Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin
The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 gr...
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| Veröffentlicht in: | Sheng li hsüeh pao 2013-08, Vol.65 (4), p.363-369 |
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| container_title | Sheng li hsüeh pao |
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| creator | Zhang, Tian-Jie Han, Jian-Zhong Liu, Hui-Jun Liao, Xiao-Hong Li, Chen Luo, Zi-Qiang |
| description | The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secreti |
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Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secreti</description><identifier>ISSN: 0371-0874</identifier><identifier>PMID: 23963067</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Cell Line ; Gene Expression ; Interleukin-10 - metabolism ; Lipopolysaccharides ; Macrophages - metabolism ; Mice ; Peptide Fragments - metabolism ; RNA, Messenger ; Uteroglobin - metabolism</subject><ispartof>Sheng li hsüeh pao, 2013-08, Vol.65 (4), p.363-369</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23963067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Tian-Jie</creatorcontrib><creatorcontrib>Han, Jian-Zhong</creatorcontrib><creatorcontrib>Liu, Hui-Jun</creatorcontrib><creatorcontrib>Liao, Xiao-Hong</creatorcontrib><creatorcontrib>Li, Chen</creatorcontrib><creatorcontrib>Luo, Zi-Qiang</creatorcontrib><title>Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin</title><title>Sheng li hsüeh pao</title><addtitle>Sheng Li Xue Bao</addtitle><description>The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secreti</description><subject>Animals</subject><subject>Cell Line</subject><subject>Gene Expression</subject><subject>Interleukin-10 - metabolism</subject><subject>Lipopolysaccharides</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Peptide Fragments - metabolism</subject><subject>RNA, Messenger</subject><subject>Uteroglobin - metabolism</subject><issn>0371-0874</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1rwzAMhnPYWEvXvzB83CXDib_iYyn7KAQGZWPHEMdyMSR2FjvQ3vfD57AOJISkR9KLbrI1JqLIcSXoKtuGYBVmBRGUVuIuW5VEcoK5WGc_R98D8gbNESZ_6r2yLk-urTuhcfIRrEPJWhet6dthSO1iaQw-LsihzguM4DxOkI74BdQoQDdBXLI0edx9lZw-CdRB34dU0XMHGqkLAqd99Gfr7rNb0_YBtte4yT5fnj_2b3n9_nrY7-p8LEoe85YQXhZCMsM6TaXpuDKmE0ZLLJjpcClUxTRnHChAK1UlGCRaa1xRpogkm-zxb2_S_z1DiM1gwyKrdeDn0BS0FBhjJkVCH67orAbQzTjZoZ0uzf_nyC-aYmru</recordid><startdate>20130825</startdate><enddate>20130825</enddate><creator>Zhang, Tian-Jie</creator><creator>Han, Jian-Zhong</creator><creator>Liu, Hui-Jun</creator><creator>Liao, Xiao-Hong</creator><creator>Li, Chen</creator><creator>Luo, Zi-Qiang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20130825</creationdate><title>Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin</title><author>Zhang, Tian-Jie ; Han, Jian-Zhong ; Liu, Hui-Jun ; Liao, Xiao-Hong ; Li, Chen ; Luo, Zi-Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-a33621795f5cd49fc6bffc7fd9075fc027b85d656e4eea9b875e795dd0845b393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Gene Expression</topic><topic>Interleukin-10 - metabolism</topic><topic>Lipopolysaccharides</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Peptide Fragments - metabolism</topic><topic>RNA, Messenger</topic><topic>Uteroglobin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Tian-Jie</creatorcontrib><creatorcontrib>Han, Jian-Zhong</creatorcontrib><creatorcontrib>Liu, Hui-Jun</creatorcontrib><creatorcontrib>Liao, Xiao-Hong</creatorcontrib><creatorcontrib>Li, Chen</creatorcontrib><creatorcontrib>Luo, Zi-Qiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sheng li hsüeh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Tian-Jie</au><au>Han, Jian-Zhong</au><au>Liu, Hui-Jun</au><au>Liao, Xiao-Hong</au><au>Li, Chen</au><au>Luo, Zi-Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin</atitle><jtitle>Sheng li hsüeh pao</jtitle><addtitle>Sheng Li Xue Bao</addtitle><date>2013-08-25</date><risdate>2013</risdate><volume>65</volume><issue>4</issue><spage>363</spage><epage>369</epage><pages>363-369</pages><issn>0371-0874</issn><abstract>The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secreti</abstract><cop>China</cop><pmid>23963067</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Sheng li hsüeh pao, 2013-08, Vol.65 (4), p.363-369 |
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| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Line Gene Expression Interleukin-10 - metabolism Lipopolysaccharides Macrophages - metabolism Mice Peptide Fragments - metabolism RNA, Messenger Uteroglobin - metabolism |
| title | Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin |
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