Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid
Objective To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) FVB and CF1 mice crossed to create embryos used in experiments. Intervention(s) Mouse embryo assay perform...
Gespeichert in:
| Veröffentlicht in: | Fertility and sterility 2013-08, Vol.100 (2), p.544-549 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 549 |
|---|---|
| container_issue | 2 |
| container_start_page | 544 |
| container_title | Fertility and sterility |
| container_volume | 100 |
| creator | Leonard, Phoebe H., M.D Charlesworth, M. Cristine, Ph.D Benson, Linda, B.S Walker, David L., M.Sc Fredrickson, Jolene R., M.S Morbeck, Dean E., Ph.D |
| description | Objective To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) FVB and CF1 mice crossed to create embryos used in experiments. Intervention(s) Mouse embryo assay performed with 5% or 15% HSA (100 mg/mL albumin) from three samples from three separate manufacturers (A, B, C). Main Outcome Measure(s) Blastocyst rates calculated at 96 hours of culture (experiments repeated in triplicate). Result(s) The HSA preparations were desalted to remove stabilizers added during HSA processing, then mass spectrometry was used to determine the relative variation in stabilizer concentrations; the effect of the stabilizer octanoic acid on embryo development was tested. At 5% HSA, all samples had blastocyst rates ≥70%; at 15% HSA, the blastocyst rates for samples B and C were |
| doi_str_mv | 10.1016/j.fertnstert.2013.03.034 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1417535433</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0015028213004500</els_id><sourcerecordid>1417535433</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-156f1debf62dd86dfad6264a9a9c153388a281438d4bedb84a228f89a93710a23</originalsourceid><addsrcrecordid>eNqNkl1vFCEUhonR2G31LyiX3szK97BemNhGq0kTL2q9JQwclHV2aIExrr9eprtq4pUJ4QR4zsvh5SCEKVlTQtXL7TpArlOpbV4zQvmaLEM8QCsqpeqkkvwhWhFCZUeYZifotJQtIUTRnj1GJ4wrwjjtVyh8tjnaIY6x7nGc8G1OFVq8m-391lzA45Ayht2Q9wm7eaxzhlfHdU0_olu4FHD9CrjUe62fkHFy1U4pOmxd9E_Qo2DHAk-P8QzdvHv76eJ9d_Xx8sPFm6vOScJrR6UK1MMQFPNeKx-sV0wJu7EbRyXnWlumqeDaiwH8oIVlTAfdjnlPiWX8DL046LZ33M1QqtnF4mAc7QRpLoYK2ksuBecN1QfU5VRKhmBuc9zZvDeUmMVlszV_XTaLy4YsQ7TUZ8db5mEH_k_ib1sb8PwABJuM_ZJjMTfXTUG2H2Eb0tNGnB8IaG58j5BNcREmBz5mcNX4FP-njtf_iLgxTtHZ8RvsoWzTnKfmtqGmMEPM9dIPSztQTohotfBfqPuzOg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1417535433</pqid></control><display><type>article</type><title>Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Leonard, Phoebe H., M.D ; Charlesworth, M. Cristine, Ph.D ; Benson, Linda, B.S ; Walker, David L., M.Sc ; Fredrickson, Jolene R., M.S ; Morbeck, Dean E., Ph.D</creator><creatorcontrib>Leonard, Phoebe H., M.D ; Charlesworth, M. Cristine, Ph.D ; Benson, Linda, B.S ; Walker, David L., M.Sc ; Fredrickson, Jolene R., M.S ; Morbeck, Dean E., Ph.D</creatorcontrib><description>Objective To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) FVB and CF1 mice crossed to create embryos used in experiments. Intervention(s) Mouse embryo assay performed with 5% or 15% HSA (100 mg/mL albumin) from three samples from three separate manufacturers (A, B, C). Main Outcome Measure(s) Blastocyst rates calculated at 96 hours of culture (experiments repeated in triplicate). Result(s) The HSA preparations were desalted to remove stabilizers added during HSA processing, then mass spectrometry was used to determine the relative variation in stabilizer concentrations; the effect of the stabilizer octanoic acid on embryo development was tested. At 5% HSA, all samples had blastocyst rates ≥70%; at 15% HSA, the blastocyst rates for samples B and C were <50%. Desalting did not affect sample B but did improve the blastocyst rates of sample C. Mass spectrometry revealed high levels of octanoic acid in sample C compared with sample A. The addition of octanoic acid to sample A produced toxicity similar to sample C. Conclusion(s) The stabilizer octanoic acid varies by lot and inhibits embryo development. Because octanoic acid is known to cause disruptions in mitochondrial bioenergetics, reduce intracellular pH, and induce oxidative damage in peripheral tissues, its use in embryo culture should be monitored and limited.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2013.03.034</identifier><identifier>PMID: 23602317</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Albumin ; Animals ; blastocyst ; Caprylates - toxicity ; Culture Media - chemistry ; Culture Media - standards ; Culture Media - toxicity ; Dose-Response Relationship, Drug ; Drug Stability ; embryo culture ; Embryo Culture Techniques - methods ; Embryo Culture Techniques - standards ; Embryo Culture Techniques - statistics & numerical data ; Embryo, Mammalian - cytology ; Embryo, Mammalian - drug effects ; embryogenesis ; embryotoxicity ; energy metabolism ; Excipients - toxicity ; Female ; human serum albumin ; Humans ; Internal Medicine ; laboratory experimentation ; mass spectrometry ; Mice ; mouse embryo assay ; Obstetrics and Gynecology ; octanoic acid ; Quality Control ; Salts - chemistry ; Serum Albumin - chemistry ; Serum Albumin - pharmacology ; Serum Albumin - toxicity ; stabilizers ; toxicity</subject><ispartof>Fertility and sterility, 2013-08, Vol.100 (2), p.544-549</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2013 American Society for Reproductive Medicine</rights><rights>Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-156f1debf62dd86dfad6264a9a9c153388a281438d4bedb84a228f89a93710a23</citedby><cites>FETCH-LOGICAL-c503t-156f1debf62dd86dfad6264a9a9c153388a281438d4bedb84a228f89a93710a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2013.03.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23602317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leonard, Phoebe H., M.D</creatorcontrib><creatorcontrib>Charlesworth, M. Cristine, Ph.D</creatorcontrib><creatorcontrib>Benson, Linda, B.S</creatorcontrib><creatorcontrib>Walker, David L., M.Sc</creatorcontrib><creatorcontrib>Fredrickson, Jolene R., M.S</creatorcontrib><creatorcontrib>Morbeck, Dean E., Ph.D</creatorcontrib><title>Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) FVB and CF1 mice crossed to create embryos used in experiments. Intervention(s) Mouse embryo assay performed with 5% or 15% HSA (100 mg/mL albumin) from three samples from three separate manufacturers (A, B, C). Main Outcome Measure(s) Blastocyst rates calculated at 96 hours of culture (experiments repeated in triplicate). Result(s) The HSA preparations were desalted to remove stabilizers added during HSA processing, then mass spectrometry was used to determine the relative variation in stabilizer concentrations; the effect of the stabilizer octanoic acid on embryo development was tested. At 5% HSA, all samples had blastocyst rates ≥70%; at 15% HSA, the blastocyst rates for samples B and C were <50%. Desalting did not affect sample B but did improve the blastocyst rates of sample C. Mass spectrometry revealed high levels of octanoic acid in sample C compared with sample A. The addition of octanoic acid to sample A produced toxicity similar to sample C. Conclusion(s) The stabilizer octanoic acid varies by lot and inhibits embryo development. Because octanoic acid is known to cause disruptions in mitochondrial bioenergetics, reduce intracellular pH, and induce oxidative damage in peripheral tissues, its use in embryo culture should be monitored and limited.</description><subject>Albumin</subject><subject>Animals</subject><subject>blastocyst</subject><subject>Caprylates - toxicity</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - standards</subject><subject>Culture Media - toxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Stability</subject><subject>embryo culture</subject><subject>Embryo Culture Techniques - methods</subject><subject>Embryo Culture Techniques - standards</subject><subject>Embryo Culture Techniques - statistics & numerical data</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - drug effects</subject><subject>embryogenesis</subject><subject>embryotoxicity</subject><subject>energy metabolism</subject><subject>Excipients - toxicity</subject><subject>Female</subject><subject>human serum albumin</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>laboratory experimentation</subject><subject>mass spectrometry</subject><subject>Mice</subject><subject>mouse embryo assay</subject><subject>Obstetrics and Gynecology</subject><subject>octanoic acid</subject><subject>Quality Control</subject><subject>Salts - chemistry</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - pharmacology</subject><subject>Serum Albumin - toxicity</subject><subject>stabilizers</subject><subject>toxicity</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl1vFCEUhonR2G31LyiX3szK97BemNhGq0kTL2q9JQwclHV2aIExrr9eprtq4pUJ4QR4zsvh5SCEKVlTQtXL7TpArlOpbV4zQvmaLEM8QCsqpeqkkvwhWhFCZUeYZifotJQtIUTRnj1GJ4wrwjjtVyh8tjnaIY6x7nGc8G1OFVq8m-391lzA45Ayht2Q9wm7eaxzhlfHdU0_olu4FHD9CrjUe62fkHFy1U4pOmxd9E_Qo2DHAk-P8QzdvHv76eJ9d_Xx8sPFm6vOScJrR6UK1MMQFPNeKx-sV0wJu7EbRyXnWlumqeDaiwH8oIVlTAfdjnlPiWX8DL046LZ33M1QqtnF4mAc7QRpLoYK2ksuBecN1QfU5VRKhmBuc9zZvDeUmMVlszV_XTaLy4YsQ7TUZ8db5mEH_k_ib1sb8PwABJuM_ZJjMTfXTUG2H2Eb0tNGnB8IaG58j5BNcREmBz5mcNX4FP-njtf_iLgxTtHZ8RvsoWzTnKfmtqGmMEPM9dIPSztQTohotfBfqPuzOg</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Leonard, Phoebe H., M.D</creator><creator>Charlesworth, M. Cristine, Ph.D</creator><creator>Benson, Linda, B.S</creator><creator>Walker, David L., M.Sc</creator><creator>Fredrickson, Jolene R., M.S</creator><creator>Morbeck, Dean E., Ph.D</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130801</creationdate><title>Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid</title><author>Leonard, Phoebe H., M.D ; Charlesworth, M. Cristine, Ph.D ; Benson, Linda, B.S ; Walker, David L., M.Sc ; Fredrickson, Jolene R., M.S ; Morbeck, Dean E., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-156f1debf62dd86dfad6264a9a9c153388a281438d4bedb84a228f89a93710a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Albumin</topic><topic>Animals</topic><topic>blastocyst</topic><topic>Caprylates - toxicity</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - standards</topic><topic>Culture Media - toxicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Stability</topic><topic>embryo culture</topic><topic>Embryo Culture Techniques - methods</topic><topic>Embryo Culture Techniques - standards</topic><topic>Embryo Culture Techniques - statistics & numerical data</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - drug effects</topic><topic>embryogenesis</topic><topic>embryotoxicity</topic><topic>energy metabolism</topic><topic>Excipients - toxicity</topic><topic>Female</topic><topic>human serum albumin</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>laboratory experimentation</topic><topic>mass spectrometry</topic><topic>Mice</topic><topic>mouse embryo assay</topic><topic>Obstetrics and Gynecology</topic><topic>octanoic acid</topic><topic>Quality Control</topic><topic>Salts - chemistry</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin - pharmacology</topic><topic>Serum Albumin - toxicity</topic><topic>stabilizers</topic><topic>toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leonard, Phoebe H., M.D</creatorcontrib><creatorcontrib>Charlesworth, M. Cristine, Ph.D</creatorcontrib><creatorcontrib>Benson, Linda, B.S</creatorcontrib><creatorcontrib>Walker, David L., M.Sc</creatorcontrib><creatorcontrib>Fredrickson, Jolene R., M.S</creatorcontrib><creatorcontrib>Morbeck, Dean E., Ph.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leonard, Phoebe H., M.D</au><au>Charlesworth, M. Cristine, Ph.D</au><au>Benson, Linda, B.S</au><au>Walker, David L., M.Sc</au><au>Fredrickson, Jolene R., M.S</au><au>Morbeck, Dean E., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>100</volume><issue>2</issue><spage>544</spage><epage>549</epage><pages>544-549</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><abstract>Objective To screen human serum albumin (HSA) preparations for toxicity and investigate causes of variation. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) FVB and CF1 mice crossed to create embryos used in experiments. Intervention(s) Mouse embryo assay performed with 5% or 15% HSA (100 mg/mL albumin) from three samples from three separate manufacturers (A, B, C). Main Outcome Measure(s) Blastocyst rates calculated at 96 hours of culture (experiments repeated in triplicate). Result(s) The HSA preparations were desalted to remove stabilizers added during HSA processing, then mass spectrometry was used to determine the relative variation in stabilizer concentrations; the effect of the stabilizer octanoic acid on embryo development was tested. At 5% HSA, all samples had blastocyst rates ≥70%; at 15% HSA, the blastocyst rates for samples B and C were <50%. Desalting did not affect sample B but did improve the blastocyst rates of sample C. Mass spectrometry revealed high levels of octanoic acid in sample C compared with sample A. The addition of octanoic acid to sample A produced toxicity similar to sample C. Conclusion(s) The stabilizer octanoic acid varies by lot and inhibits embryo development. Because octanoic acid is known to cause disruptions in mitochondrial bioenergetics, reduce intracellular pH, and induce oxidative damage in peripheral tissues, its use in embryo culture should be monitored and limited.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23602317</pmid><doi>10.1016/j.fertnstert.2013.03.034</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-0282 |
| ispartof | Fertility and sterility, 2013-08, Vol.100 (2), p.544-549 |
| issn | 0015-0282 1556-5653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1417535433 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Albumin Animals blastocyst Caprylates - toxicity Culture Media - chemistry Culture Media - standards Culture Media - toxicity Dose-Response Relationship, Drug Drug Stability embryo culture Embryo Culture Techniques - methods Embryo Culture Techniques - standards Embryo Culture Techniques - statistics & numerical data Embryo, Mammalian - cytology Embryo, Mammalian - drug effects embryogenesis embryotoxicity energy metabolism Excipients - toxicity Female human serum albumin Humans Internal Medicine laboratory experimentation mass spectrometry Mice mouse embryo assay Obstetrics and Gynecology octanoic acid Quality Control Salts - chemistry Serum Albumin - chemistry Serum Albumin - pharmacology Serum Albumin - toxicity stabilizers toxicity |
| title | Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T18%3A09%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Variability%20in%20protein%20quality%20used%20for%20embryo%20culture:%20embryotoxicity%20of%20the%20stabilizer%20octanoic%20acid&rft.jtitle=Fertility%20and%20sterility&rft.au=Leonard,%20Phoebe%20H.,%20M.D&rft.date=2013-08-01&rft.volume=100&rft.issue=2&rft.spage=544&rft.epage=549&rft.pages=544-549&rft.issn=0015-0282&rft.eissn=1556-5653&rft_id=info:doi/10.1016/j.fertnstert.2013.03.034&rft_dat=%3Cproquest_cross%3E1417535433%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1417535433&rft_id=info:pmid/23602317&rft_els_id=1_s2_0_S0015028213004500&rfr_iscdi=true |