Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae
Helicoverpa armigera (Hübner) is a polyphagous Lepidoptera pest which causes great economic losses in crop production worldwide. In contrast to its agricultural importance, advances in the molecular aspects of this insect are quite limited. In the present study, Illumina’s SOLEXA sequencing was adop...
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| Veröffentlicht in: | Molecular biology reports 2012-12, Vol.39 (12), p.11051-11059 |
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| creator | Li, Jigang Li, Xiumin Chen, Yongli Yang, Zhongxiang Guo, Sandui |
| description | Helicoverpa armigera
(Hübner) is a polyphagous Lepidoptera pest which causes great economic losses in crop production worldwide. In contrast to its agricultural importance, advances in the molecular aspects of this insect are quite limited. In the present study, Illumina’s SOLEXA sequencing was adopted to determine the transcriptome of young
H. armigera
larvae. About 7 gigabases of raw sequence data was generated and assembled into 116,601 contigs with an average length of 389 base pairs after data preprocess. 37,352 of these contigs were annotated by searching against Uniref 100 of UniProt database. The annotated sequences were functionally classified into three groups including biological process (15,632 sequences), cellular component (9,562 sequences) and molecular function (19,258 sequences). KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that 1,409 contigs predicted to encode enzymes with enzyme commission numbers were mapped into 220 KEGG pathways in total. Finally, contigs with simple sequence repeats were derived from this dataset. |
| doi_str_mv | 10.1007/s11033-012-2008-y |
| format | Article |
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(Hübner) is a polyphagous Lepidoptera pest which causes great economic losses in crop production worldwide. In contrast to its agricultural importance, advances in the molecular aspects of this insect are quite limited. In the present study, Illumina’s SOLEXA sequencing was adopted to determine the transcriptome of young
H. armigera
larvae. About 7 gigabases of raw sequence data was generated and assembled into 116,601 contigs with an average length of 389 base pairs after data preprocess. 37,352 of these contigs were annotated by searching against Uniref 100 of UniProt database. The annotated sequences were functionally classified into three groups including biological process (15,632 sequences), cellular component (9,562 sequences) and molecular function (19,258 sequences). KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that 1,409 contigs predicted to encode enzymes with enzyme commission numbers were mapped into 220 KEGG pathways in total. Finally, contigs with simple sequence repeats were derived from this dataset.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-012-2008-y</identifier><identifier>PMID: 23065207</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animals ; Biomedical and Life Sciences ; Contig Mapping ; Databases, Genetic ; Enzymes ; Expressed Sequence Tags ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Genes, Insect - genetics ; Genetic Loci - genetics ; Genomics ; Helicoverpa armigera ; Histology ; Insects ; Larva - genetics ; Lepidoptera ; Lepidoptera - genetics ; Life Sciences ; Metabolic Networks and Pathways - genetics ; Microsatellite Repeats - genetics ; Molecular biology ; Molecular Sequence Annotation ; Morphology ; Sequence Analysis, DNA - methods</subject><ispartof>Molecular biology reports, 2012-12, Vol.39 (12), p.11051-11059</ispartof><rights>Springer Science+Business Media Dordrecht 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-e0c21199c541ac113ff9a6c77b6e0cab1f8e4ba3d5f9feb923f9c502d9379bdf3</citedby><cites>FETCH-LOGICAL-c405t-e0c21199c541ac113ff9a6c77b6e0cab1f8e4ba3d5f9feb923f9c502d9379bdf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-012-2008-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-012-2008-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23065207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jigang</creatorcontrib><creatorcontrib>Li, Xiumin</creatorcontrib><creatorcontrib>Chen, Yongli</creatorcontrib><creatorcontrib>Yang, Zhongxiang</creatorcontrib><creatorcontrib>Guo, Sandui</creatorcontrib><title>Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Helicoverpa armigera
(Hübner) is a polyphagous Lepidoptera pest which causes great economic losses in crop production worldwide. In contrast to its agricultural importance, advances in the molecular aspects of this insect are quite limited. In the present study, Illumina’s SOLEXA sequencing was adopted to determine the transcriptome of young
H. armigera
larvae. About 7 gigabases of raw sequence data was generated and assembled into 116,601 contigs with an average length of 389 base pairs after data preprocess. 37,352 of these contigs were annotated by searching against Uniref 100 of UniProt database. The annotated sequences were functionally classified into three groups including biological process (15,632 sequences), cellular component (9,562 sequences) and molecular function (19,258 sequences). KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that 1,409 contigs predicted to encode enzymes with enzyme commission numbers were mapped into 220 KEGG pathways in total. Finally, contigs with simple sequence repeats were derived from this dataset.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Contig Mapping</subject><subject>Databases, Genetic</subject><subject>Enzymes</subject><subject>Expressed Sequence Tags</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation</subject><subject>Genes, Insect - genetics</subject><subject>Genetic Loci - genetics</subject><subject>Genomics</subject><subject>Helicoverpa armigera</subject><subject>Histology</subject><subject>Insects</subject><subject>Larva - genetics</subject><subject>Lepidoptera</subject><subject>Lepidoptera - genetics</subject><subject>Life Sciences</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Microsatellite Repeats - genetics</subject><subject>Molecular biology</subject><subject>Molecular Sequence Annotation</subject><subject>Morphology</subject><subject>Sequence Analysis, DNA - methods</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1PHDEMhiNUBFvgB_RSjdRLLwN2kplMjhWiUAnEAThHnoyDBs3HNtlF3X9PloWqqlT1lIMfv479CPEJ4RQBzFlCBKVKQFlKgKbc7IkFVkaV2prmg1iAAix1U-Gh-JjSEwBoNNWBOJQK6kqCWYibu3ngX1Qk_rnmyffTY9FS4q5YRZqSj_1yNY9c0ETDJvWpmENxxUPv52eOSyoojv0jRyoGis_Ex2I_0JD45O09Eg_fL-7Pr8rr28sf59-uS6-hWpUMXiJa6yuN5BFVCJZqb0xb5xK1GBrWLamuCjZwa6UKmQXZWWVs2wV1JL7ucpdxzv9OKzf2yfMw0MTzOjnUaFWtQZr_oyjrWjW20Rn98hf6NK9j3vyVqmRtjN1SuKN8nFOKHNwy9iPFjUNwWy1up8VlLW6rxW1yz-e35HU7cve7491DBuQOSLk05Yv-MfqfqS_-NJhi</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Li, Jigang</creator><creator>Li, Xiumin</creator><creator>Chen, Yongli</creator><creator>Yang, Zhongxiang</creator><creator>Guo, Sandui</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20121201</creationdate><title>Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae</title><author>Li, Jigang ; Li, Xiumin ; Chen, Yongli ; Yang, Zhongxiang ; Guo, Sandui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-e0c21199c541ac113ff9a6c77b6e0cab1f8e4ba3d5f9feb923f9c502d9379bdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Contig Mapping</topic><topic>Databases, Genetic</topic><topic>Enzymes</topic><topic>Expressed Sequence Tags</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation</topic><topic>Genes, Insect - genetics</topic><topic>Genetic Loci - genetics</topic><topic>Genomics</topic><topic>Helicoverpa armigera</topic><topic>Histology</topic><topic>Insects</topic><topic>Larva - genetics</topic><topic>Lepidoptera</topic><topic>Lepidoptera - genetics</topic><topic>Life Sciences</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Microsatellite Repeats - genetics</topic><topic>Molecular biology</topic><topic>Molecular Sequence Annotation</topic><topic>Morphology</topic><topic>Sequence Analysis, DNA - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jigang</creatorcontrib><creatorcontrib>Li, Xiumin</creatorcontrib><creatorcontrib>Chen, Yongli</creatorcontrib><creatorcontrib>Yang, Zhongxiang</creatorcontrib><creatorcontrib>Guo, Sandui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jigang</au><au>Li, Xiumin</au><au>Chen, Yongli</au><au>Yang, Zhongxiang</au><au>Guo, Sandui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>39</volume><issue>12</issue><spage>11051</spage><epage>11059</epage><pages>11051-11059</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Helicoverpa armigera
(Hübner) is a polyphagous Lepidoptera pest which causes great economic losses in crop production worldwide. In contrast to its agricultural importance, advances in the molecular aspects of this insect are quite limited. In the present study, Illumina’s SOLEXA sequencing was adopted to determine the transcriptome of young
H. armigera
larvae. About 7 gigabases of raw sequence data was generated and assembled into 116,601 contigs with an average length of 389 base pairs after data preprocess. 37,352 of these contigs were annotated by searching against Uniref 100 of UniProt database. The annotated sequences were functionally classified into three groups including biological process (15,632 sequences), cellular component (9,562 sequences) and molecular function (19,258 sequences). KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that 1,409 contigs predicted to encode enzymes with enzyme commission numbers were mapped into 220 KEGG pathways in total. Finally, contigs with simple sequence repeats were derived from this dataset.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23065207</pmid><doi>10.1007/s11033-012-2008-y</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0301-4851 |
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| subjects | Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Contig Mapping Databases, Genetic Enzymes Expressed Sequence Tags Gene Expression Profiling - methods Gene Expression Regulation Genes, Insect - genetics Genetic Loci - genetics Genomics Helicoverpa armigera Histology Insects Larva - genetics Lepidoptera Lepidoptera - genetics Life Sciences Metabolic Networks and Pathways - genetics Microsatellite Repeats - genetics Molecular biology Molecular Sequence Annotation Morphology Sequence Analysis, DNA - methods |
| title | Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae |
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