Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti-inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase
Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whil...
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| Veröffentlicht in: | Phytotherapy research 2013-09, Vol.27 (9), p.1288-1292 |
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| creator | Khan, Haroon Saeed, Muhammad Gilani, Anwarul Hassan Mehmood, Malik Hassan Rehman, Najeeb-ur Muhammad, Naveed Abbas, Muzaffar Haq, Ikram-ul |
| description | Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K+ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca2+ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca2+ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC50: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/ptr.4860 |
| format | Article |
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The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K+ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca2+ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca2+ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC50: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.4860</identifier><identifier>PMID: 23109174</identifier><identifier>CODEN: PHYREH</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; anti-inflammatory ; Anti-Inflammatory Agents - pharmacology ; bronchodilator ; Bronchodilator Agents - pharmacology ; Ca2+ channel blockers ; Calcium Channel Blockers - pharmacology ; Carbachol - adverse effects ; Carrageenan - adverse effects ; Edema - drug therapy ; Female ; In Vitro Techniques ; Inflammation - drug therapy ; Lipoxygenase ; Lipoxygenase Inhibitors - pharmacology ; Male ; Plant Components, Aerial - chemistry ; Plant Extracts - pharmacology ; Polygonatum - chemistry ; Polygonatum verticillatum ; Rabbits ; Rats ; Rats, Wistar ; Trachea - drug effects ; Verapamil - pharmacology</subject><ispartof>Phytotherapy research, 2013-09, Vol.27 (9), p.1288-1292</ispartof><rights>Copyright © 2012 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2013 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.4860$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.4860$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23109174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Haroon</creatorcontrib><creatorcontrib>Saeed, Muhammad</creatorcontrib><creatorcontrib>Gilani, Anwarul Hassan</creatorcontrib><creatorcontrib>Mehmood, Malik Hassan</creatorcontrib><creatorcontrib>Rehman, Najeeb-ur</creatorcontrib><creatorcontrib>Muhammad, Naveed</creatorcontrib><creatorcontrib>Abbas, Muzaffar</creatorcontrib><creatorcontrib>Haq, Ikram-ul</creatorcontrib><title>Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti-inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase</title><title>Phytotherapy research</title><addtitle>Phytother. Res</addtitle><description>Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K+ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca2+ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca2+ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC50: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>anti-inflammatory</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>bronchodilator</subject><subject>Bronchodilator Agents - pharmacology</subject><subject>Ca2+ channel blockers</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Carbachol - adverse effects</subject><subject>Carrageenan - adverse effects</subject><subject>Edema - drug therapy</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Inflammation - drug therapy</subject><subject>Lipoxygenase</subject><subject>Lipoxygenase Inhibitors - pharmacology</subject><subject>Male</subject><subject>Plant Components, Aerial - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Polygonatum - chemistry</subject><subject>Polygonatum verticillatum</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Trachea - drug effects</subject><subject>Verapamil - pharmacology</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c1u1DAQB3ALgehSkHgCZIkLEkprx3bicEtX0BatygqK2JvlJJOtS2IvtlOaZ-Flm7RlkTiNxv7p749B6DUlR5SQ9HgX_RGXGXmCFpQURUJFzp6iBSkETTiVmwP0IoRrQkiREv4cHaRsYjTnC_TnxDtbX7nGdDo6j8s6mhsTR-xaXII3usNr7WOY-7Xrxq2zOg49vgEfTW267r4rh20PNkKDqxGXNprE2LbTfT9njvvQD7iMEeygo3F2Tlzq9D1eXmlroQtY2wavzM7djluwOsBL9KzVXYBXj_UQff_08XJ5lqy-nJ4vy1ViUi5IAlyIihMuq7Zuapm1laS80BkUtRQsLwhhQvC8qaqKSp41rc6kntd0AS1njB2idw-5O-9-DRCi6k2oYXqbBTcERTkjUqSS84m-_Y9eu8Hb6Xb3imVsOnFSbx7VUPXQqJ03vfaj-vvtE0gewG_Twbjfp0TN41TTONU8TrW-_DrXf96ECLd7r_1PleUsF-rHxak625DNt89SqjW7A6W9osY</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Khan, Haroon</creator><creator>Saeed, Muhammad</creator><creator>Gilani, Anwarul Hassan</creator><creator>Mehmood, Malik Hassan</creator><creator>Rehman, Najeeb-ur</creator><creator>Muhammad, Naveed</creator><creator>Abbas, Muzaffar</creator><creator>Haq, Ikram-ul</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201309</creationdate><title>Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti-inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase</title><author>Khan, Haroon ; Saeed, Muhammad ; Gilani, Anwarul Hassan ; Mehmood, Malik Hassan ; Rehman, Najeeb-ur ; Muhammad, Naveed ; Abbas, Muzaffar ; Haq, Ikram-ul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2450-e455b4048bfcdc86fb8149a6e9c853790035547dbbb1846dfa68a0355a9ef4333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>anti-inflammatory</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>bronchodilator</topic><topic>Bronchodilator Agents - pharmacology</topic><topic>Ca2+ channel blockers</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Carbachol - adverse effects</topic><topic>Carrageenan - adverse effects</topic><topic>Edema - drug therapy</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Inflammation - drug therapy</topic><topic>Lipoxygenase</topic><topic>Lipoxygenase Inhibitors - pharmacology</topic><topic>Male</topic><topic>Plant Components, Aerial - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Polygonatum - chemistry</topic><topic>Polygonatum verticillatum</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Trachea - drug effects</topic><topic>Verapamil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Haroon</creatorcontrib><creatorcontrib>Saeed, Muhammad</creatorcontrib><creatorcontrib>Gilani, Anwarul Hassan</creatorcontrib><creatorcontrib>Mehmood, Malik Hassan</creatorcontrib><creatorcontrib>Rehman, Najeeb-ur</creatorcontrib><creatorcontrib>Muhammad, Naveed</creatorcontrib><creatorcontrib>Abbas, Muzaffar</creatorcontrib><creatorcontrib>Haq, Ikram-ul</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Haroon</au><au>Saeed, Muhammad</au><au>Gilani, Anwarul Hassan</au><au>Mehmood, Malik Hassan</au><au>Rehman, Najeeb-ur</au><au>Muhammad, Naveed</au><au>Abbas, Muzaffar</au><au>Haq, Ikram-ul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti-inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother. Res</addtitle><date>2013-09</date><risdate>2013</risdate><volume>27</volume><issue>9</issue><spage>1288</spage><epage>1292</epage><pages>1288-1292</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><coden>PHYREH</coden><abstract>Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan‐induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti‐inflammatory profile of PA. When tested against carbachol and K+ (80 mM)‐induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose‐dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca2+ concentration–response curves to the right, analogous to that produced by verapamil, confirming a Ca2+ channel blocker‐like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose‐dependent manner which was augmented by its prominent LOX inhibitory activity (IC50: 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions. Copyright © 2012 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23109174</pmid><doi>10.1002/ptr.4860</doi><tpages>5</tpages></addata></record> |
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| ispartof | Phytotherapy research, 2013-09, Vol.27 (9), p.1288-1292 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals anti-inflammatory Anti-Inflammatory Agents - pharmacology bronchodilator Bronchodilator Agents - pharmacology Ca2+ channel blockers Calcium Channel Blockers - pharmacology Carbachol - adverse effects Carrageenan - adverse effects Edema - drug therapy Female In Vitro Techniques Inflammation - drug therapy Lipoxygenase Lipoxygenase Inhibitors - pharmacology Male Plant Components, Aerial - chemistry Plant Extracts - pharmacology Polygonatum - chemistry Polygonatum verticillatum Rabbits Rats Rats, Wistar Trachea - drug effects Verapamil - pharmacology |
| title | Bronchodilator Activity of Aerial Parts of Polygonatum verticillatum Augmented by Anti-inflammatory Activity: Attenuation of Ca2+ Channels and Lipoxygenase |
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