Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer
Background There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment. Methods Faecal samples from 8...
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| Veröffentlicht in: | Journal of gastrointestinal surgery 2013-09, Vol.17 (9), p.1657-1664 |
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| creator | Ohigashi, Seiji Sudo, Kazuki Kobayashi, Daiki Takahashi, Takuya Nomoto, Koji Onodera, Hisashi |
| description | Background
There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.
Methods
Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.
Results
Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log
10
cells/g;
p
|
| doi_str_mv | 10.1007/s11605-013-2270-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1428513386</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3055201221</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-8b31d461ba5c4e60b0e1697f5a07024a84adc22039f875580d4af9f0612a93643</originalsourceid><addsrcrecordid>eNp1kU9r3DAQxUVpaP60H6CXYuilFzczkizJx2A2TWAhgSSQm5C98q4Wr5xKcrL59tVmk1IKOY1gfu_NaB4hXxF-IoA8jYgCqhKQlZRKKLcfyBEqyUouqPiY31BjSavq_pAcx7gGQAmoPpFDyhRICfSItDdu6V3vOuNT0ayMX9pYOF-klS0ufbIxOW-GYuYfXRj9xmbqrE82FDdTWNrwvGOvTXK5EYsnl1ZFMw5jsF3Kqsb4zobP5KA3Q7RfXusJuTuf3TYX5fzq12VzNi87JmkqVctwwQW2puq4FdCCRVHLvjKQN-VGcbPoKAVW90pWlYIFN33dg0BqaiY4OyE_9r4PYfw95c31xsXODoPxdpyiRk5VhYwpkdHv_6HrcQr5oy-UrGvBa8wU7qkujDEG2-uH4DYmPGsEvQtA7wPQOQC9C0Bvs-bbq_PUbuzir-Lt4hmgeyDmVr52-Gf0u65_ACzvkCo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1427996491</pqid></control><display><type>article</type><title>Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ohigashi, Seiji ; Sudo, Kazuki ; Kobayashi, Daiki ; Takahashi, Takuya ; Nomoto, Koji ; Onodera, Hisashi</creator><creatorcontrib>Ohigashi, Seiji ; Sudo, Kazuki ; Kobayashi, Daiki ; Takahashi, Takuya ; Nomoto, Koji ; Onodera, Hisashi</creatorcontrib><description>Background
There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.
Methods
Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.
Results
Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log
10
cells/g;
p
< 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of
Enterobacteriaceae
,
Enterococcus
,
Staphylococcus
and
Pseudomonas
were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g;
p
< 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed.
Conclusion
Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.</description><identifier>ISSN: 1091-255X</identifier><identifier>EISSN: 1873-4626</identifier><identifier>DOI: 10.1007/s11605-013-2270-x</identifier><identifier>PMID: 23807702</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antibiotics ; Bacterial Load ; Biomarkers - metabolism ; Carboxylic Acids - metabolism ; Case-Control Studies ; Chromatography, High Pressure Liquid ; Colectomy ; Colorectal cancer ; Colorectal Neoplasms - microbiology ; Colorectal Neoplasms - surgery ; Feces - chemistry ; Feces - microbiology ; Female ; Gastroenterology ; Hospitals ; Humans ; Hydrogen-Ion Concentration ; Male ; Medicine ; Medicine & Public Health ; Microbiota ; Middle Aged ; Original Article ; Ostomy ; Patients ; Postoperative Period ; Preoperative Period ; Prospective Studies ; Rectum - surgery ; Reverse Transcriptase Polymerase Chain Reaction ; Surgery ; Surgical site infections ; Surgical Wound Infection - etiology ; Treatment Outcome</subject><ispartof>Journal of gastrointestinal surgery, 2013-09, Vol.17 (9), p.1657-1664</ispartof><rights>The Society for Surgery of the Alimentary Tract 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-8b31d461ba5c4e60b0e1697f5a07024a84adc22039f875580d4af9f0612a93643</citedby><cites>FETCH-LOGICAL-c372t-8b31d461ba5c4e60b0e1697f5a07024a84adc22039f875580d4af9f0612a93643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11605-013-2270-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11605-013-2270-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23807702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohigashi, Seiji</creatorcontrib><creatorcontrib>Sudo, Kazuki</creatorcontrib><creatorcontrib>Kobayashi, Daiki</creatorcontrib><creatorcontrib>Takahashi, Takuya</creatorcontrib><creatorcontrib>Nomoto, Koji</creatorcontrib><creatorcontrib>Onodera, Hisashi</creatorcontrib><title>Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer</title><title>Journal of gastrointestinal surgery</title><addtitle>J Gastrointest Surg</addtitle><addtitle>J Gastrointest Surg</addtitle><description>Background
There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.
Methods
Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.
Results
Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log
10
cells/g;
p
< 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of
Enterobacteriaceae
,
Enterococcus
,
Staphylococcus
and
Pseudomonas
were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g;
p
< 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed.
Conclusion
Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibiotics</subject><subject>Bacterial Load</subject><subject>Biomarkers - metabolism</subject><subject>Carboxylic Acids - metabolism</subject><subject>Case-Control Studies</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Colectomy</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - microbiology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microbiota</subject><subject>Middle Aged</subject><subject>Original Article</subject><subject>Ostomy</subject><subject>Patients</subject><subject>Postoperative Period</subject><subject>Preoperative Period</subject><subject>Prospective Studies</subject><subject>Rectum - surgery</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Surgery</subject><subject>Surgical site infections</subject><subject>Surgical Wound Infection - etiology</subject><subject>Treatment Outcome</subject><issn>1091-255X</issn><issn>1873-4626</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kU9r3DAQxUVpaP60H6CXYuilFzczkizJx2A2TWAhgSSQm5C98q4Wr5xKcrL59tVmk1IKOY1gfu_NaB4hXxF-IoA8jYgCqhKQlZRKKLcfyBEqyUouqPiY31BjSavq_pAcx7gGQAmoPpFDyhRICfSItDdu6V3vOuNT0ayMX9pYOF-klS0ufbIxOW-GYuYfXRj9xmbqrE82FDdTWNrwvGOvTXK5EYsnl1ZFMw5jsF3Kqsb4zobP5KA3Q7RfXusJuTuf3TYX5fzq12VzNi87JmkqVctwwQW2puq4FdCCRVHLvjKQN-VGcbPoKAVW90pWlYIFN33dg0BqaiY4OyE_9r4PYfw95c31xsXODoPxdpyiRk5VhYwpkdHv_6HrcQr5oy-UrGvBa8wU7qkujDEG2-uH4DYmPGsEvQtA7wPQOQC9C0Bvs-bbq_PUbuzir-Lt4hmgeyDmVr52-Gf0u65_ACzvkCo</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Ohigashi, Seiji</creator><creator>Sudo, Kazuki</creator><creator>Kobayashi, Daiki</creator><creator>Takahashi, Takuya</creator><creator>Nomoto, Koji</creator><creator>Onodera, Hisashi</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer</title><author>Ohigashi, Seiji ; Sudo, Kazuki ; Kobayashi, Daiki ; Takahashi, Takuya ; Nomoto, Koji ; Onodera, Hisashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-8b31d461ba5c4e60b0e1697f5a07024a84adc22039f875580d4af9f0612a93643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antibiotics</topic><topic>Bacterial Load</topic><topic>Biomarkers - metabolism</topic><topic>Carboxylic Acids - metabolism</topic><topic>Case-Control Studies</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Colectomy</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - microbiology</topic><topic>Colorectal Neoplasms - surgery</topic><topic>Feces - chemistry</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microbiota</topic><topic>Middle Aged</topic><topic>Original Article</topic><topic>Ostomy</topic><topic>Patients</topic><topic>Postoperative Period</topic><topic>Preoperative Period</topic><topic>Prospective Studies</topic><topic>Rectum - surgery</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Surgery</topic><topic>Surgical site infections</topic><topic>Surgical Wound Infection - etiology</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohigashi, Seiji</creatorcontrib><creatorcontrib>Sudo, Kazuki</creatorcontrib><creatorcontrib>Kobayashi, Daiki</creatorcontrib><creatorcontrib>Takahashi, Takuya</creatorcontrib><creatorcontrib>Nomoto, Koji</creatorcontrib><creatorcontrib>Onodera, Hisashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastrointestinal surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohigashi, Seiji</au><au>Sudo, Kazuki</au><au>Kobayashi, Daiki</au><au>Takahashi, Takuya</au><au>Nomoto, Koji</au><au>Onodera, Hisashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer</atitle><jtitle>Journal of gastrointestinal surgery</jtitle><stitle>J Gastrointest Surg</stitle><addtitle>J Gastrointest Surg</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>17</volume><issue>9</issue><spage>1657</spage><epage>1664</epage><pages>1657-1664</pages><issn>1091-255X</issn><eissn>1873-4626</eissn><abstract>Background
There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.
Methods
Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.
Results
Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log
10
cells/g;
p
< 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of
Enterobacteriaceae
,
Enterococcus
,
Staphylococcus
and
Pseudomonas
were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g;
p
< 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed.
Conclusion
Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23807702</pmid><doi>10.1007/s11605-013-2270-x</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Antibiotics Bacterial Load Biomarkers - metabolism Carboxylic Acids - metabolism Case-Control Studies Chromatography, High Pressure Liquid Colectomy Colorectal cancer Colorectal Neoplasms - microbiology Colorectal Neoplasms - surgery Feces - chemistry Feces - microbiology Female Gastroenterology Hospitals Humans Hydrogen-Ion Concentration Male Medicine Medicine & Public Health Microbiota Middle Aged Original Article Ostomy Patients Postoperative Period Preoperative Period Prospective Studies Rectum - surgery Reverse Transcriptase Polymerase Chain Reaction Surgery Surgical site infections Surgical Wound Infection - etiology Treatment Outcome |
| title | Significant Changes in the Intestinal Environment After Surgery in Patients with Colorectal Cancer |
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