic><topic>phosphates</topic><topic>Plasma</topic><topic>Plasma - metabolism</topic><topic>platinum</topic><topic>Potentiometry</topic><topic>Potentiometry - methods</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>sodium chloride</topic><topic>Thiobarbituric Acid Reactive Substances - analysis</topic><topic>Thiobarbituric Acid Reactive Substances - metabolism</topic><topic>Total antioxidant capacity</topic><topic>Uric Acid - blood</topic><topic>Uric Acid - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tessutti, L.S.</creatorcontrib><creatorcontrib>Macedo, D.V.</creatorcontrib><creatorcontrib>Kubota, L.T.</creatorcontrib><creatorcontrib>Alves, A.A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tessutti, L.S.</au><au>Macedo, D.V.</au><au>Kubota, L.T.</au><au>Alves, A.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measuring the antioxidant capacity of blood plasma using potentiometry</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2013-10-15</date><risdate>2013</risdate><volume>441</volume><issue>2</issue><spage>109</spage><epage>114</epage><pages>109-114</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n=60) diluted (0.3 to 1ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ΔE). ΔE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5−75μmol/L; R=−0.99), urate (9.0−150μmol/L; R=−0.99), and bilirubin (0.78−13μmol/L; R=−0.99). Increase in the antioxidant capacity decreased ΔE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5−50μmol/L) presented a direct correlation (0.97) with ΔE. Furthermore, ΔE presented an inverse correlation (R=−0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5−75μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n=15; exercised, n=15). This exercise decreased the concentration of urate (p<0.05), decreased FRAP (p<0.5), increased TBARS (p<0.5), and decreased the potentiometer sensor response (p=6.5×10−3). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23872008</pmid><doi>10.1016/j.ab.2013.07.012</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Animals antioxidant activity antioxidants Antioxidants - analysis Antioxidants - metabolism Ascorbic Acid - blood Ascorbic Acid - metabolism bilirubin blood plasma electrodes Exercise Exhaustive exercise Humans Male Oxidative Stress phosphates Plasma Plasma - metabolism platinum Potentiometry Potentiometry - methods Rats Rats, Wistar sodium chloride Thiobarbituric Acid Reactive Substances - analysis Thiobarbituric Acid Reactive Substances - metabolism Total antioxidant capacity Uric Acid - blood Uric Acid - metabolism Young Adult |
| title | Measuring the antioxidant capacity of blood plasma using potentiometry |
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