Stable-Isotope Probing Reveals That Hydrogen Isotope Fractionation in Proteins and Lipids in a Microbial Community Are Different and Species-Specific

The fractionation of hydrogen stable isotopes during lipid biosynthesis is larger in autotrophic than in heterotrophic microorganisms, possibly due to selective incorporation of hydrogen from water into NAD(P)H, resulting in D-depleted lipids. An analogous fractionation should occur during amino aci...

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Veröffentlicht in:	ACS chemical biology 2013-08, Vol.8 (8), p.1755-1763
Hauptverfasser:	Fischer, Curt R, Bowen, Benjamin P, Pan, Chongle, Northen, Trent R, Banfield, Jillian F
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - chemistry Bacteria - classification Bioreactors Chemical Fractionation Deuterium - chemistry Hydrogen - chemistry Lipids - analysis Lipids - chemistry Molecular Probes Proteins - analysis Proteins - chemistry Species Specificity Spectrometry, Mass, Electrospray Ionization
Online-Zugang:	Volltext
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