HPV-16 exposed mouse embryos: a potential model for pregnancy wastage
Purpose Placentas from spontaneous abortions and preterm deliveries have a higher prevalence of Human papillomavirus (HPV) compared to placentas from elective abortions and term births. The objective was to determine the effects of HPV-16 on the adhesion and implantation properties of early embryo t...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2013-06, Vol.287 (6), p.1093-1097 |
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| container_title | Archives of gynecology and obstetrics |
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| creator | Hong, Linda J. Oshiro, Bryan T. Chan, Philip J. |
| description | Purpose
Placentas from spontaneous abortions and preterm deliveries have a higher prevalence of Human papillomavirus (HPV) compared to placentas from elective abortions and term births. The objective was to determine the effects of HPV-16 on the adhesion and implantation properties of early embryo trophoblasts.
Methods
Two-cell mouse embryos were cultured (medium G2, 5 % CO
2
, 37 °C) for 72–96 h and exposed to either HPV-16 rich SiHa cell lysates which were refrigerated after mechanical lysis, thawed lysates which had been frozen for freeze/thaw lysis method, or control medium, incubated (4–5 days) and evaluated by microscopy (
N
= 96 embryos, 3 repeated experiments). Trophoblasts were stained and images were digitized. Adhesion and dimension data were analyzed by Chi-square and
t
test, respectively.
Results
HPV-16 exposed embryos exhibited less adhesion through reduced implantation compared with the control (combined lysates 53.8 vs. 85.7 %,
P
|
| doi_str_mv | 10.1007/s00404-013-2711-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1430860951</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2261875091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-8098641deb51c256f21461b6d32386b993a9b5c26f6e07fb8a5112ae92477e1d3</originalsourceid><addsrcrecordid>eNqFkU1LxDAQhoMo7vrxA7xIwYuX6kzSpI03Eb9gQQ_qNaTtdFnpl0mL7r83y64KgngIkzDPvJOZl7EjhDMESM89QAJJDChiniLGcotNMRE8hvDaZlPQqzuodML2vH8FQJ5lapdNuBABUemUXd89vsSoIvroO09l1HSjp4ia3C07fxHZqO8GaoeFrUOqpDqqOhf1juatbYtl9G79YOd0wHYqW3s63MR99nxz_XR1F88ebu-vLmdxkYAc4gx0phIsKZdYcKkqjonCXJWCi0zlWgurc1lwVSmCtMozKxG5Jc2TNCUsxT47Xev2rnsbyQ-mWfiC6tq2FD5uwvCQKdAS_0eFFCB0OAE9-YW-dqNrwyCGc4VZKkGvBHFNFa7z3lFlerdorFsaBLOyw6ztMMEOs7LDyFBzvFEe84bK74qv_QeArwEfUu2c3E_rv1U_AXKokXI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2261875091</pqid></control><display><type>article</type><title>HPV-16 exposed mouse embryos: a potential model for pregnancy wastage</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Hong, Linda J. ; Oshiro, Bryan T. ; Chan, Philip J.</creator><creatorcontrib>Hong, Linda J. ; Oshiro, Bryan T. ; Chan, Philip J.</creatorcontrib><description>Purpose
Placentas from spontaneous abortions and preterm deliveries have a higher prevalence of Human papillomavirus (HPV) compared to placentas from elective abortions and term births. The objective was to determine the effects of HPV-16 on the adhesion and implantation properties of early embryo trophoblasts.
Methods
Two-cell mouse embryos were cultured (medium G2, 5 % CO
2
, 37 °C) for 72–96 h and exposed to either HPV-16 rich SiHa cell lysates which were refrigerated after mechanical lysis, thawed lysates which had been frozen for freeze/thaw lysis method, or control medium, incubated (4–5 days) and evaluated by microscopy (
N
= 96 embryos, 3 repeated experiments). Trophoblasts were stained and images were digitized. Adhesion and dimension data were analyzed by Chi-square and
t
test, respectively.
Results
HPV-16 exposed embryos exhibited less adhesion through reduced implantation compared with the control (combined lysates 53.8 vs. 85.7 %,
P
< 0.05). Refrigerated and thawed lysate groups had similar reduced implantations (58.3 vs. 50.0 %). Of the embryos with implantation, 100 % in the refrigerated lysates were noted to have loose or abnormal adhesion. This was measured when embryos were noted to be lost after washes with HTF. There was no difference in trophoblast viability among the groups. Total trophoblast area was greater in the HPV-16 exposed frozen lysate group (1,881.8 ± 605.3 vs. control 848.8 ± 298.0 square units, mean ± SEM).
Conclusions
HPV-16 inhibited trophoblasts adhesion needed for normal implantation, but not embryo development. Total trophoblast spread was increased after HPV-16 exposure suggesting that HPV-16 altered trophoblast migration. These results suggest that HPV-16 may induce abnormal placental growth resulting in pregnancy wastage.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-013-2711-5</identifier><identifier>PMID: 23307167</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Abortion, Spontaneous - virology ; Animals ; Embryo Culture Techniques ; Embryo Implantation ; Embryo Transfer ; Embryo, Mammalian - virology ; Embryonic Development ; Embryos ; Endocrinology ; Female ; Gynecology ; Human Genetics ; Human papillomavirus ; Human papillomavirus 16 - physiology ; Maternal-Fetal Medicine ; Medicine ; Medicine & Public Health ; Mice ; Obstetrics/Perinatology/Midwifery ; Pregnancy ; Trophoblasts - physiology ; Trophoblasts - virology</subject><ispartof>Archives of gynecology and obstetrics, 2013-06, Vol.287 (6), p.1093-1097</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>Archives of Gynecology and Obstetrics is a copyright of Springer, (2013). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-8098641deb51c256f21461b6d32386b993a9b5c26f6e07fb8a5112ae92477e1d3</citedby><cites>FETCH-LOGICAL-c405t-8098641deb51c256f21461b6d32386b993a9b5c26f6e07fb8a5112ae92477e1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-013-2711-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-013-2711-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23307167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, Linda J.</creatorcontrib><creatorcontrib>Oshiro, Bryan T.</creatorcontrib><creatorcontrib>Chan, Philip J.</creatorcontrib><title>HPV-16 exposed mouse embryos: a potential model for pregnancy wastage</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
Placentas from spontaneous abortions and preterm deliveries have a higher prevalence of Human papillomavirus (HPV) compared to placentas from elective abortions and term births. The objective was to determine the effects of HPV-16 on the adhesion and implantation properties of early embryo trophoblasts.
Methods
Two-cell mouse embryos were cultured (medium G2, 5 % CO
2
, 37 °C) for 72–96 h and exposed to either HPV-16 rich SiHa cell lysates which were refrigerated after mechanical lysis, thawed lysates which had been frozen for freeze/thaw lysis method, or control medium, incubated (4–5 days) and evaluated by microscopy (
N
= 96 embryos, 3 repeated experiments). Trophoblasts were stained and images were digitized. Adhesion and dimension data were analyzed by Chi-square and
t
test, respectively.
Results
HPV-16 exposed embryos exhibited less adhesion through reduced implantation compared with the control (combined lysates 53.8 vs. 85.7 %,
P
< 0.05). Refrigerated and thawed lysate groups had similar reduced implantations (58.3 vs. 50.0 %). Of the embryos with implantation, 100 % in the refrigerated lysates were noted to have loose or abnormal adhesion. This was measured when embryos were noted to be lost after washes with HTF. There was no difference in trophoblast viability among the groups. Total trophoblast area was greater in the HPV-16 exposed frozen lysate group (1,881.8 ± 605.3 vs. control 848.8 ± 298.0 square units, mean ± SEM).
Conclusions
HPV-16 inhibited trophoblasts adhesion needed for normal implantation, but not embryo development. Total trophoblast spread was increased after HPV-16 exposure suggesting that HPV-16 altered trophoblast migration. These results suggest that HPV-16 may induce abnormal placental growth resulting in pregnancy wastage.</description><subject>Abortion, Spontaneous - virology</subject><subject>Animals</subject><subject>Embryo Culture Techniques</subject><subject>Embryo Implantation</subject><subject>Embryo Transfer</subject><subject>Embryo, Mammalian - virology</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Endocrinology</subject><subject>Female</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 16 - physiology</subject><subject>Maternal-Fetal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Pregnancy</subject><subject>Trophoblasts - physiology</subject><subject>Trophoblasts - virology</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1LxDAQhoMo7vrxA7xIwYuX6kzSpI03Eb9gQQ_qNaTtdFnpl0mL7r83y64KgngIkzDPvJOZl7EjhDMESM89QAJJDChiniLGcotNMRE8hvDaZlPQqzuodML2vH8FQJ5lapdNuBABUemUXd89vsSoIvroO09l1HSjp4ia3C07fxHZqO8GaoeFrUOqpDqqOhf1juatbYtl9G79YOd0wHYqW3s63MR99nxz_XR1F88ebu-vLmdxkYAc4gx0phIsKZdYcKkqjonCXJWCi0zlWgurc1lwVSmCtMozKxG5Jc2TNCUsxT47Xev2rnsbyQ-mWfiC6tq2FD5uwvCQKdAS_0eFFCB0OAE9-YW-dqNrwyCGc4VZKkGvBHFNFa7z3lFlerdorFsaBLOyw6ztMMEOs7LDyFBzvFEe84bK74qv_QeArwEfUu2c3E_rv1U_AXKokXI</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Hong, Linda J.</creator><creator>Oshiro, Bryan T.</creator><creator>Chan, Philip J.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20130601</creationdate><title>HPV-16 exposed mouse embryos: a potential model for pregnancy wastage</title><author>Hong, Linda J. ; Oshiro, Bryan T. ; Chan, Philip J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-8098641deb51c256f21461b6d32386b993a9b5c26f6e07fb8a5112ae92477e1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abortion, Spontaneous - virology</topic><topic>Animals</topic><topic>Embryo Culture Techniques</topic><topic>Embryo Implantation</topic><topic>Embryo Transfer</topic><topic>Embryo, Mammalian - virology</topic><topic>Embryonic Development</topic><topic>Embryos</topic><topic>Endocrinology</topic><topic>Female</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Human papillomavirus</topic><topic>Human papillomavirus 16 - physiology</topic><topic>Maternal-Fetal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Pregnancy</topic><topic>Trophoblasts - physiology</topic><topic>Trophoblasts - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, Linda J.</creatorcontrib><creatorcontrib>Oshiro, Bryan T.</creatorcontrib><creatorcontrib>Chan, Philip J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Linda J.</au><au>Oshiro, Bryan T.</au><au>Chan, Philip J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPV-16 exposed mouse embryos: a potential model for pregnancy wastage</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>287</volume><issue>6</issue><spage>1093</spage><epage>1097</epage><pages>1093-1097</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
Placentas from spontaneous abortions and preterm deliveries have a higher prevalence of Human papillomavirus (HPV) compared to placentas from elective abortions and term births. The objective was to determine the effects of HPV-16 on the adhesion and implantation properties of early embryo trophoblasts.
Methods
Two-cell mouse embryos were cultured (medium G2, 5 % CO
2
, 37 °C) for 72–96 h and exposed to either HPV-16 rich SiHa cell lysates which were refrigerated after mechanical lysis, thawed lysates which had been frozen for freeze/thaw lysis method, or control medium, incubated (4–5 days) and evaluated by microscopy (
N
= 96 embryos, 3 repeated experiments). Trophoblasts were stained and images were digitized. Adhesion and dimension data were analyzed by Chi-square and
t
test, respectively.
Results
HPV-16 exposed embryos exhibited less adhesion through reduced implantation compared with the control (combined lysates 53.8 vs. 85.7 %,
P
< 0.05). Refrigerated and thawed lysate groups had similar reduced implantations (58.3 vs. 50.0 %). Of the embryos with implantation, 100 % in the refrigerated lysates were noted to have loose or abnormal adhesion. This was measured when embryos were noted to be lost after washes with HTF. There was no difference in trophoblast viability among the groups. Total trophoblast area was greater in the HPV-16 exposed frozen lysate group (1,881.8 ± 605.3 vs. control 848.8 ± 298.0 square units, mean ± SEM).
Conclusions
HPV-16 inhibited trophoblasts adhesion needed for normal implantation, but not embryo development. Total trophoblast spread was increased after HPV-16 exposure suggesting that HPV-16 altered trophoblast migration. These results suggest that HPV-16 may induce abnormal placental growth resulting in pregnancy wastage.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23307167</pmid><doi>10.1007/s00404-013-2711-5</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0067 |
| ispartof | Archives of gynecology and obstetrics, 2013-06, Vol.287 (6), p.1093-1097 |
| issn | 0932-0067 1432-0711 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1430860951 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abortion, Spontaneous - virology Animals Embryo Culture Techniques Embryo Implantation Embryo Transfer Embryo, Mammalian - virology Embryonic Development Embryos Endocrinology Female Gynecology Human Genetics Human papillomavirus Human papillomavirus 16 - physiology Maternal-Fetal Medicine Medicine Medicine & Public Health Mice Obstetrics/Perinatology/Midwifery Pregnancy Trophoblasts - physiology Trophoblasts - virology |
| title | HPV-16 exposed mouse embryos: a potential model for pregnancy wastage |
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