Capture of viable circulating tumor cells in the liver of colorectal cancer patients
The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection. We detected CTCs in the peripheral and mesenteric blood of colo...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2013-09, Vol.59 (9), p.1384-1392 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1392 |
|---|---|
| container_issue | 9 |
| container_start_page | 1384 |
| container_title | Clinical chemistry (Baltimore, Md.) |
| container_volume | 59 |
| creator | Denève, Eric Riethdorf, Sabine Ramos, Jeanne Nocca, David Coffy, Amandine Daurès, Jean-Pierre Maudelonde, Thierry Fabre, Jean-Michel Pantel, Klaus Alix-Panabières, Catherine |
| description | The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.
We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.
The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).
A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients. |
| doi_str_mv | 10.1373/clinchem.2013.202846 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1429218905</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3109838781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-acaa92e63f3bb5335acc1fa2a521bc3449fa923e48e3d21b86d2a05a481738b53</originalsourceid><addsrcrecordid>eNpdkE1LAzEQhoMotlb_gUjAi5et-dzdHKX4BQUv9RyyadamZDc1yRb892Zp68FLhsw87zA8ANxiNMe0oo_a2V5vTDcnCNP8kJqVZ2CKOUVFzUt8DqYIIVEIzKoJuIpxm7-sqstLMCG0FJyIagpWC7VLQzDQt3BvVeMM1Dbowalk-y-Yhs4HqI1zEdoepo2Bzu5NGHHtnQ9GJ-WgVr3OzV0OmT7Fa3DRKhfNzbHOwOfL82rxViw_Xt8XT8tCM1alQmmlBDElbWnTcEq50hq3iihOcKMpY6LNc2pYbeg6t-pyTRTiitW4onVOzMDDYe8u-O_BxCQ7G8djVW_8ECVmRBBcCzSi9__QrR9Cn6_LFOOUC85Gih0oHXyMwbRyF2ynwo_ESI7W5cm6HK3Lg_UcuzsuH5rOrP9CJ830F2m6f7Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1445359545</pqid></control><display><type>article</type><title>Capture of viable circulating tumor cells in the liver of colorectal cancer patients</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Denève, Eric ; Riethdorf, Sabine ; Ramos, Jeanne ; Nocca, David ; Coffy, Amandine ; Daurès, Jean-Pierre ; Maudelonde, Thierry ; Fabre, Jean-Michel ; Pantel, Klaus ; Alix-Panabières, Catherine</creator><creatorcontrib>Denève, Eric ; Riethdorf, Sabine ; Ramos, Jeanne ; Nocca, David ; Coffy, Amandine ; Daurès, Jean-Pierre ; Maudelonde, Thierry ; Fabre, Jean-Michel ; Pantel, Klaus ; Alix-Panabières, Catherine</creatorcontrib><description>The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.
We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.
The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).
A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2013.202846</identifier><identifier>PMID: 23695297</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Agreements ; Biological properties ; Colon ; Colon - pathology ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - diagnosis ; Colorectal Neoplasms - pathology ; Female ; Humans ; Liver ; Liver - pathology ; Male ; Methods ; Middle Aged ; Neoplastic Cells, Circulating - pathology ; Prognosis ; Rectum - pathology ; Studies ; Surgery ; Tumors</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2013-09, Vol.59 (9), p.1384-1392</ispartof><rights>Copyright American Association for Clinical Chemistry Sep 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-acaa92e63f3bb5335acc1fa2a521bc3449fa923e48e3d21b86d2a05a481738b53</citedby><cites>FETCH-LOGICAL-c447t-acaa92e63f3bb5335acc1fa2a521bc3449fa923e48e3d21b86d2a05a481738b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23695297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Denève, Eric</creatorcontrib><creatorcontrib>Riethdorf, Sabine</creatorcontrib><creatorcontrib>Ramos, Jeanne</creatorcontrib><creatorcontrib>Nocca, David</creatorcontrib><creatorcontrib>Coffy, Amandine</creatorcontrib><creatorcontrib>Daurès, Jean-Pierre</creatorcontrib><creatorcontrib>Maudelonde, Thierry</creatorcontrib><creatorcontrib>Fabre, Jean-Michel</creatorcontrib><creatorcontrib>Pantel, Klaus</creatorcontrib><creatorcontrib>Alix-Panabières, Catherine</creatorcontrib><title>Capture of viable circulating tumor cells in the liver of colorectal cancer patients</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.
We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.
The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).
A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Agreements</subject><subject>Biological properties</subject><subject>Colon</subject><subject>Colon - pathology</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - blood</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Liver</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Prognosis</subject><subject>Rectum - pathology</subject><subject>Studies</subject><subject>Surgery</subject><subject>Tumors</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkE1LAzEQhoMotlb_gUjAi5et-dzdHKX4BQUv9RyyadamZDc1yRb892Zp68FLhsw87zA8ANxiNMe0oo_a2V5vTDcnCNP8kJqVZ2CKOUVFzUt8DqYIIVEIzKoJuIpxm7-sqstLMCG0FJyIagpWC7VLQzDQt3BvVeMM1Dbowalk-y-Yhs4HqI1zEdoepo2Bzu5NGHHtnQ9GJ-WgVr3OzV0OmT7Fa3DRKhfNzbHOwOfL82rxViw_Xt8XT8tCM1alQmmlBDElbWnTcEq50hq3iihOcKMpY6LNc2pYbeg6t-pyTRTiitW4onVOzMDDYe8u-O_BxCQ7G8djVW_8ECVmRBBcCzSi9__QrR9Cn6_LFOOUC85Gih0oHXyMwbRyF2ynwo_ESI7W5cm6HK3Lg_UcuzsuH5rOrP9CJ830F2m6f7Y</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Denève, Eric</creator><creator>Riethdorf, Sabine</creator><creator>Ramos, Jeanne</creator><creator>Nocca, David</creator><creator>Coffy, Amandine</creator><creator>Daurès, Jean-Pierre</creator><creator>Maudelonde, Thierry</creator><creator>Fabre, Jean-Michel</creator><creator>Pantel, Klaus</creator><creator>Alix-Panabières, Catherine</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>201309</creationdate><title>Capture of viable circulating tumor cells in the liver of colorectal cancer patients</title><author>Denève, Eric ; Riethdorf, Sabine ; Ramos, Jeanne ; Nocca, David ; Coffy, Amandine ; Daurès, Jean-Pierre ; Maudelonde, Thierry ; Fabre, Jean-Michel ; Pantel, Klaus ; Alix-Panabières, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-acaa92e63f3bb5335acc1fa2a521bc3449fa923e48e3d21b86d2a05a481738b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Agreements</topic><topic>Biological properties</topic><topic>Colon</topic><topic>Colon - pathology</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - blood</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Liver</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Prognosis</topic><topic>Rectum - pathology</topic><topic>Studies</topic><topic>Surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Denève, Eric</creatorcontrib><creatorcontrib>Riethdorf, Sabine</creatorcontrib><creatorcontrib>Ramos, Jeanne</creatorcontrib><creatorcontrib>Nocca, David</creatorcontrib><creatorcontrib>Coffy, Amandine</creatorcontrib><creatorcontrib>Daurès, Jean-Pierre</creatorcontrib><creatorcontrib>Maudelonde, Thierry</creatorcontrib><creatorcontrib>Fabre, Jean-Michel</creatorcontrib><creatorcontrib>Pantel, Klaus</creatorcontrib><creatorcontrib>Alix-Panabières, Catherine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Proquest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Denève, Eric</au><au>Riethdorf, Sabine</au><au>Ramos, Jeanne</au><au>Nocca, David</au><au>Coffy, Amandine</au><au>Daurès, Jean-Pierre</au><au>Maudelonde, Thierry</au><au>Fabre, Jean-Michel</au><au>Pantel, Klaus</au><au>Alix-Panabières, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capture of viable circulating tumor cells in the liver of colorectal cancer patients</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2013-09</date><risdate>2013</risdate><volume>59</volume><issue>9</issue><spage>1384</spage><epage>1392</epage><pages>1384-1392</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><abstract>The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.
We detected CTCs in the peripheral and mesenteric blood of colorectal cancer patients by use of 2 independent technologies on the basis of different biological properties of colon cancer cells. Seventy-five patients diagnosed with localized (M0, n = 60) and metastatic (M1, n = 15) colorectal cancer were included. Peripheral and mesenteric blood samples were collected before tumor resection. We performed CTC enumeration with an EpCAM-independent enrichment method followed by the Epispot assay that detected only viable CK19-releasing CTCs. In parallel, we used the FDA-cleared EpCAM-dependent CellSearch® as the reference method.
The enumeration of CK19-releasing cells by the CK19-Epispot assay revealed viable CTCs in 27 of 41 (65.9%) and 41 of 74 (55.4%) (P = 0.04) patients in mesenteric and peripheral blood, respectively, whereas CellSearch detected CTCs in 19 of 34 (55.9%) and 20 of 69 (29.0%) (P = 0.0046) patients. In mesenteric blood, medians of 4 (range 0-247) and 2.7 CTCs (range 0-286) were found with Epispot and CellSearch (P = 0.2), respectively, whereas in peripheral blood, Epispot and CellSearch detected a median of 1.2 (range 0-92) and 0 CTCs (range 0-147) (P = 0.002).
A considerable portion of viable CTCs detectable by the Epispot assay are trapped in the liver as the first filter organ in CRC patients.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>23695297</pmid><doi>10.1373/clinchem.2013.202846</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9147 |
| ispartof | Clinical chemistry (Baltimore, Md.), 2013-09, Vol.59 (9), p.1384-1392 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1429218905 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Aged Aged, 80 and over Agreements Biological properties Colon Colon - pathology Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - blood Colorectal Neoplasms - diagnosis Colorectal Neoplasms - pathology Female Humans Liver Liver - pathology Male Methods Middle Aged Neoplastic Cells, Circulating - pathology Prognosis Rectum - pathology Studies Surgery Tumors |
| title | Capture of viable circulating tumor cells in the liver of colorectal cancer patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T05%3A34%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Capture%20of%20viable%20circulating%20tumor%20cells%20in%20the%20liver%20of%20colorectal%20cancer%20patients&rft.jtitle=Clinical%20chemistry%20(Baltimore,%20Md.)&rft.au=Den%C3%A8ve,%20Eric&rft.date=2013-09&rft.volume=59&rft.issue=9&rft.spage=1384&rft.epage=1392&rft.pages=1384-1392&rft.issn=0009-9147&rft.eissn=1530-8561&rft_id=info:doi/10.1373/clinchem.2013.202846&rft_dat=%3Cproquest_cross%3E3109838781%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1445359545&rft_id=info:pmid/23695297&rfr_iscdi=true |