The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival
Background Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymo...
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| Veröffentlicht in: | Cellular oncology (Dordrecht) 2011-08, Vol.34 (4), p.381-391 |
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| creator | Antonacopoulou, Anna G. Floratou, Konstantina Bravou, Vasiliki Kottorou, Anastasia Dimitrakopoulos, Fotinos-Ioannis Marousi, Stella Stavropoulos, Michalis Koutras, Angelos K. Scopa, Chrisoula D. Kalofonos, Haralabos P. |
| description | Background
Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386 C/T and -31 G/C polymorphisms were investigated.
Methods
Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR
Results
Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the -31 G/C snp may be related to CRC development and improved overall survival.
Conclusion
Our results support a role of survivin in colorectal carcinogenesis while the -31 G/C snp may constitute a marker of survival. |
| doi_str_mv | 10.1007/s13402-011-0038-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1430852522</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1430852522</sourcerecordid><originalsourceid>FETCH-LOGICAL-c376t-499ebabc6c1fb96eaffb34063d5bdb568587d9b9ba4c4127a0968ca505305ed43</originalsourceid><addsrcrecordid>eNp9kE9v1DAQxS0EotXSD8AF-cgl4P-bHKuqtEiVeilna-xMWFeOk9rJUm58dLzapdzqgz2233ua-RHykbMvnLHt18KlYqJhnDeMybZRb8i5EPUmlTRvX2rRnpGLUh5ZXcpwo817cia4li0T6pz8edghLWveh31ItJGcljTTWu7WERL1U5wy-gUi9ZA85vqSM0ZYsNBfYdn995Y5Bo90DzlAWig-zxlLCVOikHoaxjlPe-xp3TLEePJB_EDeDRALXpzODfnx7frh6ra5u7_5fnV513i5NUujug4dOG88H1xnEIbB1fmN7LXrnTatbrd95zoHyisutsA603rQTEumsVdyQz4fc2sfTyuWxY6heIwREk5rsVxJ1mqhhahSfpT6PJWScbBzDiPk35Yze2Bvj-xtZW8P7O0h_tMpfnUj9i-Of6SrQBwFpX6ln5jt47TmVEd-JfUvwweRXw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1430852522</pqid></control><display><type>article</type><title>The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Antonacopoulou, Anna G. ; Floratou, Konstantina ; Bravou, Vasiliki ; Kottorou, Anastasia ; Dimitrakopoulos, Fotinos-Ioannis ; Marousi, Stella ; Stavropoulos, Michalis ; Koutras, Angelos K. ; Scopa, Chrisoula D. ; Kalofonos, Haralabos P.</creator><creatorcontrib>Antonacopoulou, Anna G. ; Floratou, Konstantina ; Bravou, Vasiliki ; Kottorou, Anastasia ; Dimitrakopoulos, Fotinos-Ioannis ; Marousi, Stella ; Stavropoulos, Michalis ; Koutras, Angelos K. ; Scopa, Chrisoula D. ; Kalofonos, Haralabos P.</creatorcontrib><description>Background
Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386 C/T and -31 G/C polymorphisms were investigated.
Methods
Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR
Results
Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the -31 G/C snp may be related to CRC development and improved overall survival.
Conclusion
Our results support a role of survivin in colorectal carcinogenesis while the -31 G/C snp may constitute a marker of survival.</description><identifier>ISSN: 2211-3428</identifier><identifier>EISSN: 2211-3436</identifier><identifier>DOI: 10.1007/s13402-011-0038-4</identifier><identifier>PMID: 21538024</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aged ; Aged, 80 and over ; Alleles ; Alternative Splicing - genetics ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Cell Differentiation ; Colorectal Neoplasms - genetics ; Disease Progression ; Exons - genetics ; Female ; Gene Expression Regulation, Neoplastic ; Genetic Predisposition to Disease ; Humans ; Immunohistochemistry ; Inhibitor of Apoptosis Proteins - genetics ; Male ; Middle Aged ; Oncology ; Original Paper ; Pathology ; Polymorphism, Single Nucleotide - genetics ; Protein Transport ; Survival Analysis</subject><ispartof>Cellular oncology (Dordrecht), 2011-08, Vol.34 (4), p.381-391</ispartof><rights>International Society for Cellular Oncology 2011</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-499ebabc6c1fb96eaffb34063d5bdb568587d9b9ba4c4127a0968ca505305ed43</citedby><cites>FETCH-LOGICAL-c376t-499ebabc6c1fb96eaffb34063d5bdb568587d9b9ba4c4127a0968ca505305ed43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13402-011-0038-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13402-011-0038-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21538024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Antonacopoulou, Anna G.</creatorcontrib><creatorcontrib>Floratou, Konstantina</creatorcontrib><creatorcontrib>Bravou, Vasiliki</creatorcontrib><creatorcontrib>Kottorou, Anastasia</creatorcontrib><creatorcontrib>Dimitrakopoulos, Fotinos-Ioannis</creatorcontrib><creatorcontrib>Marousi, Stella</creatorcontrib><creatorcontrib>Stavropoulos, Michalis</creatorcontrib><creatorcontrib>Koutras, Angelos K.</creatorcontrib><creatorcontrib>Scopa, Chrisoula D.</creatorcontrib><creatorcontrib>Kalofonos, Haralabos P.</creatorcontrib><title>The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival</title><title>Cellular oncology (Dordrecht)</title><addtitle>Cell Oncol</addtitle><addtitle>Cell Oncol (Dordr)</addtitle><description>Background
Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386 C/T and -31 G/C polymorphisms were investigated.
Methods
Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR
Results
Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the -31 G/C snp may be related to CRC development and improved overall survival.
Conclusion
Our results support a role of survivin in colorectal carcinogenesis while the -31 G/C snp may constitute a marker of survival.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alleles</subject><subject>Alternative Splicing - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cell Differentiation</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Disease Progression</subject><subject>Exons - genetics</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic Predisposition to Disease</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Inhibitor of Apoptosis Proteins - genetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Original Paper</subject><subject>Pathology</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Protein Transport</subject><subject>Survival Analysis</subject><issn>2211-3428</issn><issn>2211-3436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9v1DAQxS0EotXSD8AF-cgl4P-bHKuqtEiVeilna-xMWFeOk9rJUm58dLzapdzqgz2233ua-RHykbMvnLHt18KlYqJhnDeMybZRb8i5EPUmlTRvX2rRnpGLUh5ZXcpwo817cia4li0T6pz8edghLWveh31ItJGcljTTWu7WERL1U5wy-gUi9ZA85vqSM0ZYsNBfYdn995Y5Bo90DzlAWig-zxlLCVOikHoaxjlPe-xp3TLEePJB_EDeDRALXpzODfnx7frh6ra5u7_5fnV513i5NUujug4dOG88H1xnEIbB1fmN7LXrnTatbrd95zoHyisutsA603rQTEumsVdyQz4fc2sfTyuWxY6heIwREk5rsVxJ1mqhhahSfpT6PJWScbBzDiPk35Yze2Bvj-xtZW8P7O0h_tMpfnUj9i-Of6SrQBwFpX6ln5jt47TmVEd-JfUvwweRXw</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Antonacopoulou, Anna G.</creator><creator>Floratou, Konstantina</creator><creator>Bravou, Vasiliki</creator><creator>Kottorou, Anastasia</creator><creator>Dimitrakopoulos, Fotinos-Ioannis</creator><creator>Marousi, Stella</creator><creator>Stavropoulos, Michalis</creator><creator>Koutras, Angelos K.</creator><creator>Scopa, Chrisoula D.</creator><creator>Kalofonos, Haralabos P.</creator><general>Springer Netherlands</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>20110801</creationdate><title>The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival</title><author>Antonacopoulou, Anna G. ; Floratou, Konstantina ; Bravou, Vasiliki ; Kottorou, Anastasia ; Dimitrakopoulos, Fotinos-Ioannis ; Marousi, Stella ; Stavropoulos, Michalis ; Koutras, Angelos K. ; Scopa, Chrisoula D. ; Kalofonos, Haralabos P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-499ebabc6c1fb96eaffb34063d5bdb568587d9b9ba4c4127a0968ca505305ed43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alleles</topic><topic>Alternative Splicing - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cell Differentiation</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Disease Progression</topic><topic>Exons - genetics</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic Predisposition to Disease</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Inhibitor of Apoptosis Proteins - genetics</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Original Paper</topic><topic>Pathology</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Protein Transport</topic><topic>Survival Analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Antonacopoulou, Anna G.</creatorcontrib><creatorcontrib>Floratou, Konstantina</creatorcontrib><creatorcontrib>Bravou, Vasiliki</creatorcontrib><creatorcontrib>Kottorou, Anastasia</creatorcontrib><creatorcontrib>Dimitrakopoulos, Fotinos-Ioannis</creatorcontrib><creatorcontrib>Marousi, Stella</creatorcontrib><creatorcontrib>Stavropoulos, Michalis</creatorcontrib><creatorcontrib>Koutras, Angelos K.</creatorcontrib><creatorcontrib>Scopa, Chrisoula D.</creatorcontrib><creatorcontrib>Kalofonos, Haralabos P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Cellular oncology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Antonacopoulou, Anna G.</au><au>Floratou, Konstantina</au><au>Bravou, Vasiliki</au><au>Kottorou, Anastasia</au><au>Dimitrakopoulos, Fotinos-Ioannis</au><au>Marousi, Stella</au><au>Stavropoulos, Michalis</au><au>Koutras, Angelos K.</au><au>Scopa, Chrisoula D.</au><au>Kalofonos, Haralabos P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival</atitle><jtitle>Cellular oncology (Dordrecht)</jtitle><stitle>Cell Oncol</stitle><addtitle>Cell Oncol (Dordr)</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>34</volume><issue>4</issue><spage>381</spage><epage>391</epage><pages>381-391</pages><issn>2211-3428</issn><eissn>2211-3436</eissn><abstract>Background
Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386 C/T and -31 G/C polymorphisms were investigated.
Methods
Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR
Results
Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the -31 G/C snp may be related to CRC development and improved overall survival.
Conclusion
Our results support a role of survivin in colorectal carcinogenesis while the -31 G/C snp may constitute a marker of survival.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21538024</pmid><doi>10.1007/s13402-011-0038-4</doi><tpages>11</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Alleles Alternative Splicing - genetics Biomedical and Life Sciences Biomedicine Cancer Research Cell Differentiation Colorectal Neoplasms - genetics Disease Progression Exons - genetics Female Gene Expression Regulation, Neoplastic Genetic Predisposition to Disease Humans Immunohistochemistry Inhibitor of Apoptosis Proteins - genetics Male Middle Aged Oncology Original Paper Pathology Polymorphism, Single Nucleotide - genetics Protein Transport Survival Analysis |
| title | The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival |
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