Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1

•Inclusion of ATP and Mg2+ during purificationprevents ScDmc1 aggregation.•ScDmc1 so purified is active as recombinase and responsive to Rad54 and Rdh54.•3D reconstruction of ScDmc1 on ssDNA illustrateslikeness with other recombinases. The role of Dmc1 as a meiosis-specific general recombinase was f...

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Veröffentlicht in:	DNA repair 2013-09, Vol.12 (9), p.707-712
Hauptverfasser:	Busygina, Valeria, Gaines, William A., Xu, Yuanyuan, Kwon, Youngho, Williams, Gareth J., Lin, Sheng-Wei, Chang, Hao-Yen, Chi, Peter, Wang, Hong-Wei, Sung, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - chemistry Calcium - chemistry Cell Cycle Proteins - chemistry Cell Cycle Proteins - isolation & purification Cell Cycle Proteins - physiology Chromatography, Gel Dmc1 recombinase DNA Helicases - chemistry DNA Repair Enzymes - chemistry DNA Topoisomerases - chemistry DNA, Fungal - chemistry DNA, Fungal - ultrastructure DNA, Single-Stranded - chemistry DNA-Binding Proteins - chemistry DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - physiology Homologous Recombination Humans Hydrolysis Meiosis Protein Binding Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - physiology
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