Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC
Purpose A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determin...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2013-09, Vol.139 (9), p.1539-1549 |
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| container_title | Journal of cancer research and clinical oncology |
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| creator | Xue, Qun Lv, Liting Wan, Chunhua Chen, Buyou Li, Mei Ni, Tingting Liu, Yifei Liu, Yanhua Cong, Xia Zhou, Yiqun Ni, Runzhou Mao, Guoxin |
| description | Purpose
A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determine whether SGTA could serve as a biomarker for stratification and prediction of prognosis in NSCLC.
Methods
Small glutamine-rich tetratricopeptide repeat-containing protein alpha expression was evaluated by Western blot in 8 paired fresh lung cancer tissues and immunohistochemistry on 83 paraffin-embedded sections. The effect of SGTA was assessed by RNA interference in A549 cells. Serum starvation and refeeding, flow cytometry, CCK-8, and tunnel assays were performed.
Results
Small glutamine-rich tetratricopeptide repeat-containing protein alpha was highly expressed in NSCLC and significantly correlated with NSCLC histological differentiation, clinical stage, and Ki-67. Multivariate analysis indicated that SGTA was an independent prognostic factor for NSCLC patients’ survival. The present investigation demonstrated that suppression of SGTA expression resulted in a significant decline of proliferation in A549 cells. Besides, SGTA could abolish the toxicity of cisplatin in A549 cells.
Conclusions
These findings suggested that SGTA might play an important role in promoting the tumorigenesis of NSCLC, and thus be a promising therapeutic target to prevent NSCLC progression. |
| doi_str_mv | 10.1007/s00432-013-1474-5 |
| format | Article |
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A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determine whether SGTA could serve as a biomarker for stratification and prediction of prognosis in NSCLC.
Methods
Small glutamine-rich tetratricopeptide repeat-containing protein alpha expression was evaluated by Western blot in 8 paired fresh lung cancer tissues and immunohistochemistry on 83 paraffin-embedded sections. The effect of SGTA was assessed by RNA interference in A549 cells. Serum starvation and refeeding, flow cytometry, CCK-8, and tunnel assays were performed.
Results
Small glutamine-rich tetratricopeptide repeat-containing protein alpha was highly expressed in NSCLC and significantly correlated with NSCLC histological differentiation, clinical stage, and Ki-67. Multivariate analysis indicated that SGTA was an independent prognostic factor for NSCLC patients’ survival. The present investigation demonstrated that suppression of SGTA expression resulted in a significant decline of proliferation in A549 cells. Besides, SGTA could abolish the toxicity of cisplatin in A549 cells.
Conclusions
These findings suggested that SGTA might play an important role in promoting the tumorigenesis of NSCLC, and thus be a promising therapeutic target to prevent NSCLC progression.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-013-1474-5</identifier><identifier>PMID: 23857189</identifier><identifier>CODEN: JCROD7</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenocarcinoma - metabolism ; Adenocarcinoma - mortality ; Adult ; Aged ; Antineoplastic agents ; Apoptosis ; Biological and medical sciences ; Biomarkers ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Cancer Research ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - mortality ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - mortality ; Carrier Proteins - metabolism ; Cell Cycle ; Cell Proliferation ; Cohort Studies ; Female ; Follow-Up Studies ; Hematology ; Humans ; Immunoenzyme Techniques ; Internal Medicine ; Lung - metabolism ; Lung cancer ; Lung Neoplasms - metabolism ; Lung Neoplasms - mortality ; Male ; Medical prognosis ; Medical sciences ; Medicine ; Medicine & Public Health ; Middle Aged ; Molecular Chaperones ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Neoplasm Grading ; Neoplasm Staging ; Oncology ; Original Paper ; Pharmacology. Drug treatments ; Pneumology ; Prognosis ; Proteins ; Survival Rate ; Tumor Cells, Cultured ; Tumors ; Tumors of the respiratory system and mediastinum</subject><ispartof>Journal of cancer research and clinical oncology, 2013-09, Vol.139 (9), p.1539-1549</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-d76ce22532871a40737f399d08ca30595c84e102e5ab5a89779677b3fc6c1eaf3</citedby><cites>FETCH-LOGICAL-c402t-d76ce22532871a40737f399d08ca30595c84e102e5ab5a89779677b3fc6c1eaf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00432-013-1474-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00432-013-1474-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27632681$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23857189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Qun</creatorcontrib><creatorcontrib>Lv, Liting</creatorcontrib><creatorcontrib>Wan, Chunhua</creatorcontrib><creatorcontrib>Chen, Buyou</creatorcontrib><creatorcontrib>Li, Mei</creatorcontrib><creatorcontrib>Ni, Tingting</creatorcontrib><creatorcontrib>Liu, Yifei</creatorcontrib><creatorcontrib>Liu, Yanhua</creatorcontrib><creatorcontrib>Cong, Xia</creatorcontrib><creatorcontrib>Zhou, Yiqun</creatorcontrib><creatorcontrib>Ni, Runzhou</creatorcontrib><creatorcontrib>Mao, Guoxin</creatorcontrib><title>Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Purpose
A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determine whether SGTA could serve as a biomarker for stratification and prediction of prognosis in NSCLC.
Methods
Small glutamine-rich tetratricopeptide repeat-containing protein alpha expression was evaluated by Western blot in 8 paired fresh lung cancer tissues and immunohistochemistry on 83 paraffin-embedded sections. The effect of SGTA was assessed by RNA interference in A549 cells. Serum starvation and refeeding, flow cytometry, CCK-8, and tunnel assays were performed.
Results
Small glutamine-rich tetratricopeptide repeat-containing protein alpha was highly expressed in NSCLC and significantly correlated with NSCLC histological differentiation, clinical stage, and Ki-67. Multivariate analysis indicated that SGTA was an independent prognostic factor for NSCLC patients’ survival. The present investigation demonstrated that suppression of SGTA expression resulted in a significant decline of proliferation in A549 cells. Besides, SGTA could abolish the toxicity of cisplatin in A549 cells.
Conclusions
These findings suggested that SGTA might play an important role in promoting the tumorigenesis of NSCLC, and thus be a promising therapeutic target to prevent NSCLC progression.</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - mortality</subject><subject>Adult</subject><subject>Aged</subject><subject>Antineoplastic agents</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Cancer Research</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - mortality</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - mortality</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Cycle</subject><subject>Cell Proliferation</subject><subject>Cohort Studies</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Hematology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Internal Medicine</subject><subject>Lung - metabolism</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - mortality</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Molecular Chaperones</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Oncology</subject><subject>Original Paper</subject><subject>Pharmacology. Drug treatments</subject><subject>Pneumology</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Survival Rate</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kV1rFDEYhYModq3-AG8kIIXtxWg-JpOZy7K0VVhU7Ho9vJt5Z5sym4xJRuxf6a81w25VBCGQryfnHHIIec3ZO86Yfh8ZK6UoGJcFL3VZqCdkwecTLqV6ShaMa14owasT8iLGO5b3Sovn5ETIWmleNwvycPlzDBij9Y6C66gZrLMGBhr8gNT3NO5hGOhumBLsrcMiWHNLE6YAKS_9iGOyHdKAI0Kiy82Xr-eF8S5B1nE7Ogaf0GbtYbwFury53lycU4gUqPM_cKAGs7q5N9nsEc3j081qvXpJnvUwRHx1nE_Jt6vLzepDsf58_XF1sS5MyUQqOl0ZFEJJUWsOJdNS97JpOlYbkEw1ytQlciZQwVZB3WjdVFpvZW8qwxF6eUqWB90c4PuEMbV7G-dc4NBPseWlyH8qylJl9O0_6J2fgsvpZir75xwsU_xAmeBjDNi3Y7B7CPctZ-1cXHsors3FtXNx7az85qg8bffY_X7x2FQGzo4AxNxPH8AZG_9wupKiqnnmxIGL-crtMPwV8b_uvwCtka9q</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Xue, Qun</creator><creator>Lv, Liting</creator><creator>Wan, Chunhua</creator><creator>Chen, Buyou</creator><creator>Li, Mei</creator><creator>Ni, Tingting</creator><creator>Liu, Yifei</creator><creator>Liu, Yanhua</creator><creator>Cong, Xia</creator><creator>Zhou, Yiqun</creator><creator>Ni, Runzhou</creator><creator>Mao, Guoxin</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC</title><author>Xue, Qun ; Lv, Liting ; Wan, Chunhua ; Chen, Buyou ; Li, Mei ; Ni, Tingting ; Liu, Yifei ; Liu, Yanhua ; Cong, Xia ; Zhou, Yiqun ; Ni, Runzhou ; Mao, Guoxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-d76ce22532871a40737f399d08ca30595c84e102e5ab5a89779677b3fc6c1eaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - mortality</topic><topic>Adult</topic><topic>Aged</topic><topic>Antineoplastic agents</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Cancer Research</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - mortality</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - mortality</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Cycle</topic><topic>Cell Proliferation</topic><topic>Cohort Studies</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Hematology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Internal Medicine</topic><topic>Lung - metabolism</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - mortality</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Molecular Chaperones</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Oncology</topic><topic>Original Paper</topic><topic>Pharmacology. Drug treatments</topic><topic>Pneumology</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Survival Rate</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xue, Qun</creatorcontrib><creatorcontrib>Lv, Liting</creatorcontrib><creatorcontrib>Wan, Chunhua</creatorcontrib><creatorcontrib>Chen, Buyou</creatorcontrib><creatorcontrib>Li, Mei</creatorcontrib><creatorcontrib>Ni, Tingting</creatorcontrib><creatorcontrib>Liu, Yifei</creatorcontrib><creatorcontrib>Liu, Yanhua</creatorcontrib><creatorcontrib>Cong, Xia</creatorcontrib><creatorcontrib>Zhou, Yiqun</creatorcontrib><creatorcontrib>Ni, Runzhou</creatorcontrib><creatorcontrib>Mao, Guoxin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Qun</au><au>Lv, Liting</au><au>Wan, Chunhua</au><au>Chen, Buyou</au><au>Li, Mei</au><au>Ni, Tingting</au><au>Liu, Yifei</au><au>Liu, Yanhua</au><au>Cong, Xia</au><au>Zhou, Yiqun</au><au>Ni, Runzhou</au><au>Mao, Guoxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>139</volume><issue>9</issue><spage>1539</spage><epage>1549</epage><pages>1539-1549</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><coden>JCROD7</coden><abstract>Purpose
A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determine whether SGTA could serve as a biomarker for stratification and prediction of prognosis in NSCLC.
Methods
Small glutamine-rich tetratricopeptide repeat-containing protein alpha expression was evaluated by Western blot in 8 paired fresh lung cancer tissues and immunohistochemistry on 83 paraffin-embedded sections. The effect of SGTA was assessed by RNA interference in A549 cells. Serum starvation and refeeding, flow cytometry, CCK-8, and tunnel assays were performed.
Results
Small glutamine-rich tetratricopeptide repeat-containing protein alpha was highly expressed in NSCLC and significantly correlated with NSCLC histological differentiation, clinical stage, and Ki-67. Multivariate analysis indicated that SGTA was an independent prognostic factor for NSCLC patients’ survival. The present investigation demonstrated that suppression of SGTA expression resulted in a significant decline of proliferation in A549 cells. Besides, SGTA could abolish the toxicity of cisplatin in A549 cells.
Conclusions
These findings suggested that SGTA might play an important role in promoting the tumorigenesis of NSCLC, and thus be a promising therapeutic target to prevent NSCLC progression.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23857189</pmid><doi>10.1007/s00432-013-1474-5</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of cancer research and clinical oncology, 2013-09, Vol.139 (9), p.1539-1549 |
| issn | 0171-5216 1432-1335 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adenocarcinoma - metabolism Adenocarcinoma - mortality Adult Aged Antineoplastic agents Apoptosis Biological and medical sciences Biomarkers Biomarkers, Tumor - metabolism Blotting, Western Cancer Research Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - mortality Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - mortality Carrier Proteins - metabolism Cell Cycle Cell Proliferation Cohort Studies Female Follow-Up Studies Hematology Humans Immunoenzyme Techniques Internal Medicine Lung - metabolism Lung cancer Lung Neoplasms - metabolism Lung Neoplasms - mortality Male Medical prognosis Medical sciences Medicine Medicine & Public Health Middle Aged Molecular Chaperones Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Neoplasm Grading Neoplasm Staging Oncology Original Paper Pharmacology. Drug treatments Pneumology Prognosis Proteins Survival Rate Tumor Cells, Cultured Tumors Tumors of the respiratory system and mediastinum |
| title | Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC |
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