Delayed Repair of DNA Single-strand Breaks Does Not Increase Cytogenetic Damage
Summary DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (...
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| Veröffentlicht in: | International journal of radiation biology 1985-01, Vol.48 (5), p.711-721 |
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| container_end_page | 721 |
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| container_issue | 5 |
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| container_title | International journal of radiation biology |
| container_volume | 48 |
| creator | Morgan, W.F. Djordjevic, M.C. Jostes, R.F. Pantelias, G.E. |
| description | Summary
DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (ssb). X-radiation (2·5 Gy) induced ssb in both CHO cells and unstimulated lymphocytes, and the breaks were repaired within 30 and 90 min, respectively. This rapid repair was delayed by the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB). The cytogenetic effects of the 3AB-induced delay in DNA repair were examined by analysing sister chromatid exchange (SCE) frequency in CHO cells and fragmentation of prematurely condensed chromosomes (PCC) in unstimulated human lymphocytes after 2·5 Gy of X-rays. Although 3AB delayed the rejoining of DNA ssb, this delay did not result in increased cytogenetic damage manifested as either SCE or fragmentation of PCC. These results indicate that the rapidly rejoining DNA ssb are not important in the production of chromosome damage. |
| doi_str_mv | 10.1080/09553008514551811 |
| format | Article |
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DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (ssb). X-radiation (2·5 Gy) induced ssb in both CHO cells and unstimulated lymphocytes, and the breaks were repaired within 30 and 90 min, respectively. This rapid repair was delayed by the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB). The cytogenetic effects of the 3AB-induced delay in DNA repair were examined by analysing sister chromatid exchange (SCE) frequency in CHO cells and fragmentation of prematurely condensed chromosomes (PCC) in unstimulated human lymphocytes after 2·5 Gy of X-rays. Although 3AB delayed the rejoining of DNA ssb, this delay did not result in increased cytogenetic damage manifested as either SCE or fragmentation of PCC. These results indicate that the rapidly rejoining DNA ssb are not important in the production of chromosome damage.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553008514551811</identifier><identifier>PMID: 3877012</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Chromosome Aberrations ; Cricetinae ; Cricetulus ; DNA Repair ; DNA, Single-Stranded - radiation effects ; Female ; Humans ; Lymphocytes - radiation effects ; Ovary ; Sister Chromatid Exchange</subject><ispartof>International journal of radiation biology, 1985-01, Vol.48 (5), p.711-721</ispartof><rights>1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-ba1ef070fab679653aa506086eb31621ea9871249a56536ca1688057a39069933</citedby><cites>FETCH-LOGICAL-c461t-ba1ef070fab679653aa506086eb31621ea9871249a56536ca1688057a39069933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553008514551811$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553008514551811$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,61198,61233,61379,61414</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3877012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morgan, W.F.</creatorcontrib><creatorcontrib>Djordjevic, M.C.</creatorcontrib><creatorcontrib>Jostes, R.F.</creatorcontrib><creatorcontrib>Pantelias, G.E.</creatorcontrib><title>Delayed Repair of DNA Single-strand Breaks Does Not Increase Cytogenetic Damage</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (ssb). X-radiation (2·5 Gy) induced ssb in both CHO cells and unstimulated lymphocytes, and the breaks were repaired within 30 and 90 min, respectively. This rapid repair was delayed by the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB). The cytogenetic effects of the 3AB-induced delay in DNA repair were examined by analysing sister chromatid exchange (SCE) frequency in CHO cells and fragmentation of prematurely condensed chromosomes (PCC) in unstimulated human lymphocytes after 2·5 Gy of X-rays. Although 3AB delayed the rejoining of DNA ssb, this delay did not result in increased cytogenetic damage manifested as either SCE or fragmentation of PCC. These results indicate that the rapidly rejoining DNA ssb are not important in the production of chromosome damage.</description><subject>Animals</subject><subject>Chromosome Aberrations</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>DNA Repair</subject><subject>DNA, Single-Stranded - radiation effects</subject><subject>Female</subject><subject>Humans</subject><subject>Lymphocytes - radiation effects</subject><subject>Ovary</subject><subject>Sister Chromatid Exchange</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EglL4ARyQfOIW2I1rxxEnaHlJqEg8ztE23ZRAEhc7PfTfY9SKCxKnHe23M9KOECcI5wgWLiDXWgFYjSOt0SLuiAEqkyYqkl0x-OFRQ3ogDkP4gKhA2X2xr2yWAaYD8TThhtY8l8-8pNpLV8nJ9Eq-1N2i4ST0nrq5vPZMn0FOHAc5db186Mq4CSzH694tuOO-LuWEWlrwkdirqAl8vJ1D8XZ78zq-Tx6f7h7GV49JOTLYJzNCriCDimYmy41WRBoMWMMzhSZFptxmmI5y0hGaktBYCzojlYPJc6WG4myTu_Tua8WhL9o6lNw01LFbhQJHqdHx3XiIm8PSuxA8V8XS1y35dYFQ_JRY_Ckxek634atZy_Nfx7a1yC83vO4q51t6Z2r695I8Fx9u5bv4-D_p30c8e7U</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Morgan, W.F.</creator><creator>Djordjevic, M.C.</creator><creator>Jostes, R.F.</creator><creator>Pantelias, G.E.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7TM</scope><scope>7TV</scope><scope>7U2</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19850101</creationdate><title>Delayed Repair of DNA Single-strand Breaks Does Not Increase Cytogenetic Damage</title><author>Morgan, W.F. ; Djordjevic, M.C. ; Jostes, R.F. ; Pantelias, G.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-ba1ef070fab679653aa506086eb31621ea9871249a56536ca1688057a39069933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Chromosome Aberrations</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>DNA Repair</topic><topic>DNA, Single-Stranded - radiation effects</topic><topic>Female</topic><topic>Humans</topic><topic>Lymphocytes - radiation effects</topic><topic>Ovary</topic><topic>Sister Chromatid Exchange</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morgan, W.F.</creatorcontrib><creatorcontrib>Djordjevic, M.C.</creatorcontrib><creatorcontrib>Jostes, R.F.</creatorcontrib><creatorcontrib>Pantelias, G.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Pollution Abstracts</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morgan, W.F.</au><au>Djordjevic, M.C.</au><au>Jostes, R.F.</au><au>Pantelias, G.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delayed Repair of DNA Single-strand Breaks Does Not Increase Cytogenetic Damage</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>48</volume><issue>5</issue><spage>711</spage><epage>721</epage><pages>711-721</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
DNA damage and cytogenetic effects of ionizing radiation were investigated in Chinese hamster ovary (CHO) cells and unstimulated human peripheral blood lymphocytes. DNA damage and repair were analysed by alkaline elution under conditions that predominantly measured DNA single-strand breaks (ssb). X-radiation (2·5 Gy) induced ssb in both CHO cells and unstimulated lymphocytes, and the breaks were repaired within 30 and 90 min, respectively. This rapid repair was delayed by the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB). The cytogenetic effects of the 3AB-induced delay in DNA repair were examined by analysing sister chromatid exchange (SCE) frequency in CHO cells and fragmentation of prematurely condensed chromosomes (PCC) in unstimulated human lymphocytes after 2·5 Gy of X-rays. Although 3AB delayed the rejoining of DNA ssb, this delay did not result in increased cytogenetic damage manifested as either SCE or fragmentation of PCC. These results indicate that the rapidly rejoining DNA ssb are not important in the production of chromosome damage.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>3877012</pmid><doi>10.1080/09553008514551811</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1985-01, Vol.48 (5), p.711-721 |
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| language | eng |
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| source | MEDLINE; Taylor & Francis; Taylor & Francis Medical Library - CRKN |
| subjects | Animals Chromosome Aberrations Cricetinae Cricetulus DNA Repair DNA, Single-Stranded - radiation effects Female Humans Lymphocytes - radiation effects Ovary Sister Chromatid Exchange |
| title | Delayed Repair of DNA Single-strand Breaks Does Not Increase Cytogenetic Damage |
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