Surface charge engineering of a Bacillus gibsonii subtilisin protease

In proteins, a posttranslational deamidation process converts asparagine (Asn) and glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu), respectively. This process changes the protein net charge affecting enzyme activity, pH optimum, and stability. Understanding th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied microbiology and biotechnology 2013-08, Vol.97 (15), p.6793-6802
Hauptverfasser:	Jakob, Felix, Martinez, Ronny, Mandawe, John, Hellmuth, Hendrik, Siegert, Petra, Maurer, Karl-Heinz, Schwaneberg, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Asparagine Bacillus Bacillus - enzymology Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Engineering Enzymatic activity Enzyme kinetics Enzymes Glutamate Glutamine Hydrogen-Ion Concentration Influence Life Sciences Microbial Genetics and Genomics Microbiology Mutagenesis, Site-Directed Physiology Proteases Protein Engineering Protein synthesis Proteins Studies Subtilisins - chemistry Subtilisins - genetics Surface Properties
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!