Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran
To investigate the prevalence of nine virulence and toxin genes of Campylobacter, a total of 90 Campylobacter strains including 48 C. jejuni and 42 C. coli were recovered from chicken feces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection...
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| Veröffentlicht in: | Foodborne pathogens and disease 2013-09, Vol.10 (9), p.764-770 |
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| creator | KHOSHBAKHT, Rahem TABATABAEI, Mohammad HOSSEINZADEH, Saeid SEIED SHAHRAM SHEKARFOROUSH HESAMADDIN SHIRZAD ASKI |
| description | To investigate the prevalence of nine virulence and toxin genes of Campylobacter, a total of 90 Campylobacter strains including 48 C. jejuni and 42 C. coli were recovered from chicken feces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two species. For confirmed strains, PCR was carried out for the presence of virulence genes using specific primers. Data were analyzed by SPSS software, version 12.0.1. The cadF gene and three genes associated with cytolethal distending toxin were present in 100% of isolates. Plasmid virB11 gene was not found in any of the Campylobacter isolates, and the prevalence of pldA, wlaN, iamA, and cgtB genes were 92.22%, 82.22%, 81.11%, and 22.22%, respectively. The findings revealed that the distribution of the majority of these genes were not dissimilar among Campylobacter species. The results emphasized that many of the pathogenic C. jejuni and C. coli may have these genes, and the Campylobacter strains with poultry origin have pathogenic potential properties for humans. |
| doi_str_mv | 10.1089/fpd.2013.1489 |
| format | Article |
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The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two species. For confirmed strains, PCR was carried out for the presence of virulence genes using specific primers. Data were analyzed by SPSS software, version 12.0.1. The cadF gene and three genes associated with cytolethal distending toxin were present in 100% of isolates. Plasmid virB11 gene was not found in any of the Campylobacter isolates, and the prevalence of pldA, wlaN, iamA, and cgtB genes were 92.22%, 82.22%, 81.11%, and 22.22%, respectively. The findings revealed that the distribution of the majority of these genes were not dissimilar among Campylobacter species. The results emphasized that many of the pathogenic C. jejuni and C. coli may have these genes, and the Campylobacter strains with poultry origin have pathogenic potential properties for humans.</description><identifier>ISSN: 1535-3141</identifier><identifier>EISSN: 1556-7125</identifier><identifier>DOI: 10.1089/fpd.2013.1489</identifier><identifier>PMID: 23789768</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Animal productions ; Animals ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Toxins - genetics ; Biological and medical sciences ; Campylobacter coli - genetics ; Campylobacter coli - isolation & purification ; Campylobacter coli - pathogenicity ; Campylobacter jejuni - genetics ; Campylobacter jejuni - isolation & purification ; Campylobacter jejuni - pathogenicity ; Carrier Proteins - genetics ; DNA Primers ; DNA, Bacterial - genetics ; Feces - microbiology ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; General aspects ; Genes, Bacterial ; Hygiene and safety ; Iran ; Meat - microbiology ; Polymerase Chain Reaction ; Poultry ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Terrestrial animal productions ; Vertebrates ; Virulence - genetics</subject><ispartof>Foodborne pathogens and disease, 2013-09, Vol.10 (9), p.764-770</ispartof><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-cdab88774499ec7e8526c415cfac6a8b8fae8245d411679818a2ecfd83b562253</citedby><cites>FETCH-LOGICAL-c323t-cdab88774499ec7e8526c415cfac6a8b8fae8245d411679818a2ecfd83b562253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27723181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23789768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KHOSHBAKHT, Rahem</creatorcontrib><creatorcontrib>TABATABAEI, Mohammad</creatorcontrib><creatorcontrib>HOSSEINZADEH, Saeid</creatorcontrib><creatorcontrib>SEIED SHAHRAM SHEKARFOROUSH</creatorcontrib><creatorcontrib>HESAMADDIN SHIRZAD ASKI</creatorcontrib><title>Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran</title><title>Foodborne pathogens and disease</title><addtitle>Foodborne Pathog Dis</addtitle><description>To investigate the prevalence of nine virulence and toxin genes of Campylobacter, a total of 90 Campylobacter strains including 48 C. jejuni and 42 C. coli were recovered from chicken feces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two species. For confirmed strains, PCR was carried out for the presence of virulence genes using specific primers. Data were analyzed by SPSS software, version 12.0.1. The cadF gene and three genes associated with cytolethal distending toxin were present in 100% of isolates. Plasmid virB11 gene was not found in any of the Campylobacter isolates, and the prevalence of pldA, wlaN, iamA, and cgtB genes were 92.22%, 82.22%, 81.11%, and 22.22%, respectively. The findings revealed that the distribution of the majority of these genes were not dissimilar among Campylobacter species. The results emphasized that many of the pathogenic C. jejuni and C. coli may have these genes, and the Campylobacter strains with poultry origin have pathogenic potential properties for humans.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>Biological and medical sciences</subject><subject>Campylobacter coli - genetics</subject><subject>Campylobacter coli - isolation & purification</subject><subject>Campylobacter coli - pathogenicity</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>Campylobacter jejuni - pathogenicity</subject><subject>Carrier Proteins - genetics</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - genetics</subject><subject>Feces - microbiology</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Genes, Bacterial</subject><subject>Hygiene and safety</subject><subject>Iran</subject><subject>Meat - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Poultry</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><subject>Virulence - genetics</subject><issn>1535-3141</issn><issn>1556-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0D1v2zAQgGEiSJGvZsxacAnQIXJ0pChSY-p81ECQDmmzChR1RGhQpEtKQ_oL-rMr10478YbnDuBLyAWUCyhVc203_YKVwBdQqeaAnIAQdSGBicPtzEXBoYJjcprzuixZw4Q8IseMS9XIWp2Q37cuj8l10-hioNHSJxeQvrg0eQwGi5uco3F6xJ4-YMBMXaBLPWzefOy0GTHRNa6n4KgOPV0uqIne0VWO_u-KTXGgX1J0fob3aHb7z68u6V9X9DlO4yumQFdJh4_kg9U-4_n-PSM_7u--L78Wj98eVsubx8JwxsfC9LpTSsqqaho0EpVgtalAGKtNrVWnrEbFKtFXALVsFCjN0Nhe8U7UjAl-Rj7v7m5S_DlhHtvBZYPe64Bxyi1UcySohSpnWuyoSTHnhLbdJDfo9NZC2W7jt3P8dhu_3caf_af96akbsP-n32vP4HIPdDba2_nbxuX_TkrGQQH_A2rbjbY</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>KHOSHBAKHT, Rahem</creator><creator>TABATABAEI, Mohammad</creator><creator>HOSSEINZADEH, Saeid</creator><creator>SEIED SHAHRAM SHEKARFOROUSH</creator><creator>HESAMADDIN SHIRZAD ASKI</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran</title><author>KHOSHBAKHT, Rahem ; TABATABAEI, Mohammad ; HOSSEINZADEH, Saeid ; SEIED SHAHRAM SHEKARFOROUSH ; HESAMADDIN SHIRZAD ASKI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-cdab88774499ec7e8526c415cfac6a8b8fae8245d411679818a2ecfd83b562253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>Biological and medical sciences</topic><topic>Campylobacter coli - genetics</topic><topic>Campylobacter coli - isolation & purification</topic><topic>Campylobacter coli - pathogenicity</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Campylobacter jejuni - pathogenicity</topic><topic>Carrier Proteins - genetics</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - genetics</topic><topic>Feces - microbiology</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Genes, Bacterial</topic><topic>Hygiene and safety</topic><topic>Iran</topic><topic>Meat - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Poultry</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KHOSHBAKHT, Rahem</creatorcontrib><creatorcontrib>TABATABAEI, Mohammad</creatorcontrib><creatorcontrib>HOSSEINZADEH, Saeid</creatorcontrib><creatorcontrib>SEIED SHAHRAM SHEKARFOROUSH</creatorcontrib><creatorcontrib>HESAMADDIN SHIRZAD ASKI</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Foodborne pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KHOSHBAKHT, Rahem</au><au>TABATABAEI, Mohammad</au><au>HOSSEINZADEH, Saeid</au><au>SEIED SHAHRAM SHEKARFOROUSH</au><au>HESAMADDIN SHIRZAD ASKI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran</atitle><jtitle>Foodborne pathogens and disease</jtitle><addtitle>Foodborne Pathog Dis</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>10</volume><issue>9</issue><spage>764</spage><epage>770</epage><pages>764-770</pages><issn>1535-3141</issn><eissn>1556-7125</eissn><abstract>To investigate the prevalence of nine virulence and toxin genes of Campylobacter, a total of 90 Campylobacter strains including 48 C. jejuni and 42 C. coli were recovered from chicken feces by cultivation methods. The isolates were identified on the basis of polymerase chain reaction (PCR) detection of 16SrRNA and multiplex PCR for determining two species. For confirmed strains, PCR was carried out for the presence of virulence genes using specific primers. Data were analyzed by SPSS software, version 12.0.1. The cadF gene and three genes associated with cytolethal distending toxin were present in 100% of isolates. Plasmid virB11 gene was not found in any of the Campylobacter isolates, and the prevalence of pldA, wlaN, iamA, and cgtB genes were 92.22%, 82.22%, 81.11%, and 22.22%, respectively. The findings revealed that the distribution of the majority of these genes were not dissimilar among Campylobacter species. 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| subjects | Animal productions Animals Bacterial Outer Membrane Proteins - genetics Bacterial Toxins - genetics Biological and medical sciences Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter coli - pathogenicity Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification Campylobacter jejuni - pathogenicity Carrier Proteins - genetics DNA Primers DNA, Bacterial - genetics Feces - microbiology Food Contamination - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology General aspects Genes, Bacterial Hygiene and safety Iran Meat - microbiology Polymerase Chain Reaction Poultry RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Terrestrial animal productions Vertebrates Virulence - genetics |
| title | Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran |
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