In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)
In developing countries, around 80% of the population still resorts on traditional medicine for their primary health care. (Walp.) O.F. Cook, one of these medicinal plants, was found to be particularly rich in isoflavonoids which exhibited, individually, significant estrogenic activity . The possibl...
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Veröffentlicht in: | Journal of Complementary & Integrative Medicine 2013-08, Vol.10 (1), p.63-73 |
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creator | Njamen, Dieudonné Djiogue, Sefirin Zingue, Stephane Mvondo, Marie Alfrede N. Nkeh-Chungag, Benedicta |
description | In developing countries, around 80% of the population still resorts on traditional medicine for their primary health care.
(Walp.) O.F. Cook, one of these medicinal plants, was found to be particularly rich in isoflavonoids which exhibited, individually, significant estrogenic activity
. The possible combined effects of these bioactive isoflavones, as they are naturally found in the crude extracts of
, prompted us to assess their
estrogenicity. We first tested the ability of the extracts to transactivate estrogen receptor alpha (ERα)
using U2OS human osteosarcoma cells. We next investigated their effects
in an uterotrophic assay, using ovariectomized rats treated with the extracts at the doses of 50, 100, 200, and 400 mg/kg BW/d orally for 3 days. Finally, we assessed their ability to relieve hot flushes, using data loggers. At the end of treatments, animals were sacrificed, and organs (mammary glands, vagina, and uteri) were collected for histo-morphometric analyses. The methanol extract significantly and dose-dependently transactivated ERα at all tested doses. All extracts induced significant increases of vaginal and uterine epithelial heights. Only the dichloromethane extract could significantly relieve hot flushes as estradiol. These results indicate that
extracts have estrogen-like effects
, suggesting that its active principles act in synergy when they are taken in combination in the crude extract. These findings, therefore, support the traditional use of
to alleviate some menopausal problems; our previous phytochemical investigations contribute to the standardization of this phytomedicine. |
doi_str_mv | 10.1515/jcim-2013-0018 |
format | Article |
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(Walp.) O.F. Cook, one of these medicinal plants, was found to be particularly rich in isoflavonoids which exhibited, individually, significant estrogenic activity
. The possible combined effects of these bioactive isoflavones, as they are naturally found in the crude extracts of
, prompted us to assess their
estrogenicity. We first tested the ability of the extracts to transactivate estrogen receptor alpha (ERα)
using U2OS human osteosarcoma cells. We next investigated their effects
in an uterotrophic assay, using ovariectomized rats treated with the extracts at the doses of 50, 100, 200, and 400 mg/kg BW/d orally for 3 days. Finally, we assessed their ability to relieve hot flushes, using data loggers. At the end of treatments, animals were sacrificed, and organs (mammary glands, vagina, and uteri) were collected for histo-morphometric analyses. The methanol extract significantly and dose-dependently transactivated ERα at all tested doses. All extracts induced significant increases of vaginal and uterine epithelial heights. Only the dichloromethane extract could significantly relieve hot flushes as estradiol. These results indicate that
extracts have estrogen-like effects
, suggesting that its active principles act in synergy when they are taken in combination in the crude extract. These findings, therefore, support the traditional use of
to alleviate some menopausal problems; our previous phytochemical investigations contribute to the standardization of this phytomedicine.</description><identifier>ISSN: 2194-6329</identifier><identifier>EISSN: 1553-3840</identifier><identifier>DOI: 10.1515/jcim-2013-0018</identifier><identifier>PMID: 23969473</identifier><language>eng</language><publisher>Germany: De Gruyter</publisher><subject>Animals ; Biological organs ; Body temperature ; Cell Line ; Cooks ; Dosage ; Dose-Response Relationship, Drug ; Erythrina - chemistry ; Estrogen Receptor alpha - metabolism ; estrogen-like effect ; Female ; Hot Flashes - drug therapy ; Hot Flashes - metabolism ; hot flushes ; Humans ; Induced ; Isoflavones - pharmacology ; Isoflavones - therapeutic use ; ovariectomized rat ; Ovariectomy ; Phytoestrogens - pharmacology ; Phytoestrogens - therapeutic use ; Phytotherapy ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Rats ; Rats, Wistar ; uterotrophic assay</subject><ispartof>Journal of Complementary & Integrative Medicine, 2013-08, Vol.10 (1), p.63-73</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3453-a8230e49280e5f0d831b06dd8d14b5402f5e98619bf57e4e738e88ea4bb2fb543</citedby><cites>FETCH-LOGICAL-c3453-a8230e49280e5f0d831b06dd8d14b5402f5e98619bf57e4e738e88ea4bb2fb543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.1515/jcim-2013-0018/pdf$$EPDF$$P50$$Gwalterdegruyter$$H</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.1515/jcim-2013-0018/html$$EHTML$$P50$$Gwalterdegruyter$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,31005,66759,68543</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23969473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Njamen, Dieudonné</creatorcontrib><creatorcontrib>Djiogue, Sefirin</creatorcontrib><creatorcontrib>Zingue, Stephane</creatorcontrib><creatorcontrib>Mvondo, Marie Alfrede</creatorcontrib><creatorcontrib>N. Nkeh-Chungag, Benedicta</creatorcontrib><title>In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)</title><title>Journal of Complementary & Integrative Medicine</title><addtitle>J Complement Integr Med</addtitle><description>In developing countries, around 80% of the population still resorts on traditional medicine for their primary health care.
(Walp.) O.F. Cook, one of these medicinal plants, was found to be particularly rich in isoflavonoids which exhibited, individually, significant estrogenic activity
. The possible combined effects of these bioactive isoflavones, as they are naturally found in the crude extracts of
, prompted us to assess their
estrogenicity. We first tested the ability of the extracts to transactivate estrogen receptor alpha (ERα)
using U2OS human osteosarcoma cells. We next investigated their effects
in an uterotrophic assay, using ovariectomized rats treated with the extracts at the doses of 50, 100, 200, and 400 mg/kg BW/d orally for 3 days. Finally, we assessed their ability to relieve hot flushes, using data loggers. At the end of treatments, animals were sacrificed, and organs (mammary glands, vagina, and uteri) were collected for histo-morphometric analyses. The methanol extract significantly and dose-dependently transactivated ERα at all tested doses. All extracts induced significant increases of vaginal and uterine epithelial heights. Only the dichloromethane extract could significantly relieve hot flushes as estradiol. These results indicate that
extracts have estrogen-like effects
, suggesting that its active principles act in synergy when they are taken in combination in the crude extract. These findings, therefore, support the traditional use of
to alleviate some menopausal problems; our previous phytochemical investigations contribute to the standardization of this phytomedicine.</description><subject>Animals</subject><subject>Biological organs</subject><subject>Body temperature</subject><subject>Cell Line</subject><subject>Cooks</subject><subject>Dosage</subject><subject>Dose-Response Relationship, Drug</subject><subject>Erythrina - chemistry</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>estrogen-like effect</subject><subject>Female</subject><subject>Hot Flashes - drug therapy</subject><subject>Hot Flashes - metabolism</subject><subject>hot flushes</subject><subject>Humans</subject><subject>Induced</subject><subject>Isoflavones - pharmacology</subject><subject>Isoflavones - therapeutic use</subject><subject>ovariectomized rat</subject><subject>Ovariectomy</subject><subject>Phytoestrogens - pharmacology</subject><subject>Phytoestrogens - therapeutic use</subject><subject>Phytotherapy</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>uterotrophic assay</subject><issn>2194-6329</issn><issn>1553-3840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>7QJ</sourceid><recordid>eNqdkEtLxDAURoMoOj62LiVLXVTzbBNcifgCwY1u3IS0vR0zTJuatOr8e1NG3QniJvkSzv24HIQOKTmlksqzReXajBHKM0Ko2kAzKiXPuBJkE80Y1SLLOdM7aDfGBSFcc1Zsox3Gda5FwWfo-a7Db-7NY9vV2E15CB5DTOccOldhWw0ufa6wbzB8DCG9I26Cb_FVWA0vwXUW9x763s2dTfn42pa2Agsn-2irscsIB1_3Hnq6vnq8vM3uH27uLi_us4qLtKtVjBMQmikCsiG14rQkeV2rmopSCsIaCVrlVJeNLEBAwRUoBVaUJWsSwPfQ8bq3D_51TKub1sUKlkvbgR-joUJoomWR539AWVGIIpcTerpGq-BjDNCYPrjWhpWhxEzqzaTeTOrNpD4NHH11j2UL9Q_-7ToB52vg3S4HCDXMw7hKwSz8GLpk6JdmSmg-Tev_TvNP50OlaQ</recordid><startdate>20130822</startdate><enddate>20130822</enddate><creator>Njamen, Dieudonné</creator><creator>Djiogue, Sefirin</creator><creator>Zingue, Stephane</creator><creator>Mvondo, Marie Alfrede</creator><creator>N. Nkeh-Chungag, Benedicta</creator><general>De Gruyter</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QJ</scope></search><sort><creationdate>20130822</creationdate><title>In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)</title><author>Njamen, Dieudonné ; Djiogue, Sefirin ; Zingue, Stephane ; Mvondo, Marie Alfrede ; N. Nkeh-Chungag, Benedicta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3453-a8230e49280e5f0d831b06dd8d14b5402f5e98619bf57e4e738e88ea4bb2fb543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological organs</topic><topic>Body temperature</topic><topic>Cell Line</topic><topic>Cooks</topic><topic>Dosage</topic><topic>Dose-Response Relationship, Drug</topic><topic>Erythrina - chemistry</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>estrogen-like effect</topic><topic>Female</topic><topic>Hot Flashes - drug therapy</topic><topic>Hot Flashes - metabolism</topic><topic>hot flushes</topic><topic>Humans</topic><topic>Induced</topic><topic>Isoflavones - pharmacology</topic><topic>Isoflavones - therapeutic use</topic><topic>ovariectomized rat</topic><topic>Ovariectomy</topic><topic>Phytoestrogens - pharmacology</topic><topic>Phytoestrogens - therapeutic use</topic><topic>Phytotherapy</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>uterotrophic assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Njamen, Dieudonné</creatorcontrib><creatorcontrib>Djiogue, Sefirin</creatorcontrib><creatorcontrib>Zingue, Stephane</creatorcontrib><creatorcontrib>Mvondo, Marie Alfrede</creatorcontrib><creatorcontrib>N. Nkeh-Chungag, Benedicta</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Applied Social Sciences Index & Abstracts (ASSIA)</collection><jtitle>Journal of Complementary & Integrative Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Njamen, Dieudonné</au><au>Djiogue, Sefirin</au><au>Zingue, Stephane</au><au>Mvondo, Marie Alfrede</au><au>N. Nkeh-Chungag, Benedicta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)</atitle><jtitle>Journal of Complementary & Integrative Medicine</jtitle><addtitle>J Complement Integr Med</addtitle><date>2013-08-22</date><risdate>2013</risdate><volume>10</volume><issue>1</issue><spage>63</spage><epage>73</epage><pages>63-73</pages><issn>2194-6329</issn><eissn>1553-3840</eissn><abstract>In developing countries, around 80% of the population still resorts on traditional medicine for their primary health care.
(Walp.) O.F. Cook, one of these medicinal plants, was found to be particularly rich in isoflavonoids which exhibited, individually, significant estrogenic activity
. The possible combined effects of these bioactive isoflavones, as they are naturally found in the crude extracts of
, prompted us to assess their
estrogenicity. We first tested the ability of the extracts to transactivate estrogen receptor alpha (ERα)
using U2OS human osteosarcoma cells. We next investigated their effects
in an uterotrophic assay, using ovariectomized rats treated with the extracts at the doses of 50, 100, 200, and 400 mg/kg BW/d orally for 3 days. Finally, we assessed their ability to relieve hot flushes, using data loggers. At the end of treatments, animals were sacrificed, and organs (mammary glands, vagina, and uteri) were collected for histo-morphometric analyses. The methanol extract significantly and dose-dependently transactivated ERα at all tested doses. All extracts induced significant increases of vaginal and uterine epithelial heights. Only the dichloromethane extract could significantly relieve hot flushes as estradiol. These results indicate that
extracts have estrogen-like effects
, suggesting that its active principles act in synergy when they are taken in combination in the crude extract. These findings, therefore, support the traditional use of
to alleviate some menopausal problems; our previous phytochemical investigations contribute to the standardization of this phytomedicine.</abstract><cop>Germany</cop><pub>De Gruyter</pub><pmid>23969473</pmid><doi>10.1515/jcim-2013-0018</doi><tpages>11</tpages></addata></record> |
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source | MEDLINE; Applied Social Sciences Index & Abstracts (ASSIA); De Gruyter journals |
subjects | Animals Biological organs Body temperature Cell Line Cooks Dosage Dose-Response Relationship, Drug Erythrina - chemistry Estrogen Receptor alpha - metabolism estrogen-like effect Female Hot Flashes - drug therapy Hot Flashes - metabolism hot flushes Humans Induced Isoflavones - pharmacology Isoflavones - therapeutic use ovariectomized rat Ovariectomy Phytoestrogens - pharmacology Phytoestrogens - therapeutic use Phytotherapy Plant Extracts - chemistry Plant Extracts - pharmacology Plant Extracts - therapeutic use Rats Rats, Wistar uterotrophic assay |
title | In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae) |
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