High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets
A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4...
Gespeichert in:
| Veröffentlicht in: | Journal of AOAC International 2013-07, Vol.96 (4), p.730-736 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 736 |
|---|---|
| container_issue | 4 |
| container_start_page | 730 |
| container_title | Journal of AOAC International |
| container_volume | 96 |
| creator | Gindy, Alaa El Nassar, Mohammad Wafaa Attia, Khalid Abdel-Salam Abu-Seada, Hamed Hamed Ghandour, Mahmoud El |
| description | A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines. |
| doi_str_mv | 10.5740/jaoacint.11-073 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1430398495</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A370395862</galeid><sourcerecordid>A370395862</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-771a9e21e2b79f6af0f5cf5bd0cc2c1534b2be98920ce9508e14a9c134e2d2753</originalsourceid><addsrcrecordid>eNpt0Utr3DAUBWBTGppHu-6uGEohG0_0smUtwzRtAgPJIll0Ja7lqxkFW5pImkX-fTXMpKVQtJAQ37lcOFX1mZJFKwW5eoYAxvm8oLQhkr-rzqgSopGKsfflTTrScCbpaXWe0jMhgnaEfahOmSCESCHOql-3br1pHjDaEGfwBuuVe9m5sV5uYpghh3WE7caZ-jtmjLPzkF3wdbD1Td7AnF4nyFg7Xz9F57EGP9aPMEyY08fqxMKU8NPxvqieftw8Lm-b1f3Pu-X1qjGCt7mRkoJCRpENUtkOLLGtse0wEmOYoS0XAxtQ9YoRg6olPVIBylAukI1MtvyiujzM3cbwssOU9eySwWkCj2GXNBWccNULtadfD3QNE2rnbcgRzJ7ray6LavuOFbX4jypnxNmZ4NG68v9P4OoQMDGkFNHqbXQzxFdNid7XpN9q0pTqUlNJfDnuvBtmHP_4t14K-HYEkAxMNpZqXPrrZMd70XX8Nwuzm4I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1430398495</pqid></control><display><type>article</type><title>High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Gindy, Alaa El ; Nassar, Mohammad Wafaa ; Attia, Khalid Abdel-Salam ; Abu-Seada, Hamed Hamed ; Ghandour, Mahmoud El</creator><creatorcontrib>Gindy, Alaa El ; Nassar, Mohammad Wafaa ; Attia, Khalid Abdel-Salam ; Abu-Seada, Hamed Hamed ; Ghandour, Mahmoud El</creatorcontrib><description>A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.5740/jaoacint.11-073</identifier><identifier>PMID: 24000744</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>Adult ; Analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Ethamsylate - analysis ; Ethamsylate - urine ; Fundamental and applied biological sciences. Psychology ; Hemostatics - analysis ; High performance liquid chromatography ; Humans ; Hydrogen-Ion Concentration ; Limit of Detection ; Male ; Methods ; Reproducibility of Results ; Tablets - chemistry ; Urine</subject><ispartof>Journal of AOAC International, 2013-07, Vol.96 (4), p.730-736</ispartof><rights>2014 INIST-CNRS</rights><rights>COPYRIGHT 2013 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-771a9e21e2b79f6af0f5cf5bd0cc2c1534b2be98920ce9508e14a9c134e2d2753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27638466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24000744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gindy, Alaa El</creatorcontrib><creatorcontrib>Nassar, Mohammad Wafaa</creatorcontrib><creatorcontrib>Attia, Khalid Abdel-Salam</creatorcontrib><creatorcontrib>Abu-Seada, Hamed Hamed</creatorcontrib><creatorcontrib>Ghandour, Mahmoud El</creatorcontrib><title>High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines.</description><subject>Adult</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Ethamsylate - analysis</subject><subject>Ethamsylate - urine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemostatics - analysis</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Limit of Detection</subject><subject>Male</subject><subject>Methods</subject><subject>Reproducibility of Results</subject><subject>Tablets - chemistry</subject><subject>Urine</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0Utr3DAUBWBTGppHu-6uGEohG0_0smUtwzRtAgPJIll0Ja7lqxkFW5pImkX-fTXMpKVQtJAQ37lcOFX1mZJFKwW5eoYAxvm8oLQhkr-rzqgSopGKsfflTTrScCbpaXWe0jMhgnaEfahOmSCESCHOql-3br1pHjDaEGfwBuuVe9m5sV5uYpghh3WE7caZ-jtmjLPzkF3wdbD1Td7AnF4nyFg7Xz9F57EGP9aPMEyY08fqxMKU8NPxvqieftw8Lm-b1f3Pu-X1qjGCt7mRkoJCRpENUtkOLLGtse0wEmOYoS0XAxtQ9YoRg6olPVIBylAukI1MtvyiujzM3cbwssOU9eySwWkCj2GXNBWccNULtadfD3QNE2rnbcgRzJ7ray6LavuOFbX4jypnxNmZ4NG68v9P4OoQMDGkFNHqbXQzxFdNid7XpN9q0pTqUlNJfDnuvBtmHP_4t14K-HYEkAxMNpZqXPrrZMd70XX8Nwuzm4I</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Gindy, Alaa El</creator><creator>Nassar, Mohammad Wafaa</creator><creator>Attia, Khalid Abdel-Salam</creator><creator>Abu-Seada, Hamed Hamed</creator><creator>Ghandour, Mahmoud El</creator><general>AOAC International</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets</title><author>Gindy, Alaa El ; Nassar, Mohammad Wafaa ; Attia, Khalid Abdel-Salam ; Abu-Seada, Hamed Hamed ; Ghandour, Mahmoud El</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-771a9e21e2b79f6af0f5cf5bd0cc2c1534b2be98920ce9508e14a9c134e2d2753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Ethamsylate - analysis</topic><topic>Ethamsylate - urine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemostatics - analysis</topic><topic>High performance liquid chromatography</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Limit of Detection</topic><topic>Male</topic><topic>Methods</topic><topic>Reproducibility of Results</topic><topic>Tablets - chemistry</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gindy, Alaa El</creatorcontrib><creatorcontrib>Nassar, Mohammad Wafaa</creatorcontrib><creatorcontrib>Attia, Khalid Abdel-Salam</creatorcontrib><creatorcontrib>Abu-Seada, Hamed Hamed</creatorcontrib><creatorcontrib>Ghandour, Mahmoud El</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of AOAC International</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gindy, Alaa El</au><au>Nassar, Mohammad Wafaa</au><au>Attia, Khalid Abdel-Salam</au><au>Abu-Seada, Hamed Hamed</au><au>Ghandour, Mahmoud El</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets</atitle><jtitle>Journal of AOAC International</jtitle><addtitle>J AOAC Int</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>96</volume><issue>4</issue><spage>730</spage><epage>736</epage><pages>730-736</pages><issn>1060-3271</issn><eissn>1944-7922</eissn><abstract>A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>24000744</pmid><doi>10.5740/jaoacint.11-073</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1060-3271 |
| ispartof | Journal of AOAC International, 2013-07, Vol.96 (4), p.730-736 |
| issn | 1060-3271 1944-7922 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1430398495 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Analysis Biological and medical sciences Chromatography, High Pressure Liquid - methods Ethamsylate - analysis Ethamsylate - urine Fundamental and applied biological sciences. Psychology Hemostatics - analysis High performance liquid chromatography Humans Hydrogen-Ion Concentration Limit of Detection Male Methods Reproducibility of Results Tablets - chemistry Urine |
| title | High-Performance Liquid Chromatographic Determination of Ethamsylate in Urine and Tablets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T23%3A29%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-Performance%20Liquid%20Chromatographic%20Determination%20of%20Ethamsylate%20in%20Urine%20and%20Tablets&rft.jtitle=Journal%20of%20AOAC%20International&rft.au=Gindy,%20Alaa%20El&rft.date=2013-07-01&rft.volume=96&rft.issue=4&rft.spage=730&rft.epage=736&rft.pages=730-736&rft.issn=1060-3271&rft.eissn=1944-7922&rft_id=info:doi/10.5740/jaoacint.11-073&rft_dat=%3Cgale_proqu%3EA370395862%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1430398495&rft_id=info:pmid/24000744&rft_galeid=A370395862&rfr_iscdi=true |