Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula
•A pectic polysaccharide (CPP1b) from Codonopsis pilosula was at first identified.•The chemical structure of CPP1b was elucidated.•The ultramicrostructure of CPP1b was described.•CPP1b presents potential anti-tumor and adjucant anti-tumor activity. A pectic polysaccharide (CPP1b) was at first isolat...
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| Veröffentlicht in: | Carbohydrate polymers 2013-10, Vol.98 (1), p.886-895 |
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| creator | Yang, Chunxia Gou, Yuqiang Chen, Jiayu An, Jing Chen, Wenxia Hu, Fangdi |
| description | •A pectic polysaccharide (CPP1b) from Codonopsis pilosula was at first identified.•The chemical structure of CPP1b was elucidated.•The ultramicrostructure of CPP1b was described.•CPP1b presents potential anti-tumor and adjucant anti-tumor activity.
A pectic polysaccharide (CPP1b) was at first isolated from Codonopsis pilosula. Sugar analysis revealed that CPP1b is composed of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molar ratio of 0.25:0.12:0.13:2.51. The result of esterification assay showed that about 46.7±0.4% of carboxylic groups in GalA residues existed as methyl ester. Combined with chemical and spectroscopic analyses, a preliminary structure of CPP1b was proposed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare 1,2-linked β-l-Rhap, 1,2,6-linked α-d-Galp and terminal α-l-Arap. CPP1b had an average molar mass and root-mean square radius (RMS) of 1.45×105Da and 29.7nm, respectively, and presented a linear random coil conformation in 0.9% NaCl. The ultrastructure of CPP1b was further investigated by transmission electron microscope (TEM) and scanning electron microscope (SEM). CPP1b exhibited obvious cytotoxicity to human lung adenocarcinoma A549 cells in a dose- and time-dependent manner. |
| doi_str_mv | 10.1016/j.carbpol.2013.06.079 |
| format | Article |
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A pectic polysaccharide (CPP1b) was at first isolated from Codonopsis pilosula. Sugar analysis revealed that CPP1b is composed of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molar ratio of 0.25:0.12:0.13:2.51. The result of esterification assay showed that about 46.7±0.4% of carboxylic groups in GalA residues existed as methyl ester. Combined with chemical and spectroscopic analyses, a preliminary structure of CPP1b was proposed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare 1,2-linked β-l-Rhap, 1,2,6-linked α-d-Galp and terminal α-l-Arap. CPP1b had an average molar mass and root-mean square radius (RMS) of 1.45×105Da and 29.7nm, respectively, and presented a linear random coil conformation in 0.9% NaCl. The ultrastructure of CPP1b was further investigated by transmission electron microscope (TEM) and scanning electron microscope (SEM). CPP1b exhibited obvious cytotoxicity to human lung adenocarcinoma A549 cells in a dose- and time-dependent manner.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2013.06.079</identifier><identifier>PMID: 23987425</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>adenocarcinoma ; Antineoplastic agents ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - isolation & purification ; Antineoplastic Agents - pharmacology ; Applied sciences ; arabinose ; Biological and medical sciences ; Cell Line, Tumor ; Chemotherapy ; Codonopsis - chemistry ; Codonopsis pilosula ; Cytotoxicity ; Esterification ; Exact sciences and technology ; galactose ; galacturonic acid ; GPC-MALLS ; Humans ; Medical sciences ; Methylation ; molecular weight ; Natural polymers ; NMR ; Pectic polysaccharide ; Pectins - chemistry ; Pectins - isolation & purification ; Pectins - pharmacology ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; polysaccharides ; rhamnose ; scanning electron microscopes ; scanning electron microscopy ; sodium chloride ; spectroscopy ; Starch and polysaccharides ; transmission electron microscopes ; transmission electron microscopy ; Ultrastructure</subject><ispartof>Carbohydrate polymers, 2013-10, Vol.98 (1), p.886-895</ispartof><rights>2013 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-a1c2d9039539fab1dbe81887cc493cbbd5d8505fd7942977987e3f0a1550b8ee3</citedby><cites>FETCH-LOGICAL-c485t-a1c2d9039539fab1dbe81887cc493cbbd5d8505fd7942977987e3f0a1550b8ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861713006759$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27757177$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23987425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Chunxia</creatorcontrib><creatorcontrib>Gou, Yuqiang</creatorcontrib><creatorcontrib>Chen, Jiayu</creatorcontrib><creatorcontrib>An, Jing</creatorcontrib><creatorcontrib>Chen, Wenxia</creatorcontrib><creatorcontrib>Hu, Fangdi</creatorcontrib><title>Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•A pectic polysaccharide (CPP1b) from Codonopsis pilosula was at first identified.•The chemical structure of CPP1b was elucidated.•The ultramicrostructure of CPP1b was described.•CPP1b presents potential anti-tumor and adjucant anti-tumor activity.
A pectic polysaccharide (CPP1b) was at first isolated from Codonopsis pilosula. Sugar analysis revealed that CPP1b is composed of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molar ratio of 0.25:0.12:0.13:2.51. The result of esterification assay showed that about 46.7±0.4% of carboxylic groups in GalA residues existed as methyl ester. Combined with chemical and spectroscopic analyses, a preliminary structure of CPP1b was proposed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare 1,2-linked β-l-Rhap, 1,2,6-linked α-d-Galp and terminal α-l-Arap. CPP1b had an average molar mass and root-mean square radius (RMS) of 1.45×105Da and 29.7nm, respectively, and presented a linear random coil conformation in 0.9% NaCl. The ultrastructure of CPP1b was further investigated by transmission electron microscope (TEM) and scanning electron microscope (SEM). CPP1b exhibited obvious cytotoxicity to human lung adenocarcinoma A549 cells in a dose- and time-dependent manner.</description><subject>adenocarcinoma</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - isolation & purification</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Applied sciences</subject><subject>arabinose</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Chemotherapy</subject><subject>Codonopsis - chemistry</subject><subject>Codonopsis pilosula</subject><subject>Cytotoxicity</subject><subject>Esterification</subject><subject>Exact sciences and technology</subject><subject>galactose</subject><subject>galacturonic acid</subject><subject>GPC-MALLS</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methylation</subject><subject>molecular weight</subject><subject>Natural polymers</subject><subject>NMR</subject><subject>Pectic polysaccharide</subject><subject>Pectins - chemistry</subject><subject>Pectins - isolation & purification</subject><subject>Pectins - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>polysaccharides</subject><subject>rhamnose</subject><subject>scanning electron microscopes</subject><subject>scanning electron microscopy</subject><subject>sodium chloride</subject><subject>spectroscopy</subject><subject>Starch and polysaccharides</subject><subject>transmission electron microscopes</subject><subject>transmission electron microscopy</subject><subject>Ultrastructure</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2P1CAUhonRuOPqT1C5MfGmFUopcGU2E7-STbxY95qc8qFM2lKBbjL-epnMqJeSEEJ4Xs7hAaGXlLSU0OHdoTWQxjVObUcoa8nQEqEeoR2VQjWU9f1jtCO07xs5UHGFnuV8IHUMlDxFVx1TUvQd3yF_V9JmypZgwuYHJDDFpfALSogLhsXWWULZ5phwPQoPoRxx9Bjw6urW4NrAMYM5RYN12Kc44320cYlrDhmvYYp5m-A5euJhyu7FZb1G9x8_fNt_bm6_fvqyv7ltTC95aYCazirCFGfKw0jt6CSVUhjTK2bG0XIrOeHeCtV3Soj6Csc8Aco5GaVz7Bq9Pd-7pvhzc7noOWTjpgkWF7esaY11VEhOK8rPqEkx5-S8XlOYIR01JfqkWB_0RbE-KdZk0FVxzb26lNjG2dm_qT9OK_DmAkA2MPkEiwn5HycEF1SIyr0-cx6ihu-pMvd3tdJQv4kzxmQl3p8JV5U9BJd0NsEtxtmQqn5tY_hPs78BLWqn_Q</recordid><startdate>20131015</startdate><enddate>20131015</enddate><creator>Yang, Chunxia</creator><creator>Gou, Yuqiang</creator><creator>Chen, Jiayu</creator><creator>An, Jing</creator><creator>Chen, Wenxia</creator><creator>Hu, Fangdi</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131015</creationdate><title>Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula</title><author>Yang, Chunxia ; Gou, Yuqiang ; Chen, Jiayu ; An, Jing ; Chen, Wenxia ; Hu, Fangdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-a1c2d9039539fab1dbe81887cc493cbbd5d8505fd7942977987e3f0a1550b8ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>adenocarcinoma</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - isolation & purification</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Applied sciences</topic><topic>arabinose</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Chemotherapy</topic><topic>Codonopsis - chemistry</topic><topic>Codonopsis pilosula</topic><topic>Cytotoxicity</topic><topic>Esterification</topic><topic>Exact sciences and technology</topic><topic>galactose</topic><topic>galacturonic acid</topic><topic>GPC-MALLS</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methylation</topic><topic>molecular weight</topic><topic>Natural polymers</topic><topic>NMR</topic><topic>Pectic polysaccharide</topic><topic>Pectins - chemistry</topic><topic>Pectins - isolation & purification</topic><topic>Pectins - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>polysaccharides</topic><topic>rhamnose</topic><topic>scanning electron microscopes</topic><topic>scanning electron microscopy</topic><topic>sodium chloride</topic><topic>spectroscopy</topic><topic>Starch and polysaccharides</topic><topic>transmission electron microscopes</topic><topic>transmission electron microscopy</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chunxia</creatorcontrib><creatorcontrib>Gou, Yuqiang</creatorcontrib><creatorcontrib>Chen, Jiayu</creatorcontrib><creatorcontrib>An, Jing</creatorcontrib><creatorcontrib>Chen, Wenxia</creatorcontrib><creatorcontrib>Hu, Fangdi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chunxia</au><au>Gou, Yuqiang</au><au>Chen, Jiayu</au><au>An, Jing</au><au>Chen, Wenxia</au><au>Hu, Fangdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2013-10-15</date><risdate>2013</risdate><volume>98</volume><issue>1</issue><spage>886</spage><epage>895</epage><pages>886-895</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>•A pectic polysaccharide (CPP1b) from Codonopsis pilosula was at first identified.•The chemical structure of CPP1b was elucidated.•The ultramicrostructure of CPP1b was described.•CPP1b presents potential anti-tumor and adjucant anti-tumor activity.
A pectic polysaccharide (CPP1b) was at first isolated from Codonopsis pilosula. Sugar analysis revealed that CPP1b is composed of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molar ratio of 0.25:0.12:0.13:2.51. The result of esterification assay showed that about 46.7±0.4% of carboxylic groups in GalA residues existed as methyl ester. Combined with chemical and spectroscopic analyses, a preliminary structure of CPP1b was proposed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare 1,2-linked β-l-Rhap, 1,2,6-linked α-d-Galp and terminal α-l-Arap. CPP1b had an average molar mass and root-mean square radius (RMS) of 1.45×105Da and 29.7nm, respectively, and presented a linear random coil conformation in 0.9% NaCl. The ultrastructure of CPP1b was further investigated by transmission electron microscope (TEM) and scanning electron microscope (SEM). CPP1b exhibited obvious cytotoxicity to human lung adenocarcinoma A549 cells in a dose- and time-dependent manner.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23987425</pmid><doi>10.1016/j.carbpol.2013.06.079</doi><tpages>10</tpages></addata></record> |
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| subjects | adenocarcinoma Antineoplastic agents Antineoplastic Agents - chemistry Antineoplastic Agents - isolation & purification Antineoplastic Agents - pharmacology Applied sciences arabinose Biological and medical sciences Cell Line, Tumor Chemotherapy Codonopsis - chemistry Codonopsis pilosula Cytotoxicity Esterification Exact sciences and technology galactose galacturonic acid GPC-MALLS Humans Medical sciences Methylation molecular weight Natural polymers NMR Pectic polysaccharide Pectins - chemistry Pectins - isolation & purification Pectins - pharmacology Pharmacology. Drug treatments Physicochemistry of polymers polysaccharides rhamnose scanning electron microscopes scanning electron microscopy sodium chloride spectroscopy Starch and polysaccharides transmission electron microscopes transmission electron microscopy Ultrastructure |
| title | Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula |
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