A new tool for cloning and gene expression in Streptococcus pneumoniae

•A new replicon for cloning and gene expression was established in Streptococcus pneumoniae.•Stability, small size and a multi-cloning site make it a useful genetic tool.•In the new plasmid vectors expression can be regulated by different copy versions.•S. pneumoniae can accommodate simultaneously p...

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Veröffentlicht in:	Plasmid 2013-09, Vol.70 (2), p.247-253
Hauptverfasser:	Domingues, Susana, Aires, Andreia Cunha, Mohedano, Mari Luz, López, Paloma, Arraiano, Cecília M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blotting, Western Cloning, Molecular - methods DNA Copy Number Variations - genetics DNA Primers - genetics Expression vector Gene Expression Genetic Vectors - genetics Non-integrative vector Plasmids - genetics Pneumococcal cloning Replicating plasmid Replicon - genetics Reverse Transcriptase Polymerase Chain Reaction Streptococcus pneumoniae - genetics
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