An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation
Contents The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PB...
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| Veröffentlicht in: | Reproduction in domestic animals 2013-08, Vol.48 (4), p.554-561 |
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| creator | Woodward, EM Christoffersen, M Campos, J Horohov, DW Scoggin, KE Squires, E Troedsson, MHT |
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The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post‐breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047). |
| doi_str_mv | 10.1111/rda.12124 |
| format | Article |
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The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post‐breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047).</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12124</identifier><identifier>PMID: 23228000</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animal diseases ; Animal reproduction ; Animals ; Biopsy ; Breeding ; Cell Wall - immunology ; Dexamethasone - administration & dosage ; Disease Resistance ; Disease Susceptibility - immunology ; Disease Susceptibility - veterinary ; Endometritis - etiology ; Endometritis - immunology ; Endometritis - veterinary ; Endometrium - enzymology ; Female ; Hormones ; Horse Diseases - etiology ; Horse Diseases - immunology ; Horses ; Horses - immunology ; Horses - metabolism ; Immunomodulation ; Insemination, Artificial - veterinary ; Mycobacterium ; Mycobacterium - immunology ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase Type II - genetics ; Nitric Oxide Synthase Type II - metabolism ; Polymerase Chain Reaction - veterinary ; RNA, Messenger - analysis ; Uterus - metabolism</subject><ispartof>Reproduction in domestic animals, 2013-08, Vol.48 (4), p.554-561</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>2012 Blackwell Verlag GmbH.</rights><rights>Copyright © 2013 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4594-436e1ad26947f873ec10bcd75a960fe1659dde1d2954f67e82982ae3ebd6d8503</citedby><cites>FETCH-LOGICAL-c4594-436e1ad26947f873ec10bcd75a960fe1659dde1d2954f67e82982ae3ebd6d8503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12124$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12124$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23228000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woodward, EM</creatorcontrib><creatorcontrib>Christoffersen, M</creatorcontrib><creatorcontrib>Campos, J</creatorcontrib><creatorcontrib>Horohov, DW</creatorcontrib><creatorcontrib>Scoggin, KE</creatorcontrib><creatorcontrib>Squires, E</creatorcontrib><creatorcontrib>Troedsson, MHT</creatorcontrib><title>An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post‐breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047).</description><subject>Animal diseases</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biopsy</subject><subject>Breeding</subject><subject>Cell Wall - immunology</subject><subject>Dexamethasone - administration & dosage</subject><subject>Disease Resistance</subject><subject>Disease Susceptibility - immunology</subject><subject>Disease Susceptibility - veterinary</subject><subject>Endometritis - etiology</subject><subject>Endometritis - immunology</subject><subject>Endometritis - veterinary</subject><subject>Endometrium - enzymology</subject><subject>Female</subject><subject>Hormones</subject><subject>Horse Diseases - etiology</subject><subject>Horse Diseases - immunology</subject><subject>Horses</subject><subject>Horses - immunology</subject><subject>Horses - metabolism</subject><subject>Immunomodulation</subject><subject>Insemination, Artificial - veterinary</subject><subject>Mycobacterium</subject><subject>Mycobacterium - immunology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase Type II - genetics</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>RNA, Messenger - analysis</subject><subject>Uterus - metabolism</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokNhwQsgS2xgkdY_sZMsp53pMGL6o0LF0vLEN8UlcQbbKe0L8Zw4M20XSEh4c3Wl7xzfew9Cbyk5oOkdeqMPKKMsf4YmNOdVRgSnz9GEVFxmspDlHnoVwg0hVJRF8RLtMc5YSQiZoN9Th5fuFkK01zra3uG-wVcRvHWAz2z0tsbnd9YAvvC9GeotYh0-1R4C_jKEGjbRrlvA2hl8CcGGqF3EsccX4McOUnfkAYx119nSJQ8weO5M30FyjzZslfE74HnTQB3DOMGy6wbXd-nHdjvVa_Si0W2ANw91H12dzL8ef8pW54vl8XSV1bmo8iznEqg2TFZ50ZQFh5qSdW0KoStJGqBSVMYANawSeSMLKFlVMg0c1kaaUhC-jz7sfDe-_zmkq6jOphXbVjvoh6DSdUniJKX_gRLCq4qJIqHv_0Jv-sG7tIiiCRGccD5SH3dU7fsQPDRq422n_b2iRI05q5Sz2uac2HcPjsO6A_NEPgabgMMd8Mu2cP9vJ3U5mz5aZjvFmNndk0L7H0oWvBDq29lCrWYLcko-r9SM_wHw8sJE</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Woodward, EM</creator><creator>Christoffersen, M</creator><creator>Campos, J</creator><creator>Horohov, DW</creator><creator>Scoggin, KE</creator><creator>Squires, E</creator><creator>Troedsson, MHT</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201308</creationdate><title>An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation</title><author>Woodward, EM ; Christoffersen, M ; Campos, J ; Horohov, DW ; Scoggin, KE ; Squires, E ; Troedsson, MHT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4594-436e1ad26947f873ec10bcd75a960fe1659dde1d2954f67e82982ae3ebd6d8503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal diseases</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biopsy</topic><topic>Breeding</topic><topic>Cell Wall - immunology</topic><topic>Dexamethasone - administration & dosage</topic><topic>Disease Resistance</topic><topic>Disease Susceptibility - immunology</topic><topic>Disease Susceptibility - veterinary</topic><topic>Endometritis - etiology</topic><topic>Endometritis - immunology</topic><topic>Endometritis - veterinary</topic><topic>Endometrium - enzymology</topic><topic>Female</topic><topic>Hormones</topic><topic>Horse Diseases - etiology</topic><topic>Horse Diseases - immunology</topic><topic>Horses</topic><topic>Horses - immunology</topic><topic>Horses - metabolism</topic><topic>Immunomodulation</topic><topic>Insemination, Artificial - veterinary</topic><topic>Mycobacterium</topic><topic>Mycobacterium - immunology</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase Type II - genetics</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>RNA, Messenger - analysis</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woodward, EM</creatorcontrib><creatorcontrib>Christoffersen, M</creatorcontrib><creatorcontrib>Campos, J</creatorcontrib><creatorcontrib>Horohov, DW</creatorcontrib><creatorcontrib>Scoggin, KE</creatorcontrib><creatorcontrib>Squires, E</creatorcontrib><creatorcontrib>Troedsson, MHT</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woodward, EM</au><au>Christoffersen, M</au><au>Campos, J</au><au>Horohov, DW</au><au>Scoggin, KE</au><au>Squires, E</au><au>Troedsson, MHT</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2013-08</date><risdate>2013</risdate><volume>48</volume><issue>4</issue><spage>554</spage><epage>561</epage><pages>554-561</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post‐breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047).</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>23228000</pmid><doi>10.1111/rda.12124</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproduction in domestic animals, 2013-08, Vol.48 (4), p.554-561 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal diseases Animal reproduction Animals Biopsy Breeding Cell Wall - immunology Dexamethasone - administration & dosage Disease Resistance Disease Susceptibility - immunology Disease Susceptibility - veterinary Endometritis - etiology Endometritis - immunology Endometritis - veterinary Endometrium - enzymology Female Hormones Horse Diseases - etiology Horse Diseases - immunology Horses Horses - immunology Horses - metabolism Immunomodulation Insemination, Artificial - veterinary Mycobacterium Mycobacterium - immunology Nitric oxide Nitric Oxide - biosynthesis Nitric Oxide Synthase Type II - genetics Nitric Oxide Synthase Type II - metabolism Polymerase Chain Reaction - veterinary RNA, Messenger - analysis Uterus - metabolism |
| title | An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding-Induced Endometritis and the Effects of Immunomodulation |
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