Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5
Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspe...
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| Veröffentlicht in: | Microbial pathogenesis 2013-10, Vol.63, p.30-36 |
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| creator | Xie, Jiexiong Zhu, Wanjun Chen, Ye Wei, Chunya Zhou, Pei Zhang, Minze Huang, Zhen Sun, Long Su, Shuo Zhang, Guihong |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%–83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern. |
| doi_str_mv | 10.1016/j.micpath.2013.05.013 |
| format | Article |
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In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%–83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2013.05.013</identifier><identifier>PMID: 23770054</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>amino acid composition ; amino acids ; Animals ; China - epidemiology ; Cluster Analysis ; farms ; genes ; Genetic diversity ; genetic techniques and protocols ; genetic variation ; Genotype ; GP5 ; Molecular Epidemiology ; Molecular Sequence Data ; mutation ; open reading frames ; Porcine Reproductive and Respiratory Syndrome - epidemiology ; Porcine Reproductive and Respiratory Syndrome - virology ; Porcine reproductive and respiratory syndrome virus ; Porcine reproductive and respiratory syndrome virus (PRRSV) ; Porcine respiratory and reproductive syndrome virus - classification ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - isolation & purification ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; South China ; Swine ; Viral Envelope Proteins - genetics ; viruses</subject><ispartof>Microbial pathogenesis, 2013-10, Vol.63, p.30-36</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-d70bba3045fb7d5779adb5d2df3d4713c6dee1965a120a182ff590c897f6ca73</citedby><cites>FETCH-LOGICAL-c389t-d70bba3045fb7d5779adb5d2df3d4713c6dee1965a120a182ff590c897f6ca73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0882401013000909$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23770054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Jiexiong</creatorcontrib><creatorcontrib>Zhu, Wanjun</creatorcontrib><creatorcontrib>Chen, Ye</creatorcontrib><creatorcontrib>Wei, Chunya</creatorcontrib><creatorcontrib>Zhou, Pei</creatorcontrib><creatorcontrib>Zhang, Minze</creatorcontrib><creatorcontrib>Huang, Zhen</creatorcontrib><creatorcontrib>Sun, Long</creatorcontrib><creatorcontrib>Su, Shuo</creatorcontrib><creatorcontrib>Zhang, Guihong</creatorcontrib><title>Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%–83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern.</description><subject>amino acid composition</subject><subject>amino acids</subject><subject>Animals</subject><subject>China - epidemiology</subject><subject>Cluster Analysis</subject><subject>farms</subject><subject>genes</subject><subject>Genetic diversity</subject><subject>genetic techniques and protocols</subject><subject>genetic variation</subject><subject>Genotype</subject><subject>GP5</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>mutation</subject><subject>open reading frames</subject><subject>Porcine Reproductive and Respiratory Syndrome - epidemiology</subject><subject>Porcine Reproductive and Respiratory Syndrome - virology</subject><subject>Porcine reproductive and respiratory syndrome virus</subject><subject>Porcine reproductive and respiratory syndrome virus (PRRSV)</subject><subject>Porcine respiratory and reproductive syndrome virus - classification</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - isolation & purification</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>South China</subject><subject>Swine</subject><subject>Viral Envelope Proteins - genetics</subject><subject>viruses</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMGO0zAQhi0EYsvCIwA-ckkYO3EcnxCqWEBatKhduFqOPW5dJXGxE6S-PalauHL6L9_8M_MR8ppByYA17w_lEOzRTPuSA6tKEOUST8iKgWoKxqF9SlbQtryogcENeZHzAQBUXann5IZXUgKIekX8t9ijnXuTKB6DwyHEPu5ONHr6fbPZ_qRhpNs4T3u63ofRUJ_iQDmApFNckjHamYyOxpFOe6Q7HHEKlprR9Kcc8rnnYXMnXpJn3vQZX13zljzefXpcfynuHz5_XX-8L2zVqqlwErrOVFAL30knpFTGdcJx5ytXS1bZxiEy1QizfGhYy70XCmyrpG-skdUteXepPab4a8Y86SFki31vRoxz1qxmTaMqJZsFFRfUpphzQq-PKQwmnTQDfTasD_pqWJ8NaxB6iWXuzXXF3A3o_k39VboAby-AN1GbXQpZ_9guDQ0A44K35ys_XAhcTPwOmHS2AUeLLiS0k3Yx_OeIP_flli4</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Xie, Jiexiong</creator><creator>Zhu, Wanjun</creator><creator>Chen, Ye</creator><creator>Wei, Chunya</creator><creator>Zhou, Pei</creator><creator>Zhang, Minze</creator><creator>Huang, Zhen</creator><creator>Sun, Long</creator><creator>Su, Shuo</creator><creator>Zhang, Guihong</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131001</creationdate><title>Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5</title><author>Xie, Jiexiong ; Zhu, Wanjun ; Chen, Ye ; Wei, Chunya ; Zhou, Pei ; Zhang, Minze ; Huang, Zhen ; Sun, Long ; Su, Shuo ; Zhang, Guihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-d70bba3045fb7d5779adb5d2df3d4713c6dee1965a120a182ff590c897f6ca73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>amino acid composition</topic><topic>amino acids</topic><topic>Animals</topic><topic>China - epidemiology</topic><topic>Cluster Analysis</topic><topic>farms</topic><topic>genes</topic><topic>Genetic diversity</topic><topic>genetic techniques and protocols</topic><topic>genetic variation</topic><topic>Genotype</topic><topic>GP5</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>mutation</topic><topic>open reading frames</topic><topic>Porcine Reproductive and Respiratory Syndrome - epidemiology</topic><topic>Porcine Reproductive and Respiratory Syndrome - virology</topic><topic>Porcine reproductive and respiratory syndrome virus</topic><topic>Porcine reproductive and respiratory syndrome virus (PRRSV)</topic><topic>Porcine respiratory and reproductive syndrome virus - classification</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - isolation & purification</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>South China</topic><topic>Swine</topic><topic>Viral Envelope Proteins - genetics</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Jiexiong</creatorcontrib><creatorcontrib>Zhu, Wanjun</creatorcontrib><creatorcontrib>Chen, Ye</creatorcontrib><creatorcontrib>Wei, Chunya</creatorcontrib><creatorcontrib>Zhou, Pei</creatorcontrib><creatorcontrib>Zhang, Minze</creatorcontrib><creatorcontrib>Huang, Zhen</creatorcontrib><creatorcontrib>Sun, Long</creatorcontrib><creatorcontrib>Su, Shuo</creatorcontrib><creatorcontrib>Zhang, Guihong</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Jiexiong</au><au>Zhu, Wanjun</au><au>Chen, Ye</au><au>Wei, Chunya</au><au>Zhou, Pei</au><au>Zhang, Minze</au><au>Huang, Zhen</au><au>Sun, Long</au><au>Su, Shuo</au><au>Zhang, Guihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>63</volume><spage>30</spage><epage>36</epage><pages>30-36</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Porcine reproductive and respiratory syndrome virus (PRRSV) has proven to be highly genetically variable; however, comprehensive information regarding the virus's genetic diversity in South China is limited. In this study, a total of 3199 clinical samples were collected from 267 pig farms suspected of PRRSV infection between 2007 and 2011. The ORF5 genes of 51 PRRSV-positive samples were sequenced and analyzed. The 51 study strains were divided into three primary subgenotypes. Fourty-five of the strains belonged to subgenotype I and were closely related to the highly pathogenic PRRSV (HP-PRRSV) strains. The subgenotype I strains were generally clustered into genetically similar groups by year. Only one of the strains belonged to subgenotype II, clustering with the classical North American type, VR2332. Five of the strains were grouped into subgenotype III, which occupied a separate branch and was closely related to the recently isolated novel field strains, QYYZ and GM2. The 5 subgenotype III strains shared an amino acid identity with the remaining 46 study strains ranging from 79.6%–83.6%. Amino acid analysis showed extensive mutations in subgenotype III; the diverse genetic mutations of these novel strains are of great concern.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23770054</pmid><doi>10.1016/j.micpath.2013.05.013</doi><tpages>7</tpages></addata></record> |
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| subjects | amino acid composition amino acids Animals China - epidemiology Cluster Analysis farms genes Genetic diversity genetic techniques and protocols genetic variation Genotype GP5 Molecular Epidemiology Molecular Sequence Data mutation open reading frames Porcine Reproductive and Respiratory Syndrome - epidemiology Porcine Reproductive and Respiratory Syndrome - virology Porcine reproductive and respiratory syndrome virus Porcine reproductive and respiratory syndrome virus (PRRSV) Porcine respiratory and reproductive syndrome virus - classification Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - isolation & purification RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology, Amino Acid South China Swine Viral Envelope Proteins - genetics viruses |
| title | Molecular epidemiology of PRRSV in South China from 2007 to 2011 based on the genetic analysis of ORF5 |
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