Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage
Purpose Due to the attractive properties of poly( l -lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases. Methods MSCs were cu...
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| Veröffentlicht in: | Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA sports traumatology, arthroscopy : official journal of the ESSKA, 2013-08, Vol.21 (8), p.1737-1750 |
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| creator | Izal, Iñigo Aranda, Pablo Sanz-Ramos, Patricia Ripalda, Purificación Mora, Gonzalo Granero-Moltó, Froilán Deplaine, Harmony Gómez-Ribelles, José Luis Ferrer, Gloria Gallego Acosta, Victor Ochoa, Ignacio García-Aznar, Jose Manuel Andreu, Enrique J. Monleón-Pradas, Manuel Doblaré, Manuel Prósper, Felipe |
| description | Purpose
Due to the attractive properties of poly(
l
-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases.
Methods
MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation. Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding.
Results
MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite.
Conclusions
Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold. Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component. |
| doi_str_mv | 10.1007/s00167-012-2148-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1419362117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1411630162</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-6d683dad6abc03be4bdcf0d46b87ca35f00e55a6174e1c688f5125103815af933</originalsourceid><addsrcrecordid>eNqNkc2KFDEURoMozjj6AG4k4GZclOZWUqlqd9L4BwNudF2kkpvuDKmkTFJKv4zPaooeRQTBVQI533e5OYQ8BfYSGOtfZcZA9g2DtmlBDI28Ry5BcN70XPT3ySXbifrCOnlBHuV8y1i9it1DctG2gxSyHy7Jj_3qy5qQRkuP66wCnWJAOquU4vfGYHLf0NAZMwZ9PM3K01xwphq9zzSGLbZEf7r2jVe6OE2VduYFzVpZG73Jr-tzwVBcTapl8U6r4mrOxkTLEWlxOa9IMRxcwDotHLZKrVJxXh3wMXlglc_45O68Il_evf28_9DcfHr_cf_mptGCdaWRRg7cKCPVpBmfUExGW2aEnIZeK95ZxrDrlIReIGg5DLaDtgPGB-iU3XF-Ra7PvUuKX1fMZZxd3pZUAeOaRxCw47IF6P8HBcmrmLaiz_9Cb-OaQl2kUoyLQQjoKgVnSqeYc0I7LslVAacR2Lh5Hs-ex-p53DyPsmae3TWv04zmd-KX2Aq0ZyAv26di-mP0P1t_Al7StIk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1403484415</pqid></control><display><type>article</type><title>Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage</title><source>MEDLINE</source><source>Wiley Journals</source><source>SpringerLink Journals</source><creator>Izal, Iñigo ; Aranda, Pablo ; Sanz-Ramos, Patricia ; Ripalda, Purificación ; Mora, Gonzalo ; Granero-Moltó, Froilán ; Deplaine, Harmony ; Gómez-Ribelles, José Luis ; Ferrer, Gloria Gallego ; Acosta, Victor ; Ochoa, Ignacio ; García-Aznar, Jose Manuel ; Andreu, Enrique J. ; Monleón-Pradas, Manuel ; Doblaré, Manuel ; Prósper, Felipe</creator><creatorcontrib>Izal, Iñigo ; Aranda, Pablo ; Sanz-Ramos, Patricia ; Ripalda, Purificación ; Mora, Gonzalo ; Granero-Moltó, Froilán ; Deplaine, Harmony ; Gómez-Ribelles, José Luis ; Ferrer, Gloria Gallego ; Acosta, Victor ; Ochoa, Ignacio ; García-Aznar, Jose Manuel ; Andreu, Enrique J. ; Monleón-Pradas, Manuel ; Doblaré, Manuel ; Prósper, Felipe</creatorcontrib><description>Purpose
Due to the attractive properties of poly(
l
-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases.
Methods
MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation. Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding.
Results
MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite.
Conclusions
Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold. Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component.</description><identifier>ISSN: 0942-2056</identifier><identifier>EISSN: 1433-7347</identifier><identifier>DOI: 10.1007/s00167-012-2148-6</identifier><identifier>PMID: 22864678</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Adult ; Aggrecans - metabolism ; Apoptosis ; Bone marrow ; Cartilage ; Cartilage Diseases - therapy ; Cell Adhesion ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Collagen Type I - metabolism ; Collagen Type X - metabolism ; Experimental Study ; Extracellular Matrix - metabolism ; Humans ; Lactic Acid ; Medicine ; Medicine & Public Health ; Mesenchymal Stromal Cells - cytology ; Microscopy, Electron, Scanning ; Orthopedics ; Permeability ; Polyesters ; Polymers ; Stem cells ; Surgery ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds</subject><ispartof>Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2013-08, Vol.21 (8), p.1737-1750</ispartof><rights>Springer-Verlag 2012</rights><rights>Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-6d683dad6abc03be4bdcf0d46b87ca35f00e55a6174e1c688f5125103815af933</citedby><cites>FETCH-LOGICAL-c405t-6d683dad6abc03be4bdcf0d46b87ca35f00e55a6174e1c688f5125103815af933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00167-012-2148-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00167-012-2148-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22864678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Izal, Iñigo</creatorcontrib><creatorcontrib>Aranda, Pablo</creatorcontrib><creatorcontrib>Sanz-Ramos, Patricia</creatorcontrib><creatorcontrib>Ripalda, Purificación</creatorcontrib><creatorcontrib>Mora, Gonzalo</creatorcontrib><creatorcontrib>Granero-Moltó, Froilán</creatorcontrib><creatorcontrib>Deplaine, Harmony</creatorcontrib><creatorcontrib>Gómez-Ribelles, José Luis</creatorcontrib><creatorcontrib>Ferrer, Gloria Gallego</creatorcontrib><creatorcontrib>Acosta, Victor</creatorcontrib><creatorcontrib>Ochoa, Ignacio</creatorcontrib><creatorcontrib>García-Aznar, Jose Manuel</creatorcontrib><creatorcontrib>Andreu, Enrique J.</creatorcontrib><creatorcontrib>Monleón-Pradas, Manuel</creatorcontrib><creatorcontrib>Doblaré, Manuel</creatorcontrib><creatorcontrib>Prósper, Felipe</creatorcontrib><title>Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage</title><title>Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA</title><addtitle>Knee Surg Sports Traumatol Arthrosc</addtitle><addtitle>Knee Surg Sports Traumatol Arthrosc</addtitle><description>Purpose
Due to the attractive properties of poly(
l
-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases.
Methods
MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation. Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding.
Results
MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite.
Conclusions
Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold. Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component.</description><subject>Acids</subject><subject>Adult</subject><subject>Aggrecans - metabolism</subject><subject>Apoptosis</subject><subject>Bone marrow</subject><subject>Cartilage</subject><subject>Cartilage Diseases - therapy</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type X - metabolism</subject><subject>Experimental Study</subject><subject>Extracellular Matrix - metabolism</subject><subject>Humans</subject><subject>Lactic Acid</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Orthopedics</subject><subject>Permeability</subject><subject>Polyesters</subject><subject>Polymers</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><issn>0942-2056</issn><issn>1433-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkc2KFDEURoMozjj6AG4k4GZclOZWUqlqd9L4BwNudF2kkpvuDKmkTFJKv4zPaooeRQTBVQI533e5OYQ8BfYSGOtfZcZA9g2DtmlBDI28Ry5BcN70XPT3ySXbifrCOnlBHuV8y1i9it1DctG2gxSyHy7Jj_3qy5qQRkuP66wCnWJAOquU4vfGYHLf0NAZMwZ9PM3K01xwphq9zzSGLbZEf7r2jVe6OE2VduYFzVpZG73Jr-tzwVBcTapl8U6r4mrOxkTLEWlxOa9IMRxcwDotHLZKrVJxXh3wMXlglc_45O68Il_evf28_9DcfHr_cf_mptGCdaWRRg7cKCPVpBmfUExGW2aEnIZeK95ZxrDrlIReIGg5DLaDtgPGB-iU3XF-Ra7PvUuKX1fMZZxd3pZUAeOaRxCw47IF6P8HBcmrmLaiz_9Cb-OaQl2kUoyLQQjoKgVnSqeYc0I7LslVAacR2Lh5Hs-ex-p53DyPsmae3TWv04zmd-KX2Aq0ZyAv26di-mP0P1t_Al7StIk</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Izal, Iñigo</creator><creator>Aranda, Pablo</creator><creator>Sanz-Ramos, Patricia</creator><creator>Ripalda, Purificación</creator><creator>Mora, Gonzalo</creator><creator>Granero-Moltó, Froilán</creator><creator>Deplaine, Harmony</creator><creator>Gómez-Ribelles, José Luis</creator><creator>Ferrer, Gloria Gallego</creator><creator>Acosta, Victor</creator><creator>Ochoa, Ignacio</creator><creator>García-Aznar, Jose Manuel</creator><creator>Andreu, Enrique J.</creator><creator>Monleón-Pradas, Manuel</creator><creator>Doblaré, Manuel</creator><creator>Prósper, Felipe</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20130801</creationdate><title>Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage</title><author>Izal, Iñigo ; Aranda, Pablo ; Sanz-Ramos, Patricia ; Ripalda, Purificación ; Mora, Gonzalo ; Granero-Moltó, Froilán ; Deplaine, Harmony ; Gómez-Ribelles, José Luis ; Ferrer, Gloria Gallego ; Acosta, Victor ; Ochoa, Ignacio ; García-Aznar, Jose Manuel ; Andreu, Enrique J. ; Monleón-Pradas, Manuel ; Doblaré, Manuel ; Prósper, Felipe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-6d683dad6abc03be4bdcf0d46b87ca35f00e55a6174e1c688f5125103815af933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acids</topic><topic>Adult</topic><topic>Aggrecans - metabolism</topic><topic>Apoptosis</topic><topic>Bone marrow</topic><topic>Cartilage</topic><topic>Cartilage Diseases - therapy</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type X - metabolism</topic><topic>Experimental Study</topic><topic>Extracellular Matrix - metabolism</topic><topic>Humans</topic><topic>Lactic Acid</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Orthopedics</topic><topic>Permeability</topic><topic>Polyesters</topic><topic>Polymers</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Izal, Iñigo</creatorcontrib><creatorcontrib>Aranda, Pablo</creatorcontrib><creatorcontrib>Sanz-Ramos, Patricia</creatorcontrib><creatorcontrib>Ripalda, Purificación</creatorcontrib><creatorcontrib>Mora, Gonzalo</creatorcontrib><creatorcontrib>Granero-Moltó, Froilán</creatorcontrib><creatorcontrib>Deplaine, Harmony</creatorcontrib><creatorcontrib>Gómez-Ribelles, José Luis</creatorcontrib><creatorcontrib>Ferrer, Gloria Gallego</creatorcontrib><creatorcontrib>Acosta, Victor</creatorcontrib><creatorcontrib>Ochoa, Ignacio</creatorcontrib><creatorcontrib>García-Aznar, Jose Manuel</creatorcontrib><creatorcontrib>Andreu, Enrique J.</creatorcontrib><creatorcontrib>Monleón-Pradas, Manuel</creatorcontrib><creatorcontrib>Doblaré, Manuel</creatorcontrib><creatorcontrib>Prósper, Felipe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Izal, Iñigo</au><au>Aranda, Pablo</au><au>Sanz-Ramos, Patricia</au><au>Ripalda, Purificación</au><au>Mora, Gonzalo</au><au>Granero-Moltó, Froilán</au><au>Deplaine, Harmony</au><au>Gómez-Ribelles, José Luis</au><au>Ferrer, Gloria Gallego</au><au>Acosta, Victor</au><au>Ochoa, Ignacio</au><au>García-Aznar, Jose Manuel</au><au>Andreu, Enrique J.</au><au>Monleón-Pradas, Manuel</au><au>Doblaré, Manuel</au><au>Prósper, Felipe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage</atitle><jtitle>Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA</jtitle><stitle>Knee Surg Sports Traumatol Arthrosc</stitle><addtitle>Knee Surg Sports Traumatol Arthrosc</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>21</volume><issue>8</issue><spage>1737</spage><epage>1750</epage><pages>1737-1750</pages><issn>0942-2056</issn><eissn>1433-7347</eissn><abstract>Purpose
Due to the attractive properties of poly(
l
-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases.
Methods
MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation. Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding.
Results
MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite.
Conclusions
Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold. Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>22864678</pmid><doi>10.1007/s00167-012-2148-6</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0942-2056 |
| ispartof | Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2013-08, Vol.21 (8), p.1737-1750 |
| issn | 0942-2056 1433-7347 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1419362117 |
| source | MEDLINE; Wiley Journals; SpringerLink Journals |
| subjects | Acids Adult Aggrecans - metabolism Apoptosis Bone marrow Cartilage Cartilage Diseases - therapy Cell Adhesion Cell Culture Techniques - methods Cell Differentiation Cell Proliferation Cells, Cultured Collagen Type I - metabolism Collagen Type X - metabolism Experimental Study Extracellular Matrix - metabolism Humans Lactic Acid Medicine Medicine & Public Health Mesenchymal Stromal Cells - cytology Microscopy, Electron, Scanning Orthopedics Permeability Polyesters Polymers Stem cells Surgery Tissue engineering Tissue Engineering - methods Tissue Scaffolds |
| title | Culture of human bone marrow-derived mesenchymal stem cells on of poly(l-lactic acid) scaffolds: potential application for the tissue engineering of cartilage |
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