A dual-template imprinted polymer-modified carbon ceramic electrode for ultra trace simultaneous analysis of ascorbic acid and dopamine
A dual-template imprinted polymer film containing dispersed multiwalled carbon nanotubes was exploited in the fabrication of a typical, reproducible, and rugged carbon ceramic electrode, adopting “surface grafting from” approach for the growth of a nanometer thin coating on its surface. For this, ch...
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creator | Bali Prasad, Bhim Jauhari, Darshika Prasad Tiwari, Mahavir |
description | A dual-template imprinted polymer film containing dispersed multiwalled carbon nanotubes was exploited in the fabrication of a typical, reproducible, and rugged carbon ceramic electrode, adopting “surface grafting from” approach for the growth of a nanometer thin coating on its surface. For this, chloro groups were first introduced at the exterior surface of silica-carbon composite electrode through sol–gel modification using (3-chloropropyl)-trimethoxysilane, followed by an iniferter (sodium diethyl dithiocarbamate) initiated photopolymerization of functional monomer (2,4,6-trisacrylamido-1,3,5-triazine), mixed templates (ascorbic acid and dopamine), and cross-linker (ethylene glycol dimethacrylate), in the presence of multiwalled carbon nanotubes. The modified sensor was validated for the simultaneous analysis of ascorbic acid and dopamine in aqueous, blood serum, cerebrospinal fluid, and pharmaceutical samples, using differential pulse anodic stripping voltammetric technique. The oxidation peak potentials for both analytes were found to be well apart approximately by 300mV, which was large enough to allow selective and sensitive analysis of one in the presence of other, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine (S/N=3). Such stringent limits could be considered suitable for the primitive diagnosis of several chronic diseases, in clinical settings.
•Synthesis of a dual-template imprinted polymer for the simultaneous analysis of AA and DA.•Polymerization done along with MWCNTs via “surface grafting from” approach at CCE.•Ultra-trace analysis LOD 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine achieved.•Selective and sensitive analysis of AA and DA in real samples containing either of the analytes. |
doi_str_mv | 10.1016/j.bios.2013.05.062 |
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•Synthesis of a dual-template imprinted polymer for the simultaneous analysis of AA and DA.•Polymerization done along with MWCNTs via “surface grafting from” approach at CCE.•Ultra-trace analysis LOD 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine achieved.•Selective and sensitive analysis of AA and DA in real samples containing either of the analytes.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2013.05.062</identifier><identifier>PMID: 23831643</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Ascorbic acid ; Ascorbic Acid - analysis ; Ascorbic Acid - blood ; Ascorbic Acid - cerebrospinal fluid ; Biological and medical sciences ; Biosensing Techniques - methods ; Biotechnology ; Carbon - chemistry ; Carbon ceramic electrode ; Ceramics - chemistry ; Differential pulse anodic stripping voltammetry ; Dopamine ; Dopamine - analysis ; Dopamine - blood ; Dopamine - cerebrospinal fluid ; Dual template imprinted polymer film ; Electrodes ; Fundamental and applied biological sciences. Psychology ; Humans ; Limit of Detection ; Molecular Imprinting ; Nanotubes, Carbon - chemistry ; Pharmaceutical Preparations - chemistry ; Polymers - chemistry ; Silicon Dioxide - chemistry ; Simultaneous ultra trace analysis</subject><ispartof>Biosensors & bioelectronics, 2013-12, Vol.50, p.19-27</ispartof><rights>2013 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-5474a09035761f3b54138a3d1ebaedf6bae3d46a19dee7b53bdb4e62a8147bc83</citedby><cites>FETCH-LOGICAL-c452t-5474a09035761f3b54138a3d1ebaedf6bae3d46a19dee7b53bdb4e62a8147bc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2013.05.062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27738731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23831643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bali Prasad, Bhim</creatorcontrib><creatorcontrib>Jauhari, Darshika</creatorcontrib><creatorcontrib>Prasad Tiwari, Mahavir</creatorcontrib><title>A dual-template imprinted polymer-modified carbon ceramic electrode for ultra trace simultaneous analysis of ascorbic acid and dopamine</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A dual-template imprinted polymer film containing dispersed multiwalled carbon nanotubes was exploited in the fabrication of a typical, reproducible, and rugged carbon ceramic electrode, adopting “surface grafting from” approach for the growth of a nanometer thin coating on its surface. For this, chloro groups were first introduced at the exterior surface of silica-carbon composite electrode through sol–gel modification using (3-chloropropyl)-trimethoxysilane, followed by an iniferter (sodium diethyl dithiocarbamate) initiated photopolymerization of functional monomer (2,4,6-trisacrylamido-1,3,5-triazine), mixed templates (ascorbic acid and dopamine), and cross-linker (ethylene glycol dimethacrylate), in the presence of multiwalled carbon nanotubes. The modified sensor was validated for the simultaneous analysis of ascorbic acid and dopamine in aqueous, blood serum, cerebrospinal fluid, and pharmaceutical samples, using differential pulse anodic stripping voltammetric technique. The oxidation peak potentials for both analytes were found to be well apart approximately by 300mV, which was large enough to allow selective and sensitive analysis of one in the presence of other, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine (S/N=3). Such stringent limits could be considered suitable for the primitive diagnosis of several chronic diseases, in clinical settings.
•Synthesis of a dual-template imprinted polymer for the simultaneous analysis of AA and DA.•Polymerization done along with MWCNTs via “surface grafting from” approach at CCE.•Ultra-trace analysis LOD 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine achieved.•Selective and sensitive analysis of AA and DA in real samples containing either of the analytes.</description><subject>Ascorbic acid</subject><subject>Ascorbic Acid - analysis</subject><subject>Ascorbic Acid - blood</subject><subject>Ascorbic Acid - cerebrospinal fluid</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biotechnology</subject><subject>Carbon - chemistry</subject><subject>Carbon ceramic electrode</subject><subject>Ceramics - chemistry</subject><subject>Differential pulse anodic stripping voltammetry</subject><subject>Dopamine</subject><subject>Dopamine - analysis</subject><subject>Dopamine - blood</subject><subject>Dopamine - cerebrospinal fluid</subject><subject>Dual template imprinted polymer film</subject><subject>Electrodes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Molecular Imprinting</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Polymers - chemistry</subject><subject>Silicon Dioxide - chemistry</subject><subject>Simultaneous ultra trace analysis</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEYhYMoTjv6Ai4kG8FNlUnlVgVuhsEbDLjRdcjlL0iTVMqkSugn8LUnTbe6c5GEJN85_JyD0GtKekqofH_sbci1HwhlPRE9kcMTdKCjYh0fmHiKDmQSshNSshv0otYjIUTRiTxHNwMbGZWcHdDvO-x3E7sN0hrNBjiktYRlA4_XHE8JSpeyD3NoD84UmxfsoJgUHIYIbivZA55zwXvcisFtOcA1pHY1C-S9YrOYeKqh4jxjU10utmmNC779eOzz2swWeImezSZWeHU9b9GPTx-_33_pHr59_np_99A5LoatE1xxQybChJJ0ZlZwykbDPAVrwM-y7cxzaejkAZQVzHrLQQ5mpFxZN7Jb9O7iu5b8c4e66RSqgxgv02rKGR0mpaahocMFdSXXWmDWLZlkyklTos8F6KM-F6DPBWgidCugid5c_XebwP-V_Em8AW-vQAvDxLmYxYX6j1OKtQZp4z5cOGhp_ApQdHUBFgc-lJa79jn8b45HjPenHA</recordid><startdate>20131215</startdate><enddate>20131215</enddate><creator>Bali Prasad, Bhim</creator><creator>Jauhari, Darshika</creator><creator>Prasad Tiwari, Mahavir</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131215</creationdate><title>A dual-template imprinted polymer-modified carbon ceramic electrode for ultra trace simultaneous analysis of ascorbic acid and dopamine</title><author>Bali Prasad, Bhim ; Jauhari, Darshika ; Prasad Tiwari, Mahavir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-5474a09035761f3b54138a3d1ebaedf6bae3d46a19dee7b53bdb4e62a8147bc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Ascorbic acid</topic><topic>Ascorbic Acid - analysis</topic><topic>Ascorbic Acid - blood</topic><topic>Ascorbic Acid - cerebrospinal fluid</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biotechnology</topic><topic>Carbon - chemistry</topic><topic>Carbon ceramic electrode</topic><topic>Ceramics - chemistry</topic><topic>Differential pulse anodic stripping voltammetry</topic><topic>Dopamine</topic><topic>Dopamine - analysis</topic><topic>Dopamine - blood</topic><topic>Dopamine - cerebrospinal fluid</topic><topic>Dual template imprinted polymer film</topic><topic>Electrodes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Molecular Imprinting</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Polymers - chemistry</topic><topic>Silicon Dioxide - chemistry</topic><topic>Simultaneous ultra trace analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bali Prasad, Bhim</creatorcontrib><creatorcontrib>Jauhari, Darshika</creatorcontrib><creatorcontrib>Prasad Tiwari, Mahavir</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bali Prasad, Bhim</au><au>Jauhari, Darshika</au><au>Prasad Tiwari, Mahavir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A dual-template imprinted polymer-modified carbon ceramic electrode for ultra trace simultaneous analysis of ascorbic acid and dopamine</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2013-12-15</date><risdate>2013</risdate><volume>50</volume><spage>19</spage><epage>27</epage><pages>19-27</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A dual-template imprinted polymer film containing dispersed multiwalled carbon nanotubes was exploited in the fabrication of a typical, reproducible, and rugged carbon ceramic electrode, adopting “surface grafting from” approach for the growth of a nanometer thin coating on its surface. For this, chloro groups were first introduced at the exterior surface of silica-carbon composite electrode through sol–gel modification using (3-chloropropyl)-trimethoxysilane, followed by an iniferter (sodium diethyl dithiocarbamate) initiated photopolymerization of functional monomer (2,4,6-trisacrylamido-1,3,5-triazine), mixed templates (ascorbic acid and dopamine), and cross-linker (ethylene glycol dimethacrylate), in the presence of multiwalled carbon nanotubes. The modified sensor was validated for the simultaneous analysis of ascorbic acid and dopamine in aqueous, blood serum, cerebrospinal fluid, and pharmaceutical samples, using differential pulse anodic stripping voltammetric technique. The oxidation peak potentials for both analytes were found to be well apart approximately by 300mV, which was large enough to allow selective and sensitive analysis of one in the presence of other, without any cross reactivity, interferences and false-positives. The detection limits realized by the proposed sensor, under optimized conditions, were found to be as low as 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine (S/N=3). Such stringent limits could be considered suitable for the primitive diagnosis of several chronic diseases, in clinical settings.
•Synthesis of a dual-template imprinted polymer for the simultaneous analysis of AA and DA.•Polymerization done along with MWCNTs via “surface grafting from” approach at CCE.•Ultra-trace analysis LOD 2.24ngmL−1 for ascorbic acid and 0.21ngmL−1 for dopamine achieved.•Selective and sensitive analysis of AA and DA in real samples containing either of the analytes.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>23831643</pmid><doi>10.1016/j.bios.2013.05.062</doi><tpages>9</tpages></addata></record> |
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subjects | Ascorbic acid Ascorbic Acid - analysis Ascorbic Acid - blood Ascorbic Acid - cerebrospinal fluid Biological and medical sciences Biosensing Techniques - methods Biotechnology Carbon - chemistry Carbon ceramic electrode Ceramics - chemistry Differential pulse anodic stripping voltammetry Dopamine Dopamine - analysis Dopamine - blood Dopamine - cerebrospinal fluid Dual template imprinted polymer film Electrodes Fundamental and applied biological sciences. Psychology Humans Limit of Detection Molecular Imprinting Nanotubes, Carbon - chemistry Pharmaceutical Preparations - chemistry Polymers - chemistry Silicon Dioxide - chemistry Simultaneous ultra trace analysis |
title | A dual-template imprinted polymer-modified carbon ceramic electrode for ultra trace simultaneous analysis of ascorbic acid and dopamine |
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