Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells
Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence....
Gespeichert in:
| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1985-06, Vol.82 (11), p.3884-3888 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3888 |
|---|---|
| container_issue | 11 |
| container_start_page | 3884 |
| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
| container_volume | 82 |
| creator | Lechner, John F. Tokiwa, Takayoshi LaVeck, Moira Benedict, William F. Banks-Schlegel, Susan Yeager, Henry Banerjee, Asutosh Harris, Curtis C. |
| description | Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells. |
| doi_str_mv | 10.1073/pnas.82.11.3884 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_14284079</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>25775</jstor_id><sourcerecordid>25775</sourcerecordid><originalsourceid>FETCH-LOGICAL-c519t-ccf50de386e1cb91a637063d7be40bf5d375957d1b82765acfd160b1923619323</originalsourceid><addsrcrecordid>eNp9kc1v1DAQxS0EKkvhjIQEWnGAU7b-iGP70MOyAopUxAXOluNMuq4Se8k4CP57HO1S6IWTLb3fmzf2I-Q5oxtGlbg4RIcbzTeMbYTW9QOyYtSwqqkNfUhWlHJV6ZrXj8kTxFtKqZGanpEzbrQykq_Iuy22gDlhtUVMPrgM3Xq3n9KYMI1uKHcXbwDXIa6v5tHF9WfAlPcwhEWEYcCn5FHvBoRnp_OcfPvw_uvuqrr-8vHTbntdeclMrrzvJe1A6AaYbw1zjVC0EZ1qoaZtLzuhpJGqY63mqpHO9x1raMsMFw0zgotzcnmce5jbEToPMU9usIcpjG76ZZML9r4Sw97epB9WGKWNKP7XR3_CHCz6kMHvfYoRfLYN1yVdFujNKWRK3-fyM3YM6MszXYQ0o2U11zVVpoAXR9BPCXGC_m4RRu1SjV2qsZpbxuxSTXG8_Hf_O_7URdFfnfTF-Ee9N-DtfwHbz8OQ4Wcu5IsjeVuqnf4mSaWk-A0OoKuD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14284079</pqid></control><display><type>article</type><title>Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Lechner, John F. ; Tokiwa, Takayoshi ; LaVeck, Moira ; Benedict, William F. ; Banks-Schlegel, Susan ; Yeager, Henry ; Banerjee, Asutosh ; Harris, Curtis C.</creator><creatorcontrib>Lechner, John F. ; Tokiwa, Takayoshi ; LaVeck, Moira ; Benedict, William F. ; Banks-Schlegel, Susan ; Yeager, Henry ; Banerjee, Asutosh ; Harris, Curtis C. ; National Institutes of Health, Bethesda, MD</creatorcontrib><description>Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.11.3884</identifier><identifier>PMID: 2987952</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>560301 - Chemicals Metabolism & Toxicology- Cells- (-1987) ; ANEUPLOIDY ; ANIMAL CELLS ; ANIMALS ; ASBESTOS ; Asbestos - toxicity ; Asbestos, Serpentine ; BIOLOGICAL EFFECTS ; BODY ; CARCINOGENESIS ; CELL CULTURES ; Cell growth ; Chromosomes ; Chromosomes, Human ; Cultured cells ; ELECTRON MICROSCOPY ; Epithelial cells ; GENETIC EFFECTS ; Humans ; Karyotyping ; Keratins ; Lung - ultrastructure ; Lung Neoplasms - genetics ; Lung Neoplasms - ultrastructure ; LUNGS ; MAMMALS ; MAN ; Mesothelioma ; Mesothelioma - genetics ; Mesothelioma - ultrastructure ; Metaphase ; MICROSCOPY ; Microscopy, Electron, Scanning ; ORGANS ; PATHOGENESIS ; Phagocytosis ; Pleural Effusion ; PLOIDY ; PRIMATES ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RESPIRATORY SYSTEM ; RESPIRATORY TRACT CELLS ; SCANNING ELECTRON MICROSCOPY ; VERTEBRATES</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-06, Vol.82 (11), p.3884-3888</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-ccf50de386e1cb91a637063d7be40bf5d375957d1b82765acfd160b1923619323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/11.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25775$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25775$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2987952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6285955$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Lechner, John F.</creatorcontrib><creatorcontrib>Tokiwa, Takayoshi</creatorcontrib><creatorcontrib>LaVeck, Moira</creatorcontrib><creatorcontrib>Benedict, William F.</creatorcontrib><creatorcontrib>Banks-Schlegel, Susan</creatorcontrib><creatorcontrib>Yeager, Henry</creatorcontrib><creatorcontrib>Banerjee, Asutosh</creatorcontrib><creatorcontrib>Harris, Curtis C.</creatorcontrib><creatorcontrib>National Institutes of Health, Bethesda, MD</creatorcontrib><title>Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.</description><subject>560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)</subject><subject>ANEUPLOIDY</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>ASBESTOS</subject><subject>Asbestos - toxicity</subject><subject>Asbestos, Serpentine</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BODY</subject><subject>CARCINOGENESIS</subject><subject>CELL CULTURES</subject><subject>Cell growth</subject><subject>Chromosomes</subject><subject>Chromosomes, Human</subject><subject>Cultured cells</subject><subject>ELECTRON MICROSCOPY</subject><subject>Epithelial cells</subject><subject>GENETIC EFFECTS</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Keratins</subject><subject>Lung - ultrastructure</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - ultrastructure</subject><subject>LUNGS</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Mesothelioma</subject><subject>Mesothelioma - genetics</subject><subject>Mesothelioma - ultrastructure</subject><subject>Metaphase</subject><subject>MICROSCOPY</subject><subject>Microscopy, Electron, Scanning</subject><subject>ORGANS</subject><subject>PATHOGENESIS</subject><subject>Phagocytosis</subject><subject>Pleural Effusion</subject><subject>PLOIDY</subject><subject>PRIMATES</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RESPIRATORY SYSTEM</subject><subject>RESPIRATORY TRACT CELLS</subject><subject>SCANNING ELECTRON MICROSCOPY</subject><subject>VERTEBRATES</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS0EKkvhjIQEWnGAU7b-iGP70MOyAopUxAXOluNMuq4Se8k4CP57HO1S6IWTLb3fmzf2I-Q5oxtGlbg4RIcbzTeMbYTW9QOyYtSwqqkNfUhWlHJV6ZrXj8kTxFtKqZGanpEzbrQykq_Iuy22gDlhtUVMPrgM3Xq3n9KYMI1uKHcXbwDXIa6v5tHF9WfAlPcwhEWEYcCn5FHvBoRnp_OcfPvw_uvuqrr-8vHTbntdeclMrrzvJe1A6AaYbw1zjVC0EZ1qoaZtLzuhpJGqY63mqpHO9x1raMsMFw0zgotzcnmce5jbEToPMU9usIcpjG76ZZML9r4Sw97epB9WGKWNKP7XR3_CHCz6kMHvfYoRfLYN1yVdFujNKWRK3-fyM3YM6MszXYQ0o2U11zVVpoAXR9BPCXGC_m4RRu1SjV2qsZpbxuxSTXG8_Hf_O_7URdFfnfTF-Ee9N-DtfwHbz8OQ4Wcu5IsjeVuqnf4mSaWk-A0OoKuD</recordid><startdate>19850601</startdate><enddate>19850601</enddate><creator>Lechner, John F.</creator><creator>Tokiwa, Takayoshi</creator><creator>LaVeck, Moira</creator><creator>Benedict, William F.</creator><creator>Banks-Schlegel, Susan</creator><creator>Yeager, Henry</creator><creator>Banerjee, Asutosh</creator><creator>Harris, Curtis C.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19850601</creationdate><title>Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells</title><author>Lechner, John F. ; Tokiwa, Takayoshi ; LaVeck, Moira ; Benedict, William F. ; Banks-Schlegel, Susan ; Yeager, Henry ; Banerjee, Asutosh ; Harris, Curtis C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-ccf50de386e1cb91a637063d7be40bf5d375957d1b82765acfd160b1923619323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)</topic><topic>ANEUPLOIDY</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>ASBESTOS</topic><topic>Asbestos - toxicity</topic><topic>Asbestos, Serpentine</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BODY</topic><topic>CARCINOGENESIS</topic><topic>CELL CULTURES</topic><topic>Cell growth</topic><topic>Chromosomes</topic><topic>Chromosomes, Human</topic><topic>Cultured cells</topic><topic>ELECTRON MICROSCOPY</topic><topic>Epithelial cells</topic><topic>GENETIC EFFECTS</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Keratins</topic><topic>Lung - ultrastructure</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - ultrastructure</topic><topic>LUNGS</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Mesothelioma</topic><topic>Mesothelioma - genetics</topic><topic>Mesothelioma - ultrastructure</topic><topic>Metaphase</topic><topic>MICROSCOPY</topic><topic>Microscopy, Electron, Scanning</topic><topic>ORGANS</topic><topic>PATHOGENESIS</topic><topic>Phagocytosis</topic><topic>Pleural Effusion</topic><topic>PLOIDY</topic><topic>PRIMATES</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RESPIRATORY SYSTEM</topic><topic>RESPIRATORY TRACT CELLS</topic><topic>SCANNING ELECTRON MICROSCOPY</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lechner, John F.</creatorcontrib><creatorcontrib>Tokiwa, Takayoshi</creatorcontrib><creatorcontrib>LaVeck, Moira</creatorcontrib><creatorcontrib>Benedict, William F.</creatorcontrib><creatorcontrib>Banks-Schlegel, Susan</creatorcontrib><creatorcontrib>Yeager, Henry</creatorcontrib><creatorcontrib>Banerjee, Asutosh</creatorcontrib><creatorcontrib>Harris, Curtis C.</creatorcontrib><creatorcontrib>National Institutes of Health, Bethesda, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lechner, John F.</au><au>Tokiwa, Takayoshi</au><au>LaVeck, Moira</au><au>Benedict, William F.</au><au>Banks-Schlegel, Susan</au><au>Yeager, Henry</au><au>Banerjee, Asutosh</au><au>Harris, Curtis C.</au><aucorp>National Institutes of Health, Bethesda, MD</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-06-01</date><risdate>1985</risdate><volume>82</volume><issue>11</issue><spage>3884</spage><epage>3888</epage><pages>3884-3888</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2987952</pmid><doi>10.1073/pnas.82.11.3884</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-06, Vol.82 (11), p.3884-3888 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14284079 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987) ANEUPLOIDY ANIMAL CELLS ANIMALS ASBESTOS Asbestos - toxicity Asbestos, Serpentine BIOLOGICAL EFFECTS BODY CARCINOGENESIS CELL CULTURES Cell growth Chromosomes Chromosomes, Human Cultured cells ELECTRON MICROSCOPY Epithelial cells GENETIC EFFECTS Humans Karyotyping Keratins Lung - ultrastructure Lung Neoplasms - genetics Lung Neoplasms - ultrastructure LUNGS MAMMALS MAN Mesothelioma Mesothelioma - genetics Mesothelioma - ultrastructure Metaphase MICROSCOPY Microscopy, Electron, Scanning ORGANS PATHOGENESIS Phagocytosis Pleural Effusion PLOIDY PRIMATES RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RESPIRATORY SYSTEM RESPIRATORY TRACT CELLS SCANNING ELECTRON MICROSCOPY VERTEBRATES |
| title | Asbestos-Associated Chromosomal Changes in Human Mesothelial Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T03%3A09%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Asbestos-Associated%20Chromosomal%20Changes%20in%20Human%20Mesothelial%20Cells&rft.jtitle=Proc.%20Natl.%20Acad.%20Sci.%20U.S.A.;%20(United%20States)&rft.au=Lechner,%20John%20F.&rft.aucorp=National%20Institutes%20of%20Health,%20Bethesda,%20MD&rft.date=1985-06-01&rft.volume=82&rft.issue=11&rft.spage=3884&rft.epage=3888&rft.pages=3884-3888&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.82.11.3884&rft_dat=%3Cjstor_proqu%3E25775%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14284079&rft_id=info:pmid/2987952&rft_jstor_id=25775&rfr_iscdi=true |