Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts
Background Conditions for maintaining hematopoietic progenitor cells (HPCs) before cryopreservation remain controversial. An understanding of the impact of time and temperature during nonfrozen storage can contribute to the maintenance of the quality of products, improving transplantation outcomes....
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| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2013-08, Vol.53 (8), p.1834-1842 |
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| container_title | Transfusion (Philadelphia, Pa.) |
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| creator | Fry, Laura Jayne Giner, Sergio Querol Gomez, Susana G. Green, Melanie Anderson, Sally Horder, Jackie McArdle, Stephanie Rees, Robert Madrigal, Jose Alejandro |
| description | Background
Conditions for maintaining hematopoietic progenitor cells (HPCs) before cryopreservation remain controversial. An understanding of the impact of time and temperature during nonfrozen storage can contribute to the maintenance of the quality of products, improving transplantation outcomes. The objective of this study was to determine the influence on cell potency of thawed products from three sources of HPCs after prolonged storage at different temperatures before cryopreservation.
Study Design and Methods
Viable cell counts by flow cytometry and colony‐forming unit (CFU) recoveries were assessed on cord blood (CB), mobilized peripheral blood stem cell (PBSC), and bone marrow (BM) samples over 72 hours using two different storage conditions, refrigerated (4‐8°C) or room temperature (19‐22°C). To determine the effects of delayed freezing on progenitor recoveries, paired samples were evaluated before and after cryopreservation.
Results
All samples maintained at refrigerated temperatures resulted in higher recoveries than those at room temperature in all variables assessed. Specifically, when assessing for CFU yields after thawing, the impact of time on BM resulted in a significant loss as soon as 24 hours (n = 10, 36.4 ± 28.0%, p = 0.003). This decrease was also observed for PBSCs and CB but at 48 hours of fresh storage (PBSCs n = 11, 32.7 ± 26.2%, p = 0.006; CB n = 10, 39.6 ± 26.4%, p = 0.001).
Conclusion
Our data suggest that HPC products are better maintained at refrigerated temperatures before cryopreservation. Delaying cryopreservation should be minimized to avoid significant losses in cell potency. |
| doi_str_mv | 10.1111/trf.12006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1419341231</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1419341231</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4566-ab310a9419bc41aa64dd6f23d93770bf96ab05eb2c6525c03960302b5554d14d3</originalsourceid><addsrcrecordid>eNp10V1r1TAYB_AgijtOL_wCEhBBL7rlpU3by3FwUzxMlIngTUiTp2eZbVOT9Mx-AT-3qedsgmBukovf8xL-CD2n5ISmcxp9e0IZIeIBWtGClxmr6-IhWhGS04xSzo7QkxBuCCGsJvQxOmKcsYrU1Qr9Ots5a-ywxd65HkfoR_AqTh5wiM6rLWA1GGygUzMYrP3sRg8B_E5F6wY8erezBnADMYLHowsxXqvb9Igw6BnbAV9Dr6IbnYVo9VKwhcGm3lhD1-GtV20MT9GjVnUBnh3uY_Tl_O3V-l22-Xjxfn22yXReCJGphlOi6pzWjc6pUiI3RrSMm5qXJWnaWqiGFNAwLQpWaMJrQThhTVEUuaG54cfo9b5vWuPHBCHK3oZlDzWAm4KkqTXPKeM00Zf_0Bs3-SFtt6gqQVFXSb3ZK-1dCB5aOXrbKz9LSuQSjkzhyD_hJPvi0HFqejD38i6NBF4dgApada1Xg7bhrytF-ki1uNO9u7UdzP-fKK8-n9-NzvYVNkT4eV-h_HcpSl4W8uvlheTluvx0-W0jP_DfRsq3Kg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1418419698</pqid></control><display><type>article</type><title>Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Fry, Laura Jayne ; Giner, Sergio Querol ; Gomez, Susana G. ; Green, Melanie ; Anderson, Sally ; Horder, Jackie ; McArdle, Stephanie ; Rees, Robert ; Madrigal, Jose Alejandro</creator><creatorcontrib>Fry, Laura Jayne ; Giner, Sergio Querol ; Gomez, Susana G. ; Green, Melanie ; Anderson, Sally ; Horder, Jackie ; McArdle, Stephanie ; Rees, Robert ; Madrigal, Jose Alejandro</creatorcontrib><description>Background
Conditions for maintaining hematopoietic progenitor cells (HPCs) before cryopreservation remain controversial. An understanding of the impact of time and temperature during nonfrozen storage can contribute to the maintenance of the quality of products, improving transplantation outcomes. The objective of this study was to determine the influence on cell potency of thawed products from three sources of HPCs after prolonged storage at different temperatures before cryopreservation.
Study Design and Methods
Viable cell counts by flow cytometry and colony‐forming unit (CFU) recoveries were assessed on cord blood (CB), mobilized peripheral blood stem cell (PBSC), and bone marrow (BM) samples over 72 hours using two different storage conditions, refrigerated (4‐8°C) or room temperature (19‐22°C). To determine the effects of delayed freezing on progenitor recoveries, paired samples were evaluated before and after cryopreservation.
Results
All samples maintained at refrigerated temperatures resulted in higher recoveries than those at room temperature in all variables assessed. Specifically, when assessing for CFU yields after thawing, the impact of time on BM resulted in a significant loss as soon as 24 hours (n = 10, 36.4 ± 28.0%, p = 0.003). This decrease was also observed for PBSCs and CB but at 48 hours of fresh storage (PBSCs n = 11, 32.7 ± 26.2%, p = 0.006; CB n = 10, 39.6 ± 26.4%, p = 0.001).
Conclusion
Our data suggest that HPC products are better maintained at refrigerated temperatures before cryopreservation. Delaying cryopreservation should be minimized to avoid significant losses in cell potency.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.12006</identifier><identifier>PMID: 23228098</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Cell Count ; Cell Survival ; Cord Blood Stem Cell Transplantation ; Cryopreservation ; Flow Cytometry ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells ; Humans ; Medical sciences ; Peripheral Blood Stem Cell Transplantation ; Temperature ; Time Factors ; Tissue Preservation - methods ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Transfusion (Philadelphia, Pa.), 2013-08, Vol.53 (8), p.1834-1842</ispartof><rights>2012 American Association of Blood Banks</rights><rights>2014 INIST-CNRS</rights><rights>2012 American Association of Blood Banks.</rights><rights>2013 AABB</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4566-ab310a9419bc41aa64dd6f23d93770bf96ab05eb2c6525c03960302b5554d14d3</citedby><cites>FETCH-LOGICAL-c4566-ab310a9419bc41aa64dd6f23d93770bf96ab05eb2c6525c03960302b5554d14d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.12006$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.12006$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27655588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23228098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fry, Laura Jayne</creatorcontrib><creatorcontrib>Giner, Sergio Querol</creatorcontrib><creatorcontrib>Gomez, Susana G.</creatorcontrib><creatorcontrib>Green, Melanie</creatorcontrib><creatorcontrib>Anderson, Sally</creatorcontrib><creatorcontrib>Horder, Jackie</creatorcontrib><creatorcontrib>McArdle, Stephanie</creatorcontrib><creatorcontrib>Rees, Robert</creatorcontrib><creatorcontrib>Madrigal, Jose Alejandro</creatorcontrib><title>Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>Background
Conditions for maintaining hematopoietic progenitor cells (HPCs) before cryopreservation remain controversial. An understanding of the impact of time and temperature during nonfrozen storage can contribute to the maintenance of the quality of products, improving transplantation outcomes. The objective of this study was to determine the influence on cell potency of thawed products from three sources of HPCs after prolonged storage at different temperatures before cryopreservation.
Study Design and Methods
Viable cell counts by flow cytometry and colony‐forming unit (CFU) recoveries were assessed on cord blood (CB), mobilized peripheral blood stem cell (PBSC), and bone marrow (BM) samples over 72 hours using two different storage conditions, refrigerated (4‐8°C) or room temperature (19‐22°C). To determine the effects of delayed freezing on progenitor recoveries, paired samples were evaluated before and after cryopreservation.
Results
All samples maintained at refrigerated temperatures resulted in higher recoveries than those at room temperature in all variables assessed. Specifically, when assessing for CFU yields after thawing, the impact of time on BM resulted in a significant loss as soon as 24 hours (n = 10, 36.4 ± 28.0%, p = 0.003). This decrease was also observed for PBSCs and CB but at 48 hours of fresh storage (PBSCs n = 11, 32.7 ± 26.2%, p = 0.006; CB n = 10, 39.6 ± 26.4%, p = 0.001).
Conclusion
Our data suggest that HPC products are better maintained at refrigerated temperatures before cryopreservation. Delaying cryopreservation should be minimized to avoid significant losses in cell potency.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Cell Count</subject><subject>Cell Survival</subject><subject>Cord Blood Stem Cell Transplantation</subject><subject>Cryopreservation</subject><subject>Flow Cytometry</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Hematopoietic Stem Cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Peripheral Blood Stem Cell Transplantation</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Tissue Preservation - methods</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10V1r1TAYB_AgijtOL_wCEhBBL7rlpU3by3FwUzxMlIngTUiTp2eZbVOT9Mx-AT-3qedsgmBukovf8xL-CD2n5ISmcxp9e0IZIeIBWtGClxmr6-IhWhGS04xSzo7QkxBuCCGsJvQxOmKcsYrU1Qr9Ots5a-ywxd65HkfoR_AqTh5wiM6rLWA1GGygUzMYrP3sRg8B_E5F6wY8erezBnADMYLHowsxXqvb9Igw6BnbAV9Dr6IbnYVo9VKwhcGm3lhD1-GtV20MT9GjVnUBnh3uY_Tl_O3V-l22-Xjxfn22yXReCJGphlOi6pzWjc6pUiI3RrSMm5qXJWnaWqiGFNAwLQpWaMJrQThhTVEUuaG54cfo9b5vWuPHBCHK3oZlDzWAm4KkqTXPKeM00Zf_0Bs3-SFtt6gqQVFXSb3ZK-1dCB5aOXrbKz9LSuQSjkzhyD_hJPvi0HFqejD38i6NBF4dgApada1Xg7bhrytF-ki1uNO9u7UdzP-fKK8-n9-NzvYVNkT4eV-h_HcpSl4W8uvlheTluvx0-W0jP_DfRsq3Kg</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Fry, Laura Jayne</creator><creator>Giner, Sergio Querol</creator><creator>Gomez, Susana G.</creator><creator>Green, Melanie</creator><creator>Anderson, Sally</creator><creator>Horder, Jackie</creator><creator>McArdle, Stephanie</creator><creator>Rees, Robert</creator><creator>Madrigal, Jose Alejandro</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201308</creationdate><title>Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts</title><author>Fry, Laura Jayne ; Giner, Sergio Querol ; Gomez, Susana G. ; Green, Melanie ; Anderson, Sally ; Horder, Jackie ; McArdle, Stephanie ; Rees, Robert ; Madrigal, Jose Alejandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4566-ab310a9419bc41aa64dd6f23d93770bf96ab05eb2c6525c03960302b5554d14d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Cell Count</topic><topic>Cell Survival</topic><topic>Cord Blood Stem Cell Transplantation</topic><topic>Cryopreservation</topic><topic>Flow Cytometry</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Hematopoietic Stem Cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Peripheral Blood Stem Cell Transplantation</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Tissue Preservation - methods</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fry, Laura Jayne</creatorcontrib><creatorcontrib>Giner, Sergio Querol</creatorcontrib><creatorcontrib>Gomez, Susana G.</creatorcontrib><creatorcontrib>Green, Melanie</creatorcontrib><creatorcontrib>Anderson, Sally</creatorcontrib><creatorcontrib>Horder, Jackie</creatorcontrib><creatorcontrib>McArdle, Stephanie</creatorcontrib><creatorcontrib>Rees, Robert</creatorcontrib><creatorcontrib>Madrigal, Jose Alejandro</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fry, Laura Jayne</au><au>Giner, Sergio Querol</au><au>Gomez, Susana G.</au><au>Green, Melanie</au><au>Anderson, Sally</au><au>Horder, Jackie</au><au>McArdle, Stephanie</au><au>Rees, Robert</au><au>Madrigal, Jose Alejandro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2013-08</date><risdate>2013</risdate><volume>53</volume><issue>8</issue><spage>1834</spage><epage>1842</epage><pages>1834-1842</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>Background
Conditions for maintaining hematopoietic progenitor cells (HPCs) before cryopreservation remain controversial. An understanding of the impact of time and temperature during nonfrozen storage can contribute to the maintenance of the quality of products, improving transplantation outcomes. The objective of this study was to determine the influence on cell potency of thawed products from three sources of HPCs after prolonged storage at different temperatures before cryopreservation.
Study Design and Methods
Viable cell counts by flow cytometry and colony‐forming unit (CFU) recoveries were assessed on cord blood (CB), mobilized peripheral blood stem cell (PBSC), and bone marrow (BM) samples over 72 hours using two different storage conditions, refrigerated (4‐8°C) or room temperature (19‐22°C). To determine the effects of delayed freezing on progenitor recoveries, paired samples were evaluated before and after cryopreservation.
Results
All samples maintained at refrigerated temperatures resulted in higher recoveries than those at room temperature in all variables assessed. Specifically, when assessing for CFU yields after thawing, the impact of time on BM resulted in a significant loss as soon as 24 hours (n = 10, 36.4 ± 28.0%, p = 0.003). This decrease was also observed for PBSCs and CB but at 48 hours of fresh storage (PBSCs n = 11, 32.7 ± 26.2%, p = 0.006; CB n = 10, 39.6 ± 26.4%, p = 0.001).
Conclusion
Our data suggest that HPC products are better maintained at refrigerated temperatures before cryopreservation. Delaying cryopreservation should be minimized to avoid significant losses in cell potency.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23228098</pmid><doi>10.1111/trf.12006</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Transfusion (Philadelphia, Pa.), 2013-08, Vol.53 (8), p.1834-1842 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Cell Count Cell Survival Cord Blood Stem Cell Transplantation Cryopreservation Flow Cytometry Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells Humans Medical sciences Peripheral Blood Stem Cell Transplantation Temperature Time Factors Tissue Preservation - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Avoiding room temperature storage and delayed cryopreservation provide better postthaw potency in hematopoietic progenitor cell grafts |
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