The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig
Two independent experiments were performed on cyclic (Experiment I) and pregnant (Experiment II) gilts to examine the effect of human Chorionic Gonadotropin (hCG) administration on day 12 of the estrous cycle/pregnancy on ovarian and endometrial secretory function. Animals were divided into hCG Grou...
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| Veröffentlicht in: | Polish journal of veterinary sciences 2013-01, Vol.16 (2), p.323-332 |
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| creator | Bołzan, E Andronowska, A Bodek, G Morawska-Pucińska, E Krawczyński, K Dabrowski, A Ziecik, A J |
| description | Two independent experiments were performed on cyclic (Experiment I) and pregnant (Experiment II) gilts to examine the effect of human Chorionic Gonadotropin (hCG) administration on day 12 of the estrous cycle/pregnancy on ovarian and endometrial secretory function. Animals were divided into hCG Group (injection of 750 IU hCG) and Control Group (injection of saline). In Experiment I, the prolonged lifespan of the corpus luteum (CL), extended progesterone (P4) production (P < 0.05) and delayed luteolysis were found. In hCG Group increased ratio of PGE2:PGFM during 12 hrs period on day 15 (P < 0.05) of the estrous cycle was observed. In both experiments, higher concentrations of E2 in hCG treated gilts (P < 0.05) on days 14-15 of the estrous cycle/pregnancy were found. In Experiment II, hCG injection did not affect P4, PGE2 and PGFM concentrations in blood plasma, but reduced the number of resorbed embryos on day 30 of pregnancy. In the pregnant hCG treated gilts the immunostaining against von Willebrand Factor (vWF) demonstrated an enhanced (P < 0.05) angiogenesis in CLs and endometrium. Furthermore, the flow cytometry revealed an increased (P < 0.05) viability of cells in CLs of hCG Group. An augmented expression of Steroidogenic Acute Regulatory Protein (STAR; P < 0.05) and LH/hCG receptor mRNA (P < 0.05) in CLs of hCG Group were observed, but an elevated concentration of protein was confirmed only for STAR (P < 0.05). Our studies revealed, for the first time, that administration of hCG affects PGE2:PGFM ratio during the estrous cycle as well as the development of conceptuses through enhanced angiogenesis and decreased luteal apoptosis in early pregnant pigs. |
| doi_str_mv | 10.2478/pjvs-2013-0044 |
| format | Article |
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Animals were divided into hCG Group (injection of 750 IU hCG) and Control Group (injection of saline). In Experiment I, the prolonged lifespan of the corpus luteum (CL), extended progesterone (P4) production (P < 0.05) and delayed luteolysis were found. In hCG Group increased ratio of PGE2:PGFM during 12 hrs period on day 15 (P < 0.05) of the estrous cycle was observed. In both experiments, higher concentrations of E2 in hCG treated gilts (P < 0.05) on days 14-15 of the estrous cycle/pregnancy were found. In Experiment II, hCG injection did not affect P4, PGE2 and PGFM concentrations in blood plasma, but reduced the number of resorbed embryos on day 30 of pregnancy. In the pregnant hCG treated gilts the immunostaining against von Willebrand Factor (vWF) demonstrated an enhanced (P < 0.05) angiogenesis in CLs and endometrium. Furthermore, the flow cytometry revealed an increased (P < 0.05) viability of cells in CLs of hCG Group. An augmented expression of Steroidogenic Acute Regulatory Protein (STAR; P < 0.05) and LH/hCG receptor mRNA (P < 0.05) in CLs of hCG Group were observed, but an elevated concentration of protein was confirmed only for STAR (P < 0.05). Our studies revealed, for the first time, that administration of hCG affects PGE2:PGFM ratio during the estrous cycle as well as the development of conceptuses through enhanced angiogenesis and decreased luteal apoptosis in early pregnant pigs.]]></description><identifier>ISSN: 1505-1773</identifier><identifier>EISSN: 2300-2557</identifier><identifier>DOI: 10.2478/pjvs-2013-0044</identifier><identifier>PMID: 23971201</identifier><language>eng</language><publisher>Germany: Polish Academy of Sciences, Committee of Veterinary Sciences</publisher><subject>Animals ; Chorionic Gonadotropin - pharmacology ; Corpus Luteum - drug effects ; Corpus Luteum - physiology ; Estrous Cycle - physiology ; Female ; Pregnancy ; Pregnancy, Animal - drug effects ; Pregnancy, Animal - physiology ; Swine - embryology ; Swine - physiology</subject><ispartof>Polish journal of veterinary sciences, 2013-01, Vol.16 (2), p.323-332</ispartof><rights>Copyright Polish Academy of Sciences, Committee of Veterinary Sciences 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-28a88366ce69e8359429fb6f7789e78bc6126fdadab0b9578a098cc41eb72053</citedby><cites>FETCH-LOGICAL-c363t-28a88366ce69e8359429fb6f7789e78bc6126fdadab0b9578a098cc41eb72053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23971201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bołzan, E</creatorcontrib><creatorcontrib>Andronowska, A</creatorcontrib><creatorcontrib>Bodek, G</creatorcontrib><creatorcontrib>Morawska-Pucińska, E</creatorcontrib><creatorcontrib>Krawczyński, K</creatorcontrib><creatorcontrib>Dabrowski, A</creatorcontrib><creatorcontrib>Ziecik, A J</creatorcontrib><title>The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig</title><title>Polish journal of veterinary sciences</title><addtitle>Pol J Vet Sci</addtitle><description><![CDATA[Two independent experiments were performed on cyclic (Experiment I) and pregnant (Experiment II) gilts to examine the effect of human Chorionic Gonadotropin (hCG) administration on day 12 of the estrous cycle/pregnancy on ovarian and endometrial secretory function. Animals were divided into hCG Group (injection of 750 IU hCG) and Control Group (injection of saline). In Experiment I, the prolonged lifespan of the corpus luteum (CL), extended progesterone (P4) production (P < 0.05) and delayed luteolysis were found. In hCG Group increased ratio of PGE2:PGFM during 12 hrs period on day 15 (P < 0.05) of the estrous cycle was observed. In both experiments, higher concentrations of E2 in hCG treated gilts (P < 0.05) on days 14-15 of the estrous cycle/pregnancy were found. In Experiment II, hCG injection did not affect P4, PGE2 and PGFM concentrations in blood plasma, but reduced the number of resorbed embryos on day 30 of pregnancy. In the pregnant hCG treated gilts the immunostaining against von Willebrand Factor (vWF) demonstrated an enhanced (P < 0.05) angiogenesis in CLs and endometrium. Furthermore, the flow cytometry revealed an increased (P < 0.05) viability of cells in CLs of hCG Group. An augmented expression of Steroidogenic Acute Regulatory Protein (STAR; P < 0.05) and LH/hCG receptor mRNA (P < 0.05) in CLs of hCG Group were observed, but an elevated concentration of protein was confirmed only for STAR (P < 0.05). Our studies revealed, for the first time, that administration of hCG affects PGE2:PGFM ratio during the estrous cycle as well as the development of conceptuses through enhanced angiogenesis and decreased luteal apoptosis in early pregnant pigs.]]></description><subject>Animals</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Corpus Luteum - drug effects</subject><subject>Corpus Luteum - physiology</subject><subject>Estrous Cycle - physiology</subject><subject>Female</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - drug effects</subject><subject>Pregnancy, Animal - physiology</subject><subject>Swine - embryology</subject><subject>Swine - physiology</subject><issn>1505-1773</issn><issn>2300-2557</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1L5TAYhYMoenVm61ICbmZTzUebpMvh4jiC4ObuS5q-uebSJjVphf6L-cmmfsxCCATCc04OPAhdUnLDSqlux8NrKhihvCCkLI_QhnFCClZV8hhtaEWqgkrJz9B5SgdCRM2VOEVnjNeS5tQG_ds9A_bhFXoM1oKZcLD4eXuPdTc479IU9eSCx_n08wS6x3b25v1p0M5P4LU3gLs5Or_HUy6DnAlzwmYxPdyOEfYrsmDtOww69guGoY1LwB3kX8M4gJ-w8-_Z0e1_oBOr-wQ_P-8LtPtzt9v-LR6f7h-2vx8LwwWfCqa0UlwIA6IGxau6ZLVthZVS1SBVawRlwna60y1p60oqTWplTEmhlYxU_AL9-qgdY3iZ8-ZmcMlA32sPeX1DS6aYUEqSjF5_Qw9hjj6PWynBqpJKnqmbD8rEkFIE24zRDTouDSXNqqpZVTWrqmZVlQNXn7VzO0D3H_9yw98ACV-Row</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Bołzan, E</creator><creator>Andronowska, A</creator><creator>Bodek, G</creator><creator>Morawska-Pucińska, E</creator><creator>Krawczyński, K</creator><creator>Dabrowski, A</creator><creator>Ziecik, A J</creator><general>Polish Academy of Sciences, Committee of Veterinary Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20130101</creationdate><title>The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig</title><author>Bołzan, E ; Andronowska, A ; Bodek, G ; Morawska-Pucińska, E ; Krawczyński, K ; Dabrowski, A ; Ziecik, A J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-28a88366ce69e8359429fb6f7789e78bc6126fdadab0b9578a098cc41eb72053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Corpus Luteum - drug effects</topic><topic>Corpus Luteum - physiology</topic><topic>Estrous Cycle - physiology</topic><topic>Female</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - drug effects</topic><topic>Pregnancy, Animal - physiology</topic><topic>Swine - embryology</topic><topic>Swine - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bołzan, E</creatorcontrib><creatorcontrib>Andronowska, A</creatorcontrib><creatorcontrib>Bodek, G</creatorcontrib><creatorcontrib>Morawska-Pucińska, E</creatorcontrib><creatorcontrib>Krawczyński, K</creatorcontrib><creatorcontrib>Dabrowski, A</creatorcontrib><creatorcontrib>Ziecik, A J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Polish journal of veterinary sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bołzan, E</au><au>Andronowska, A</au><au>Bodek, G</au><au>Morawska-Pucińska, E</au><au>Krawczyński, K</au><au>Dabrowski, A</au><au>Ziecik, A J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig</atitle><jtitle>Polish journal of veterinary sciences</jtitle><addtitle>Pol J Vet Sci</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>16</volume><issue>2</issue><spage>323</spage><epage>332</epage><pages>323-332</pages><issn>1505-1773</issn><eissn>2300-2557</eissn><abstract><![CDATA[Two independent experiments were performed on cyclic (Experiment I) and pregnant (Experiment II) gilts to examine the effect of human Chorionic Gonadotropin (hCG) administration on day 12 of the estrous cycle/pregnancy on ovarian and endometrial secretory function. Animals were divided into hCG Group (injection of 750 IU hCG) and Control Group (injection of saline). In Experiment I, the prolonged lifespan of the corpus luteum (CL), extended progesterone (P4) production (P < 0.05) and delayed luteolysis were found. In hCG Group increased ratio of PGE2:PGFM during 12 hrs period on day 15 (P < 0.05) of the estrous cycle was observed. In both experiments, higher concentrations of E2 in hCG treated gilts (P < 0.05) on days 14-15 of the estrous cycle/pregnancy were found. In Experiment II, hCG injection did not affect P4, PGE2 and PGFM concentrations in blood plasma, but reduced the number of resorbed embryos on day 30 of pregnancy. In the pregnant hCG treated gilts the immunostaining against von Willebrand Factor (vWF) demonstrated an enhanced (P < 0.05) angiogenesis in CLs and endometrium. Furthermore, the flow cytometry revealed an increased (P < 0.05) viability of cells in CLs of hCG Group. An augmented expression of Steroidogenic Acute Regulatory Protein (STAR; P < 0.05) and LH/hCG receptor mRNA (P < 0.05) in CLs of hCG Group were observed, but an elevated concentration of protein was confirmed only for STAR (P < 0.05). Our studies revealed, for the first time, that administration of hCG affects PGE2:PGFM ratio during the estrous cycle as well as the development of conceptuses through enhanced angiogenesis and decreased luteal apoptosis in early pregnant pigs.]]></abstract><cop>Germany</cop><pub>Polish Academy of Sciences, Committee of Veterinary Sciences</pub><pmid>23971201</pmid><doi>10.2478/pjvs-2013-0044</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Chorionic Gonadotropin - pharmacology Corpus Luteum - drug effects Corpus Luteum - physiology Estrous Cycle - physiology Female Pregnancy Pregnancy, Animal - drug effects Pregnancy, Animal - physiology Swine - embryology Swine - physiology |
| title | The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig |
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