Light-scattering investigation of the subunit structure and sequential dissociation of Homarus americanus hemocyanin
The hemocyanin of the North American lobster Homarus americanus has a dodecameric subunit structure organized from two basic hexameric units characteristic of arthropod species. Various neutral salts and ureas have been found to dissociate this hemocyanin. The molecular weight profiles studied as a...
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Veröffentlicht in: | Biochemistry (Easton) 1984-01, Vol.23 (8), p.1875-1881 |
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container_title | Biochemistry (Easton) |
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creator | Herskovits, Theodore T Russell, Mark W Carberry, Susan E |
description | The hemocyanin of the North American lobster Homarus americanus has a dodecameric subunit structure organized from two basic hexameric units characteristic of arthropod species. Various neutral salts and ureas have been found to dissociate this hemocyanin. The molecular weight profiles studied as a function of reagent concentration and constant hemocyanin concentration, using light-scattering intensity methods, suggest a two-step reaction of dissociation and assembly of the functional dodecameric protein. |
doi_str_mv | 10.1021/bi00303a045 |
format | Article |
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Various neutral salts and ureas have been found to dissociate this hemocyanin. The molecular weight profiles studied as a function of reagent concentration and constant hemocyanin concentration, using light-scattering intensity methods, suggest a two-step reaction of dissociation and assembly of the functional dodecameric protein.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00303a045</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; dissociation ; Fundamental and applied biological sciences. Psychology ; hemocyanin ; hemocyanins ; Homarus americanus ; light scattering ; Marine ; Metalloproteins ; Other metalloproteins ; Proteins ; structural analysis</subject><ispartof>Biochemistry (Easton), 1984-01, Vol.23 (8), p.1875-1881</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a384t-be0e898f75972403572726acff45366c8a5dbb2bca5f54cb95d94eab8686f0753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00303a045$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00303a045$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8910035$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Herskovits, Theodore T</creatorcontrib><creatorcontrib>Russell, Mark W</creatorcontrib><creatorcontrib>Carberry, Susan E</creatorcontrib><creatorcontrib>Selskostopanska Akademiya, Sofia (Bulgaria)</creatorcontrib><title>Light-scattering investigation of the subunit structure and sequential dissociation of Homarus americanus hemocyanin</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>The hemocyanin of the North American lobster Homarus americanus has a dodecameric subunit structure organized from two basic hexameric units characteristic of arthropod species. Various neutral salts and ureas have been found to dissociate this hemocyanin. The molecular weight profiles studied as a function of reagent concentration and constant hemocyanin concentration, using light-scattering intensity methods, suggest a two-step reaction of dissociation and assembly of the functional dodecameric protein.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>dissociation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hemocyanin</subject><subject>hemocyanins</subject><subject>Homarus americanus</subject><subject>light scattering</subject><subject>Marine</subject><subject>Metalloproteins</subject><subject>Other metalloproteins</subject><subject>Proteins</subject><subject>structural analysis</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNpt0M1rFDEYBvBBFFyrJ-8yB9GDjL4z-ZyjlH4oCyptz-GdbLKbOpvUvBmx_70pWxYPnpKQXx7ePE3zuoePPQz9pykAMGAIXDxpVr0YoOPjKJ42KwCQ3TBKeN68ILqtRw6Kr5qyDttd6chiKS6HuG1D_O2ohC2WkGKbfFt2rqVlWmIoLZW82LJk12LctOR-LS6WgHO7CUTJhuOjy7THvFCL-5pqMdbtzu2TvccY4svmmceZ3KvH9aS5OT-7Pr3s1t8uvpx-XnfINC_d5MDpUXslRjVwYEINapBoveeCSWk1is00DZNF4QW30yg2I3c4aamlByXYSfPukHuXU52UitkHsm6eMbq0kOn5oEFyVuGHA7Q5EWXnzV0O9QP3pgfz0Kz5p9mq3z7GYu1t9hmjDXR8ose-2gfWHVig4v4crzH_NFIxJcz19yujvkp-ztmF-VH9m4P3mAxuc428uepHLQAE04Oq4P0BoCVzm5Yca3n_nfAvf9CfSQ</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Herskovits, Theodore T</creator><creator>Russell, Mark W</creator><creator>Carberry, Susan E</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>19840101</creationdate><title>Light-scattering investigation of the subunit structure and sequential dissociation of Homarus americanus hemocyanin</title><author>Herskovits, Theodore T ; Russell, Mark W ; Carberry, Susan E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a384t-be0e898f75972403572726acff45366c8a5dbb2bca5f54cb95d94eab8686f0753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>dissociation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hemocyanin</topic><topic>hemocyanins</topic><topic>Homarus americanus</topic><topic>light scattering</topic><topic>Marine</topic><topic>Metalloproteins</topic><topic>Other metalloproteins</topic><topic>Proteins</topic><topic>structural analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herskovits, Theodore T</creatorcontrib><creatorcontrib>Russell, Mark W</creatorcontrib><creatorcontrib>Carberry, Susan E</creatorcontrib><creatorcontrib>Selskostopanska Akademiya, Sofia (Bulgaria)</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herskovits, Theodore T</au><au>Russell, Mark W</au><au>Carberry, Susan E</au><aucorp>Selskostopanska Akademiya, Sofia (Bulgaria)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Light-scattering investigation of the subunit structure and sequential dissociation of Homarus americanus hemocyanin</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>23</volume><issue>8</issue><spage>1875</spage><epage>1881</epage><pages>1875-1881</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>The hemocyanin of the North American lobster Homarus americanus has a dodecameric subunit structure organized from two basic hexameric units characteristic of arthropod species. Various neutral salts and ureas have been found to dissociate this hemocyanin. The molecular weight profiles studied as a function of reagent concentration and constant hemocyanin concentration, using light-scattering intensity methods, suggest a two-step reaction of dissociation and assembly of the functional dodecameric protein.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/bi00303a045</doi><tpages>7</tpages></addata></record> |
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source | ACS Publications |
subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences dissociation Fundamental and applied biological sciences. Psychology hemocyanin hemocyanins Homarus americanus light scattering Marine Metalloproteins Other metalloproteins Proteins structural analysis |
title | Light-scattering investigation of the subunit structure and sequential dissociation of Homarus americanus hemocyanin |
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