Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication

Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication

A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA befo...

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Veröffentlicht in:Molecular and cellular biology 1985-01, Vol.5 (7), p.1785-1790
Hauptverfasser: Deichaite, I, Laver-Rudich, Z, Dorsett, D, Winocour, E
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container_title Molecular and cellular biology
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creator Deichaite, I
Laver-Rudich, Z
Dorsett, D
Winocour, E
description A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA before transfection led to a theta type of replication which generated supercoiled DNA molecules.
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title Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication
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