Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots
The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photoluminescence properties and stability of water-...
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| Veröffentlicht in: | Chinese science bulletin 2013-07, Vol.58 (21), p.2616-2621 |
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| creator | Zhang, YouLin Tu, LangPing Zeng, QingHui Kong, XiangGui |
| description | The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields. |
| doi_str_mv | 10.1007/s11434-013-5716-3 |
| format | Article |
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In this paper, the effects of immunoglobulin G (IgG) on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.</description><identifier>ISSN: 1001-6538</identifier><identifier>EISSN: 1861-9541</identifier><identifier>DOI: 10.1007/s11434-013-5716-3</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>acrylic acid ; avidin ; bovine serum albumin ; Cadmium selenides ; CdSe ; Chemistry/Food Science ; Coating ; coatings ; Earth Sciences ; Engineering ; goats ; Humanities and Social Sciences ; humans ; immunoglobulin G ; Intermetallics ; Life Sciences ; multidisciplinary ; optical properties ; Photoluminescence ; Physics ; protein structure ; Proteins ; Quantum dots ; rabbits ; Science ; Science (multidisciplinary) ; Stability ; Zinc sulfides ; ZnS ; 光致发光特性 ; 抗生物素蛋白 ; 水溶性 ; 稳定性 ; 蛋白质分子 ; 量子点</subject><ispartof>Chinese science bulletin, 2013-07, Vol.58 (21), p.2616-2621</ispartof><rights>The Author(s) 2013</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-d9095f9e282ff33b43f60c119e44e3dcd35a3ae77cd9f8a95063529ab9d1a7c53</citedby><cites>FETCH-LOGICAL-c480t-d9095f9e282ff33b43f60c119e44e3dcd35a3ae77cd9f8a95063529ab9d1a7c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86894X/86894X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Zhang, YouLin</creatorcontrib><creatorcontrib>Tu, LangPing</creatorcontrib><creatorcontrib>Zeng, QingHui</creatorcontrib><creatorcontrib>Kong, XiangGui</creatorcontrib><title>Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots</title><title>Chinese science bulletin</title><addtitle>Chin. Sci. Bull</addtitle><addtitle>Chinese Science Bulletin</addtitle><description>The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.</description><subject>acrylic acid</subject><subject>avidin</subject><subject>bovine serum albumin</subject><subject>Cadmium selenides</subject><subject>CdSe</subject><subject>Chemistry/Food Science</subject><subject>Coating</subject><subject>coatings</subject><subject>Earth Sciences</subject><subject>Engineering</subject><subject>goats</subject><subject>Humanities and Social Sciences</subject><subject>humans</subject><subject>immunoglobulin G</subject><subject>Intermetallics</subject><subject>Life Sciences</subject><subject>multidisciplinary</subject><subject>optical properties</subject><subject>Photoluminescence</subject><subject>Physics</subject><subject>protein structure</subject><subject>Proteins</subject><subject>Quantum dots</subject><subject>rabbits</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Stability</subject><subject>Zinc sulfides</subject><subject>ZnS</subject><subject>光致发光特性</subject><subject>抗生物素蛋白</subject><subject>水溶性</subject><subject>稳定性</subject><subject>蛋白质分子</subject><subject>量子点</subject><issn>1001-6538</issn><issn>1861-9541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kb1uFDEUhS0EEmHhAagwHY0T_86MS7RKIFIkiiUNjeX1XO9O5LF3bY9QKl4dryaipLq-0neOzj1G6COj14zS_qYwJoUklAmietYR8QpdsaFjRCvJXrc3pYx0Sgxv0btSntomWM-v0J9b78FVnDw-5VRhinhOAdwSoOAUcT0CPh1TTWGZpwjFQXRwQU-Q69QYG0dcqt1PYarPF5vftkImpQn2AfB23MHNr7jDLmUg5Qgh4PNiY11mPKZa3qM33oYCH17mBj3e3f7cficPP77db78-ECcHWsmoqVZeAx-490LspfAddYxpkBLE6EahrLDQ927UfrBa0U4oru1ej8z2TokN-rL6tujnBUo189SOCcFGSEsxTHI9DFJ0uqFsRV1OpWTw5pSn2eZnw6i5lG3Wsk3r0FzKNqJp-KopjY0HyOYpLTm2i_4r-rSKvE3GHvJUzOOOUybb73AhGrlBn1-iHFM8nJvzvyyyk1xpKsVfyJmYsg</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Zhang, YouLin</creator><creator>Tu, LangPing</creator><creator>Zeng, QingHui</creator><creator>Kong, XiangGui</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>FBQ</scope><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20130701</creationdate><title>Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots</title><author>Zhang, YouLin ; Tu, LangPing ; Zeng, QingHui ; Kong, XiangGui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-d9095f9e282ff33b43f60c119e44e3dcd35a3ae77cd9f8a95063529ab9d1a7c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>acrylic acid</topic><topic>avidin</topic><topic>bovine serum albumin</topic><topic>Cadmium selenides</topic><topic>CdSe</topic><topic>Chemistry/Food Science</topic><topic>Coating</topic><topic>coatings</topic><topic>Earth Sciences</topic><topic>Engineering</topic><topic>goats</topic><topic>Humanities and Social Sciences</topic><topic>humans</topic><topic>immunoglobulin G</topic><topic>Intermetallics</topic><topic>Life Sciences</topic><topic>multidisciplinary</topic><topic>optical properties</topic><topic>Photoluminescence</topic><topic>Physics</topic><topic>protein structure</topic><topic>Proteins</topic><topic>Quantum dots</topic><topic>rabbits</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Stability</topic><topic>Zinc sulfides</topic><topic>ZnS</topic><topic>光致发光特性</topic><topic>抗生物素蛋白</topic><topic>水溶性</topic><topic>稳定性</topic><topic>蛋白质分子</topic><topic>量子点</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, YouLin</creatorcontrib><creatorcontrib>Tu, LangPing</creatorcontrib><creatorcontrib>Zeng, QingHui</creatorcontrib><creatorcontrib>Kong, XiangGui</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Chinese science bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, YouLin</au><au>Tu, LangPing</au><au>Zeng, QingHui</au><au>Kong, XiangGui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots</atitle><jtitle>Chinese science bulletin</jtitle><stitle>Chin. Sci. Bull</stitle><addtitle>Chinese Science Bulletin</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>58</volume><issue>21</issue><spage>2616</spage><epage>2621</epage><pages>2616-2621</pages><issn>1001-6538</issn><eissn>1861-9541</eissn><abstract>The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s11434-013-5716-3</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acrylic acid avidin bovine serum albumin Cadmium selenides CdSe Chemistry/Food Science Coating coatings Earth Sciences Engineering goats Humanities and Social Sciences humans immunoglobulin G Intermetallics Life Sciences multidisciplinary optical properties Photoluminescence Physics protein structure Proteins Quantum dots rabbits Science Science (multidisciplinary) Stability Zinc sulfides ZnS 光致发光特性 抗生物素蛋白 水溶性 稳定性 蛋白质分子 量子点 |
| title | Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots |
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