Synergistic activation of lipopolysaccharide-stimulated glial cells by propofol
•Propofol synergistically increased NO and ROS production in LPS-stimulated glial cells.•Propofol also increased the expression of iNOS, MMP-9, IL-6 and IL-1β.•The effects were mediated by JNK and p38 activation.•The activated microglial cells may induce neuronal cell death. Despite the extensive us...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2013-08, Vol.438 (2), p.420-426 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Ko, Hyun Myung Kim, So Yeon Joo, So Hyun Cheong, Jae Hoon Yang, Sung-Il Shin, Chan Young Koo, Bon Nyeo |
| description | •Propofol synergistically increased NO and ROS production in LPS-stimulated glial cells.•Propofol also increased the expression of iNOS, MMP-9, IL-6 and IL-1β.•The effects were mediated by JNK and p38 activation.•The activated microglial cells may induce neuronal cell death.
Despite the extensive use of propofol in general anesthetic procedures, the effects of propofol on glial cell were not completely understood. In lipopolysaccharide (LPS)-stimulated rat primary astrocytes and BV2 microglial cell lines, co-treatment of propofol synergistically induced inflammatory activation as evidenced by the increased production of NO, ROS and expression of iNOS, MMP-9 and several cytokines. Propofol augmented the activation of JNK and p38 MAPKs induced by LPS and the synergistic activation of glial cells by propofol was prevented by pretreatment of JNK and p38 inhibitors. When we treated BV2 cell culture supernatants treated with LPS plus propofol on cultured rat primary neuron, it induced a significant neuronal cell death. The results suggest that the repeated use of propofol in immunologically challenged situation may induce glial activation in brain. |
| doi_str_mv | 10.1016/j.bbrc.2013.07.089 |
| format | Article |
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Despite the extensive use of propofol in general anesthetic procedures, the effects of propofol on glial cell were not completely understood. In lipopolysaccharide (LPS)-stimulated rat primary astrocytes and BV2 microglial cell lines, co-treatment of propofol synergistically induced inflammatory activation as evidenced by the increased production of NO, ROS and expression of iNOS, MMP-9 and several cytokines. Propofol augmented the activation of JNK and p38 MAPKs induced by LPS and the synergistic activation of glial cells by propofol was prevented by pretreatment of JNK and p38 inhibitors. When we treated BV2 cell culture supernatants treated with LPS plus propofol on cultured rat primary neuron, it induced a significant neuronal cell death. The results suggest that the repeated use of propofol in immunologically challenged situation may induce glial activation in brain.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2013.07.089</identifier><identifier>PMID: 23899524</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anesthetics, Intravenous - pharmacology ; Animals ; Astrocytes - cytology ; Cell Death ; Cell Survival ; Cells, Cultured ; Cerebral Cortex - metabolism ; Cytokines ; Cytokines - metabolism ; Dose-Response Relationship, Drug ; iNOS ; Interleukin-1beta - metabolism ; Interleukin-6 - metabolism ; JNK ; Lipopolysaccharides - metabolism ; MAP Kinase Kinase 4 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; Neuroglia - cytology ; Neuroglia - drug effects ; Neuronal death ; Neurons - cytology ; Neurons - metabolism ; Nitric Oxide - metabolism ; Nitric Oxide Synthase Type II - metabolism ; p38 ; p38 Mitogen-Activated Protein Kinases - metabolism ; Propofol - pharmacology ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species</subject><ispartof>Biochemical and biophysical research communications, 2013-08, Vol.438 (2), p.420-426</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-630c4152033fd848d3fdeb760ff5dcab61f02a9ccab188f9b9b54e4f80d15ed33</citedby><cites>FETCH-LOGICAL-c356t-630c4152033fd848d3fdeb760ff5dcab61f02a9ccab188f9b9b54e4f80d15ed33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2013.07.089$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23899524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ko, Hyun Myung</creatorcontrib><creatorcontrib>Kim, So Yeon</creatorcontrib><creatorcontrib>Joo, So Hyun</creatorcontrib><creatorcontrib>Cheong, Jae Hoon</creatorcontrib><creatorcontrib>Yang, Sung-Il</creatorcontrib><creatorcontrib>Shin, Chan Young</creatorcontrib><creatorcontrib>Koo, Bon Nyeo</creatorcontrib><title>Synergistic activation of lipopolysaccharide-stimulated glial cells by propofol</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•Propofol synergistically increased NO and ROS production in LPS-stimulated glial cells.•Propofol also increased the expression of iNOS, MMP-9, IL-6 and IL-1β.•The effects were mediated by JNK and p38 activation.•The activated microglial cells may induce neuronal cell death.
Despite the extensive use of propofol in general anesthetic procedures, the effects of propofol on glial cell were not completely understood. In lipopolysaccharide (LPS)-stimulated rat primary astrocytes and BV2 microglial cell lines, co-treatment of propofol synergistically induced inflammatory activation as evidenced by the increased production of NO, ROS and expression of iNOS, MMP-9 and several cytokines. Propofol augmented the activation of JNK and p38 MAPKs induced by LPS and the synergistic activation of glial cells by propofol was prevented by pretreatment of JNK and p38 inhibitors. When we treated BV2 cell culture supernatants treated with LPS plus propofol on cultured rat primary neuron, it induced a significant neuronal cell death. The results suggest that the repeated use of propofol in immunologically challenged situation may induce glial activation in brain.</description><subject>Anesthetics, Intravenous - pharmacology</subject><subject>Animals</subject><subject>Astrocytes - cytology</subject><subject>Cell Death</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>iNOS</subject><subject>Interleukin-1beta - metabolism</subject><subject>Interleukin-6 - metabolism</subject><subject>JNK</subject><subject>Lipopolysaccharides - metabolism</subject><subject>MAP Kinase Kinase 4 - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - drug effects</subject><subject>Neuronal death</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>p38</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Propofol - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reactive Oxygen Species</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlb_gAfZo5ddJ9nPgBcpfkGhBxW8hWwyqSlptybbwv57U1o9epo5PPPOzEPINYWMAq3ullnbepUxoHkGdQYNPyFjChxSRqE4JWMAqFLG6eeIXISwBKC0qPg5GbG84bxkxZjM34Y1-oUNvVWJVL3dyd5266QzibObbtO5IUilvqS3GtNIrbZO9qiThbPSJQqdC0k7JBsfWdO5S3JmpAt4dawT8vH0-D59SWfz59fpwyxVeVn1aZWDKmjJIM-NbopGx4JtXYExpVayragBJrmKLW0aw1velgUWpgFNS9R5PiG3h9y4-HuLoRcrG_bXyDV22yBowRpW05rVEWUHVPkuBI9GbLxdST8ICmIvUizFXqTYixRQiygyDt0c87ftCvXfyK-5CNwfAIxf7ix6EZTFtUJtPape6M7-l_8DMC6GRQ</recordid><startdate>20130823</startdate><enddate>20130823</enddate><creator>Ko, Hyun Myung</creator><creator>Kim, So Yeon</creator><creator>Joo, So Hyun</creator><creator>Cheong, Jae Hoon</creator><creator>Yang, Sung-Il</creator><creator>Shin, Chan Young</creator><creator>Koo, Bon Nyeo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130823</creationdate><title>Synergistic activation of lipopolysaccharide-stimulated glial cells by propofol</title><author>Ko, Hyun Myung ; Kim, So Yeon ; Joo, So Hyun ; Cheong, Jae Hoon ; Yang, Sung-Il ; Shin, Chan Young ; Koo, Bon Nyeo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-630c4152033fd848d3fdeb760ff5dcab61f02a9ccab188f9b9b54e4f80d15ed33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anesthetics, Intravenous - pharmacology</topic><topic>Animals</topic><topic>Astrocytes - cytology</topic><topic>Cell Death</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>iNOS</topic><topic>Interleukin-1beta - metabolism</topic><topic>Interleukin-6 - metabolism</topic><topic>JNK</topic><topic>Lipopolysaccharides - metabolism</topic><topic>MAP Kinase Kinase 4 - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - drug effects</topic><topic>Neuronal death</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>p38</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Propofol - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reactive Oxygen Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ko, Hyun Myung</creatorcontrib><creatorcontrib>Kim, So Yeon</creatorcontrib><creatorcontrib>Joo, So Hyun</creatorcontrib><creatorcontrib>Cheong, Jae Hoon</creatorcontrib><creatorcontrib>Yang, Sung-Il</creatorcontrib><creatorcontrib>Shin, Chan Young</creatorcontrib><creatorcontrib>Koo, Bon Nyeo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ko, Hyun Myung</au><au>Kim, So Yeon</au><au>Joo, So Hyun</au><au>Cheong, Jae Hoon</au><au>Yang, Sung-Il</au><au>Shin, Chan Young</au><au>Koo, Bon Nyeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic activation of lipopolysaccharide-stimulated glial cells by propofol</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2013-08-23</date><risdate>2013</risdate><volume>438</volume><issue>2</issue><spage>420</spage><epage>426</epage><pages>420-426</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•Propofol synergistically increased NO and ROS production in LPS-stimulated glial cells.•Propofol also increased the expression of iNOS, MMP-9, IL-6 and IL-1β.•The effects were mediated by JNK and p38 activation.•The activated microglial cells may induce neuronal cell death.
Despite the extensive use of propofol in general anesthetic procedures, the effects of propofol on glial cell were not completely understood. In lipopolysaccharide (LPS)-stimulated rat primary astrocytes and BV2 microglial cell lines, co-treatment of propofol synergistically induced inflammatory activation as evidenced by the increased production of NO, ROS and expression of iNOS, MMP-9 and several cytokines. Propofol augmented the activation of JNK and p38 MAPKs induced by LPS and the synergistic activation of glial cells by propofol was prevented by pretreatment of JNK and p38 inhibitors. When we treated BV2 cell culture supernatants treated with LPS plus propofol on cultured rat primary neuron, it induced a significant neuronal cell death. The results suggest that the repeated use of propofol in immunologically challenged situation may induce glial activation in brain.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23899524</pmid><doi>10.1016/j.bbrc.2013.07.089</doi><tpages>7</tpages></addata></record> |
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| subjects | Anesthetics, Intravenous - pharmacology Animals Astrocytes - cytology Cell Death Cell Survival Cells, Cultured Cerebral Cortex - metabolism Cytokines Cytokines - metabolism Dose-Response Relationship, Drug iNOS Interleukin-1beta - metabolism Interleukin-6 - metabolism JNK Lipopolysaccharides - metabolism MAP Kinase Kinase 4 - metabolism Matrix Metalloproteinase 9 - metabolism Neuroglia - cytology Neuroglia - drug effects Neuronal death Neurons - cytology Neurons - metabolism Nitric Oxide - metabolism Nitric Oxide Synthase Type II - metabolism p38 p38 Mitogen-Activated Protein Kinases - metabolism Propofol - pharmacology Rats Rats, Sprague-Dawley Reactive Oxygen Species |
| title | Synergistic activation of lipopolysaccharide-stimulated glial cells by propofol |
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