Purification and Partial Characterization of a Novel β-1,3-Endoglucanase from Streptomyces rutgersensis

A β-1,3-endoglucanase produced by Streptomyces rutgersensis was purified to a homogeneity by the fractional precipitation with ammonium sulfate, ion exchange chromatography on Q-Sepharose and hydrophobic chromatography on Butyl Sepharose. A typical procedure provided 11.74-fold purification with 12....

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Veröffentlicht in:	Protein Journal 2013-06, Vol.32 (5), p.411-417
Hauptverfasser:	Javmen, Artur, Grigiškis, Saulius, Rudenkov, Mark, Mauricas, Mykolas
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Anatomy Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Biochemistry Bioorganic Chemistry Cellulase - chemistry Cellulase - isolation & purification Cellulase - metabolism Chemistry Chemistry and Materials Science Enzyme Stability Histology Hot Temperature Hydrogen-Ion Concentration Isoelectric Point Molecular Weight Morphology Organic Chemistry Saccharomyces cerevisiae Streptomyces Streptomyces - chemistry Streptomyces - enzymology Substrate Specificity
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