Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors
A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables. Salmonella typhimurium was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions. S. typhimurium was inoculated o...
Gespeichert in:
| Veröffentlicht in: | Food and bioprocess technology 2013-03, Vol.6 (3), p.682-689 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 689 |
|---|---|
| container_issue | 3 |
| container_start_page | 682 |
| container_title | Food and bioprocess technology |
| container_volume | 6 |
| creator | Park, Mi-Kyung Li, Suiqiong Chin, Bryan A. |
| description | A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables.
Salmonella typhimurium
was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions.
S. typhimurium
was inoculated on the surface of fresh tomatoes, and ME biosensors were used to detect the bacteria. The populations of
S. typhimurium
after 24-h incubation time at 37°C and 100% relative humidity were 6.1 and 7.8 logCFU/cm
2
after starting with initial inoculations of 3.0 and 5.0 logCFU/cm
2
, respectively. After evident growth, measurement sensors with E2 phage immobilized on their surfaces and control sensors devoid of E2 phage were placed on the inoculated tomato surfaces and the resonant frequency shifts were measured. As the population of
S. typhimurium
increased, the resonant frequency shifts of the measurement sensors significantly increased (
P
|
| doi_str_mv | 10.1007/s11947-011-0708-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1430858981</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1430858981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-50dd7c31bd1851f82a143a8178b4aeb14e96f07958212d2881a329d7d0b49e93</originalsourceid><addsrcrecordid>eNp1kU1LxDAQhosoqKs_wFvAi5dqJkk3ydFvBUXB9Ryy7XSNtMmatMj-e7OsKAieZg7P-zLDUxRHQE-BUnmWALSQJQUoqaSqZFvFHmhelRUIvf2zc7pb7Kf0TumUCuB7xfIKB6wHFzwJLXmxXR88dp0lw2r55voxurEntzF8enLlYia7FcnsLPR2CORljK2tkbwm5xfk-c0usLywCRvyaBceh4CdTYOryYULCX0KMR0UO63tEh5-z0kxu7meXd6VD0-395fnD2XNhR7KijaNrDnMG1AVtIpZENwqkGouLM5BoJ62VOpKMWANUwosZ7qRDZ0LjZpPipNN7TKGjxHTYHqX6vVnHsOYTG6jqlJaQUaP_6DvYYw-H2eYACql5FpkCjZUHUNKEVuzjK63cWWAmrUCs1FgsgKzVmBYzrBNJmXWLzD-Nv8f-gIy6Ik9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2410777394</pqid></control><display><type>article</type><title>Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors</title><source>SpringerLink Journals - AutoHoldings</source><creator>Park, Mi-Kyung ; Li, Suiqiong ; Chin, Bryan A.</creator><creatorcontrib>Park, Mi-Kyung ; Li, Suiqiong ; Chin, Bryan A.</creatorcontrib><description>A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables.
Salmonella typhimurium
was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions.
S. typhimurium
was inoculated on the surface of fresh tomatoes, and ME biosensors were used to detect the bacteria. The populations of
S. typhimurium
after 24-h incubation time at 37°C and 100% relative humidity were 6.1 and 7.8 logCFU/cm
2
after starting with initial inoculations of 3.0 and 5.0 logCFU/cm
2
, respectively. After evident growth, measurement sensors with E2 phage immobilized on their surfaces and control sensors devoid of E2 phage were placed on the inoculated tomato surfaces and the resonant frequency shifts were measured. As the population of
S. typhimurium
increased, the resonant frequency shifts of the measurement sensors significantly increased (
P
< 0.01), exhibiting 6,680 ± 665 and 9,384 ± 457 Hz for the populations of 6.1 and 7.8 logCFU/cm
2
, respectively. SEM images confirmed that the measurement sensor resonant frequency shifts were due to
S. typhimurium
binding with E2 phage. This study demonstrated that the ME biosensor method could detect
S. typhimurium
grown directly on tomato surfaces with limited sample preparation procedures. Therefore, the ME biosensor could be applied as a cost and time effective, relatively simple, practically suitable on-site detection method for
S. typhimurium
in fresh produce.</description><identifier>ISSN: 1935-5130</identifier><identifier>EISSN: 1935-5149</identifier><identifier>DOI: 10.1007/s11947-011-0708-2</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Agriculture ; Biosensors ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Environmental conditions ; Food Science ; Inoculation ; Lycopersicon esculentum ; Nutrients ; Original Paper ; Phages ; Populations ; Relative humidity ; Resonant frequencies ; Salmonella ; Sample preparation ; Sensors ; Tomatoes</subject><ispartof>Food and bioprocess technology, 2013-03, Vol.6 (3), p.682-689</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>Springer Science+Business Media, LLC 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-50dd7c31bd1851f82a143a8178b4aeb14e96f07958212d2881a329d7d0b49e93</citedby><cites>FETCH-LOGICAL-c349t-50dd7c31bd1851f82a143a8178b4aeb14e96f07958212d2881a329d7d0b49e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11947-011-0708-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11947-011-0708-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Park, Mi-Kyung</creatorcontrib><creatorcontrib>Li, Suiqiong</creatorcontrib><creatorcontrib>Chin, Bryan A.</creatorcontrib><title>Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors</title><title>Food and bioprocess technology</title><addtitle>Food Bioprocess Technol</addtitle><description>A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables.
Salmonella typhimurium
was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions.
S. typhimurium
was inoculated on the surface of fresh tomatoes, and ME biosensors were used to detect the bacteria. The populations of
S. typhimurium
after 24-h incubation time at 37°C and 100% relative humidity were 6.1 and 7.8 logCFU/cm
2
after starting with initial inoculations of 3.0 and 5.0 logCFU/cm
2
, respectively. After evident growth, measurement sensors with E2 phage immobilized on their surfaces and control sensors devoid of E2 phage were placed on the inoculated tomato surfaces and the resonant frequency shifts were measured. As the population of
S. typhimurium
increased, the resonant frequency shifts of the measurement sensors significantly increased (
P
< 0.01), exhibiting 6,680 ± 665 and 9,384 ± 457 Hz for the populations of 6.1 and 7.8 logCFU/cm
2
, respectively. SEM images confirmed that the measurement sensor resonant frequency shifts were due to
S. typhimurium
binding with E2 phage. This study demonstrated that the ME biosensor method could detect
S. typhimurium
grown directly on tomato surfaces with limited sample preparation procedures. Therefore, the ME biosensor could be applied as a cost and time effective, relatively simple, practically suitable on-site detection method for
S. typhimurium
in fresh produce.</description><subject>Agriculture</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Environmental conditions</subject><subject>Food Science</subject><subject>Inoculation</subject><subject>Lycopersicon esculentum</subject><subject>Nutrients</subject><subject>Original Paper</subject><subject>Phages</subject><subject>Populations</subject><subject>Relative humidity</subject><subject>Resonant frequencies</subject><subject>Salmonella</subject><subject>Sample preparation</subject><subject>Sensors</subject><subject>Tomatoes</subject><issn>1935-5130</issn><issn>1935-5149</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kU1LxDAQhosoqKs_wFvAi5dqJkk3ydFvBUXB9Ryy7XSNtMmatMj-e7OsKAieZg7P-zLDUxRHQE-BUnmWALSQJQUoqaSqZFvFHmhelRUIvf2zc7pb7Kf0TumUCuB7xfIKB6wHFzwJLXmxXR88dp0lw2r55voxurEntzF8enLlYia7FcnsLPR2CORljK2tkbwm5xfk-c0usLywCRvyaBceh4CdTYOryYULCX0KMR0UO63tEh5-z0kxu7meXd6VD0-395fnD2XNhR7KijaNrDnMG1AVtIpZENwqkGouLM5BoJ62VOpKMWANUwosZ7qRDZ0LjZpPipNN7TKGjxHTYHqX6vVnHsOYTG6jqlJaQUaP_6DvYYw-H2eYACql5FpkCjZUHUNKEVuzjK63cWWAmrUCs1FgsgKzVmBYzrBNJmXWLzD-Nv8f-gIy6Ik9</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Park, Mi-Kyung</creator><creator>Li, Suiqiong</creator><creator>Chin, Bryan A.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130301</creationdate><title>Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors</title><author>Park, Mi-Kyung ; Li, Suiqiong ; Chin, Bryan A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-50dd7c31bd1851f82a143a8178b4aeb14e96f07958212d2881a329d7d0b49e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agriculture</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Environmental conditions</topic><topic>Food Science</topic><topic>Inoculation</topic><topic>Lycopersicon esculentum</topic><topic>Nutrients</topic><topic>Original Paper</topic><topic>Phages</topic><topic>Populations</topic><topic>Relative humidity</topic><topic>Resonant frequencies</topic><topic>Salmonella</topic><topic>Sample preparation</topic><topic>Sensors</topic><topic>Tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Mi-Kyung</creatorcontrib><creatorcontrib>Li, Suiqiong</creatorcontrib><creatorcontrib>Chin, Bryan A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food and bioprocess technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Mi-Kyung</au><au>Li, Suiqiong</au><au>Chin, Bryan A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors</atitle><jtitle>Food and bioprocess technology</jtitle><stitle>Food Bioprocess Technol</stitle><date>2013-03-01</date><risdate>2013</risdate><volume>6</volume><issue>3</issue><spage>682</spage><epage>689</epage><pages>682-689</pages><issn>1935-5130</issn><eissn>1935-5149</eissn><abstract>A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables.
Salmonella typhimurium
was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions.
S. typhimurium
was inoculated on the surface of fresh tomatoes, and ME biosensors were used to detect the bacteria. The populations of
S. typhimurium
after 24-h incubation time at 37°C and 100% relative humidity were 6.1 and 7.8 logCFU/cm
2
after starting with initial inoculations of 3.0 and 5.0 logCFU/cm
2
, respectively. After evident growth, measurement sensors with E2 phage immobilized on their surfaces and control sensors devoid of E2 phage were placed on the inoculated tomato surfaces and the resonant frequency shifts were measured. As the population of
S. typhimurium
increased, the resonant frequency shifts of the measurement sensors significantly increased (
P
< 0.01), exhibiting 6,680 ± 665 and 9,384 ± 457 Hz for the populations of 6.1 and 7.8 logCFU/cm
2
, respectively. SEM images confirmed that the measurement sensor resonant frequency shifts were due to
S. typhimurium
binding with E2 phage. This study demonstrated that the ME biosensor method could detect
S. typhimurium
grown directly on tomato surfaces with limited sample preparation procedures. Therefore, the ME biosensor could be applied as a cost and time effective, relatively simple, practically suitable on-site detection method for
S. typhimurium
in fresh produce.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s11947-011-0708-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-5130 |
| ispartof | Food and bioprocess technology, 2013-03, Vol.6 (3), p.682-689 |
| issn | 1935-5130 1935-5149 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1430858981 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Biosensors Biotechnology Chemistry Chemistry and Materials Science Chemistry/Food Science Environmental conditions Food Science Inoculation Lycopersicon esculentum Nutrients Original Paper Phages Populations Relative humidity Resonant frequencies Salmonella Sample preparation Sensors Tomatoes |
| title | Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T05%3A49%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20Salmonella%20typhimurium%20Grown%20Directly%20on%20Tomato%20Surface%20Using%20Phage-Based%20Magnetoelastic%20Biosensors&rft.jtitle=Food%20and%20bioprocess%20technology&rft.au=Park,%20Mi-Kyung&rft.date=2013-03-01&rft.volume=6&rft.issue=3&rft.spage=682&rft.epage=689&rft.pages=682-689&rft.issn=1935-5130&rft.eissn=1935-5149&rft_id=info:doi/10.1007/s11947-011-0708-2&rft_dat=%3Cproquest_cross%3E1430858981%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2410777394&rft_id=info:pmid/&rfr_iscdi=true |