Specific binding of ( super(3)H-tyr super(8))physalaemin to rat submaxillary gland substance P receptor

Specific binding of ( super(3)H-tyr super(8))physalaemin to rat submaxillary gland substance P receptor

( super(3)H)Physalaemin (( super(3)H)PHY) binds to a single class of noninteracting sites on rat submaxillary gland membranes suspended in high ionic strength media with a K sub(D) of 2.7 nM, a B sub(max) of 240 fmol/mg of protein, and low nonspecific binding. The findings document that ( super(3)H)...

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Veröffentlicht in:Molecular pharmacology 1985-01, Vol.27 (1), p.38-45
Hauptverfasser: Bahouth, S W, Lazaro, D M, Brundish, DE, Musacchio, J M
Format: Artikel
Sprache:eng
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Zusammenfassung:( super(3)H)Physalaemin (( super(3)H)PHY) binds to a single class of noninteracting sites on rat submaxillary gland membranes suspended in high ionic strength media with a K sub(D) of 2.7 nM, a B sub(max) of 240 fmol/mg of protein, and low nonspecific binding. The findings document that ( super(3)H)PHY binding in high ionic strength accurately reflects the pharmacological potencies of agonists on the SP-P receptor.
ISSN:0026-895X